Recombinant Mouse D-3-phosphoglycerate dehydrogenase (Phgdh)

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中文名稱：

Recombinant Mouse D-3-phosphoglycerate dehydrogenase (Phgdh)
品名簡稱：

Recombinant Mouse Phgdh protein
貨號：

CSB-EP717226MO
說明書：

MSDS Datasheet
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Phgdh
Uniprot No.：

Q61753
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

61.5 kDa
表達區域：

2-533aa
氨基酸序列

AFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWDRKKFMGTELNGKTLGILGLGRIGREVATRMQSFGMKTVGYDPIISPEVAASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDSTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEAMGTLMHAWAGSPKGTIQVVTQGTSLKNAGTCLSPAVIVGLLREASKQADVNLVNAKLLVKEAGLNVTTSHNPGVPGEQGSGECLLTVALAGAPYQAVGLVQGTTPMLQMLNGAVFRPEVPLRRGQPLLVFRAQPSDPGMLPTMIGLLAEAGVQLLSYQTSMVSDGEPWHVMGLSSLLPSLETWKQHVLEAFQFCF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Does not catalyze the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.
基因功能參考文獻：
1. These results indicate that the PHGDH gene expression, dictated by IL-2R signaling, is a crucial event for DNA synthesis during S phase of activated T cells. PMID: 24434753
2. Using transgenic mice expressing enhanced GFP under the Srr promoter and mice with targeted deletion of Srr or 3-Phosphoglycerate dehydrogenase, we demonstrate predominantly neuronal sources of d-serine dependent on astrocytic supply of l-serine. PMID: 23884950
3. Elevated levels of 4E-BP1 may be involved in a mechanism to arrest brain development in Phgdh knock out embryos. PMID: 23350942
4. Phgdh deletion reveals a pivotal role for L-serine biosynthesis in controlling the level of brain D-serine, an N-methyl-D-aspartate receptor co-agonist PMID: 20966073
5. Data suggest expression of Phgdh in endometrium is regulated by Hoxa10. PMID: 19778996
6. the Phgdh-dependent phosphorylated pathway is essential for normal embryonic development, especially for brain morphogenesis PMID: 14645240
7. the presence of cis-acting elements confer the cell type specificity of Phgdh transcription in the distal promoter region. PMID: 15139021
8. The cis-acting elements necessary for basal transcription of Phgdh are contained within the -196/+4 proximal sequence of the promoter, in which the conserved Sp1 recognition sites play an important role for basal promoter activity. PMID: 15256251
9. either Phgdh or ASCT1 is provided to each segment of renal tubules, suggesting that metabolic interplay mediated by L-serine biosynthesis and supply may exist in the kidney PMID: 17510490
10. Inactivation of Phgdh gene with changes in gene expression and associated regulatory networks resulting from serine deficiency are reported. PMID: 18228065
11. Results demonstrate injury-induced changes in Phgdh and ASCT1 expression. PMID: 18751891
12. normal cell cycle progression and subsequent neurogenesis of radial glia cells are severely impaired by serine deficiency PMID: 19114063
13. demonstrated expression of Phgdh was upregulated in subventricular zone; expression was selective to cells having features of astrocyte-like primary neural stem cells & their progeny; Phgdh expression was virtually absent in committed neuronal precursors PMID: 19429008
14. The mouse Phgdh gene spans approximately 27 kilobases in length and comprises 12 exons with 11 intervening introns and is mapped to chromosome 3, region F2-F3 by FISH. PMID: 15256251
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蛋白家族：

D-isomer specific 2-hydroxyacid dehydrogenase family
數據庫鏈接：

KEGG: mmu:236539

STRING: 10090.ENSMUSP00000064755

UniGene: Mm.16898