Recombinant Mouse E3 ubiquitin-protein ligase NEDD4 (Nedd4), partial

中文名稱：

小鼠Nedd4重組蛋白
貨號：

CSB-YP015681MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Nedd4重組蛋白
貨號：

CSB-EP015681MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Nedd4重組蛋白
貨號：

CSB-EP015681MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Nedd4重組蛋白
貨號：

CSB-BP015681MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Nedd4重組蛋白
貨號：

CSB-MP015681MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Nedd4
Uniprot No.：

P46935
別名：

Nedd4; Kiaa0093; Nedd-4; Nedd4-1; Nedd4a; E3 ubiquitin-protein ligase NEDD4; EC 2.3.2.26; HECT-type E3 ubiquitin transferase NEDD4; Neural precursor cell expressed developmentally down-regulated protein 4; NEDD-4
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Involved in ubiquitination of ERBB4 intracellular domain E4ICD1. Predominantly involved in ubiquitination of membrane bound forms of ERBB4 rather than processed precursors and intermediate membrane-anchored 80 kDa fragments (m80HER4), with a lesser role in ubiquitination of ERBB4 intracellular domain E4ICD1. Promotes ubiquitination of RAPGEF2. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1.
基因功能參考文獻：
1. NEDD4 targets an RNA-binding protein, NANOS2, in spermatogonia to destabilize it, leading to cell differentiation. A NEDD4-mediated mechanism regulates mRNP dynamics, and facilitates spermatogonial progenitor cell homeostasis and viability under normal and stress conditions. PMID: 28585553
2. these findings support NEDD4 as a novel regulator of adipogenesis by modulating the stability of PPARgamma. PMID: 27917940
3. expression or dysfunction of Nedd4 E3 ligase could be involved in vascular calcification of VSMCs by activating bone-forming signals during atherosclerosis progression PMID: 28029182
4. Our data suggest that the behavioral sensitivity to stress is dramatically reduced in FXS, opening new views on the impact of gene-environment interactions in this pathology. PMID: 27939692
5. Nedd4-1 is required for efficient internalization of major growth factor receptors involved in liver regeneration and their downstream mitogenic signaling. PMID: 28890072
6. NPM1-dependent nucleolar PIDDosome is a key initiator of the caspase-2 activation cascade. PMID: 28432080
7. these results suggest that Nedd4 suppresses colonic WNT signaling and tumor growth, at least in part, by suppressing the transcription factors LEF1 and YY1. PMID: 27086928
8. Findings indicate that reducing Nedd4 protein by 50% significantly impairs long-term potentiation and long-term memory thereby demonstrating an important role for Nedd4 in these processes. PMID: 26821291
9. Upregulation of long non-coding RNA PAPAS in response to hypoosmotic stress does not increase H4K20me3 because of Nedd4-dependent ubiquitinylation and proteasomal degradation of Suv4-20h2. PMID: 26904956
10. we propose that Nedd4 functions as a novel Pax7 regulator, which activity is temporally and spatially controlled to modulate the Pax7 protein levels and therefore satellite cell fate. PMID: 26304770
11. PDLIM7 is a bona fide skeletal muscle substrate of Nedd4-1. PMID: 26556890
12. show that absence of Nedd4 in pre-osteoblasts results in decreased cell proliferation and altered osteogenic differentiation PMID: 26681395
13. Nedd4 overexpression accelerates zebrafish embryonic growth through IRS-2 in vivo. PMID: 25879670
14. Nedd4 co-injection was found to have no affect on Gag- or Env-specific IFNgamma but had a trend of increased Gag-specific IL-6, IL-17A and TNFalpha that was not seen following Env stimulation. PMID: 24614057
15. NEDD4 is a previously unknown component of the CD40 signalling complex necessary for AKT activation. PMID: 25072696
16. Brucella infection inhibits macrophages apoptosis via Nedd4-dependent degradation of calpain2. PMID: 25258171
17. PTEN limits Nedd4-1 protein levels by modulating the activity of mTORC1, a protein complex that controls protein synthesis and cell growth. PMID: 25157163
18. Rsp5 and its mammalian homologue Nedd4 are important E3 ligases responsible for the increased ubiquitylation induced by heat stress. PMID: 25344756
19. This study is the first to show that a 50% reduction in Nedd4 levels is sufficient to produce significant gait defects in 6 month old mice PMID: 24280120
20. results identify Nedd4 as a novel binding partner for GluN2D and suggest a mechanism for the regulation of NMDA receptors that contain GluN2D subunits through ubiquitination-dependent downregulation PMID: 23639431
21. the expression profile of ubiquitin ligase Nedd4-2 during midcorticogenesis suggests that ubiquitin-mediated alterations in protein levels and protein turnover are required for neuron migration, positioning and maturation. PMID: 24036464
22. An essential role for the ubiquitin ligase Nedd4 in cranial neural crest cell development, is reported. PMID: 24080509
23. This suggests NEDD4-1 functions in conjunction with other post-translational mechanisms to regulate Thoc1 protein and THO activity. PMID: 23460917
24. The results demonstrate that Nedd4-1 plays an important role in mediating denervation-induced skeletal muscle atrophy in vivo. PMID: 23110050
25. Data suggest a role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins. PMID: 22096579
26. Dlg3 exerts a distinct function by recruiting the ubiquitin ligases Nedd4 and Nedd4-2 through its PPxY motifs. These interactions are required for Dlg3 monoubiquitination, apical membrane recruitment, and tight junction consolidation. PMID: 21920314
27. Nedd4-1 interferes with the chaperon role of Ca(v)beta at the endoplasmic reticulum/Golgi level to prevent the delivery of Ca(v) channels at the plasma membrane. PMID: 21220429
28. analysis of the interaction between the growth factor-binding protein GRB10 and the E3 ubiquitin ligase NEDD4 PMID: 20980250
29. Nedd4-1 and beta-arrestin-1 as key regulators of NHE1 ubiquitylation, endocytosis, and function. PMID: 20855896
30. NEDD4-1-mediated PTEN ubiquitination is crucial in the regulation of PI3K/Akt signaling by PTEN during the neuronal response to zinc, which may represent a common mechanism in neurodegeneration PMID: 20100827
31. Nedd4-1 is a suppressor of Tsp1 and increased levels of Tsp-1 in Nedd4-1 knock-out mice may have contributed to the developmental defect observed in the embryos PMID: 20026598
32. These results suggest a role for the Grb10/Nedd4 complex in regulating ubiquitination and stability of the IGF-IR, and they suggest that Grb10 serves as an adapter to form a bridge between Nedd4 and the IGF-IR. PMID: 12697834
33. Results provide evidence that Nedd4 and Nedd4-2 are likely to be key regulators of specific neuronal voltage-gated sodium channels in vivo. PMID: 15123669
34. ubiquinates APOBEC3G targeting the virus assembly site where APOBEC3G interacts with Gag and is packaged into HIV-1 particles in the absence of Vif PMID: 15581898
35. Our data indicate that DeltaNp63alpha is ubiquitinated in a Nedd4- and sumoylated in a Ubc9-dependent fashion, and that these modifications can regulate DeltaNp63alpha stability in the zebrafish ectoderm. PMID: 15908775
36. NEDD4-1 is a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. PMID: 17218260
37. These results indicate that Grb10 and Nedd4 play a critical role in mediating IGF-IR down-regulation by promoting ligand-dependent multiubiquitination of the IGF-IR, which is required for receptor internalization and regulates mitogenesis. PMID: 18286479
38. results fail to support a role for Nedd4-1 as the E3 ligase regulating PTEN stability and subcellular localization. PMID: 18562292
39. Nedd4 appears to positively control IGF-1 and insulin signaling partly through the regulation of Grb10 function. PMID: 18812566
40. We propose that Nedd4 and Itch ubiquitinate distinct target proteins in vivo. PMID: 18931680
41. these results demonstrate that Nedd4 is involved in regulating the formation and function of the neuromuscular junctions through non-cell autonomous mechanisms. PMID: 19345204
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亞細胞定位：

Cytoplasm. Cell membrane; Peripheral membrane protein.
組織特異性：

Brain.
數據庫鏈接：

KEGG: mmu:17999

STRING: 10090.ENSMUSP00000034740

UniGene: Mm.279923