Recombinant Mouse Folliculin-interacting protein 1 (Fnip1), partial

中文名稱：

小鼠Fnip1重組蛋白
貨號(hào)：

CSB-YP734663MO
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

小鼠Fnip1重組蛋白
貨號(hào)：

CSB-EP734663MO
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

小鼠Fnip1重組蛋白
貨號(hào)：

CSB-EP734663MO-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Fnip1重組蛋白
貨號(hào)：

CSB-BP734663MO
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

小鼠Fnip1重組蛋白
貨號(hào)：

CSB-MP734663MO
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Fnip1
Uniprot No.：

Q68FD7
別名：

Fnip1; Kiaa1961Folliculin-interacting protein 1
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Binding partner of the GTPase-activating protein FLCN: involved in the cellular response to amino acid availability by regulating the mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3. In low-amino acid conditions, component of the lysosomal folliculin complex (LFC) on the membrane of lysosomes, which inhibits the GTPase-activating activity of FLCN, thereby inactivating mTORC1 and promoting nuclear translocation of TFEB and TFE3. Upon amino acid restimulation, disassembly of the LFC complex liberates the GTPase-activating activity of FLCN, leading to activation of mTORC1 and subsequent cytoplasmic retention of TFEB and TFE3. Required to promote FLCN recruitment to lysosomes and interaction with Rag GTPases. Together with FLCN, regulates autophagy: following phosphorylation by ULK1, interacts with GABARAP and promotes autophagy. In addition to its role in mTORC1 signaling, also acts as a co-chaperone of HSP90AA1/Hsp90: following gradual phosphorylation by CK2, inhibits the ATPase activity of HSP90AA1/Hsp90, leading to activate both kinase and non-kinase client proteins of HSP90AA1/Hsp90. Acts as a scaffold to load client protein FLCN onto HSP90AA1/Hsp90. Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins. Also acts as a core component of the reductive stress response by inhibiting activation of mitochondria in normal conditions: in response to reductive stress, the conserved Cys degron is reduced, leading to recognition and polyubiquitylation by the CRL2(FEM1B) complex, followed by proteasomal degradation and subsequent activation of mitochondria to recalibrate reactive oxygen species (ROS). Required for B-cell development.
基因功能參考文獻(xiàn)：
1. Loss of Fnip1 alters kidney developmental transcriptional program and synergizes with TSC1 loss to promote mTORC1 activation and renal cyst formation PMID: 29897930
2. These findings suggest that Dihydromyricetin treatment prevents palmitate-induced slow-twitch fibers decrease partially via FLCN-FNIP1-AMPK pathway thereby improving insulin resistance in obesity. PMID: 28363698
3. FNIP1-deficient mice developed cardiomyopathy characterized by left ventricular hypertrophy and glycogen accumulation. PMID: 27303042
4. Fnip1 and Fnip2 play critical roles in kidney tumor suppression in cooperation with Flcn PMID: 25775561
5. loss of Fnip1 increased the representation of type I fibers characterized by increased myoglobin, slow twitch markers [myosin heavy chain 7, succinate dehydrogenase, troponin I 1, troponin C1, troponin T1], capillary density, and mitochondria number PMID: 25548157
6. These results indicate that Fnip1 is vital for invariant natural killer cell development by maintaining metabolic homeostasis in response to metabolic stress. PMID: 24785297
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亞細(xì)胞定位：

Lysosome membrane. Cytoplasm, cytosol.
蛋白家族：

FNIP family
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:216742

STRING: 10090.ENSMUSP00000049026

UniGene: Mm.34087