Recombinant Mouse Ammonium transporter Rh type B (Rhbg), partial

中文名稱：

小鼠Rhbg重組蛋白
貨號：

CSB-YP804406MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Rhbg重組蛋白
貨號：

CSB-EP804406MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Rhbg重組蛋白
貨號：

CSB-EP804406MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Rhbg重組蛋白
貨號：

CSB-BP804406MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Rhbg重組蛋白
貨號：

CSB-MP804406MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Rhbg
Uniprot No.：

Q8BUX5
別名：

Rhbg; Ammonium transporter Rh type B; Rhesus blood group family type B glycoprotein; Rh family type B glycoprotein; Rh type B glycoprotein
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Functions as a specific ammonium transporter.
基因功能參考文獻：
1. Utilizing a structural homology analysis to AmtB, we have identified residues in Rhbg that are implicated in ion transport function. We introduced mutations into these residues and evaluated their function using measurements of intracellular pH (pHi), surface pH (pHs), and whole cell currents (I) that can distinguish between NH3 and NH4+ signals. Our results identified critical amino acids that affect transport. PMID: 27681563
2. Rhbg is critically important in the renal response to metabolic acidosis. in mice with intact Rhbg expression, metabolic acidosis does not induce maximal changes in PEPCK, NHE-3, and GS expression despite the presence of persistent metabolic acidosis. PMID: 24338819
3. It was concluded that hypokalemia increases Rhbg expression in intercalated cells in the cortex and outer medulla and that intercalated cell Rhbg expression is necessary for the normal increase in renal ammonia excretion in response to hypokalemia. PMID: 23220726
4. These data indicate that transport of NH(4)(+) and methyl ammonium (+) by Rhbg is pH sensitive. PMID: 20810915
5. Rhbg in connecting segment and principal cells may contribute to renal ammonia excretion PMID: 20719974
6. Basolateral RhBG is expressed by perivenous hepatocytes, where it may mediate ammonium uptake PMID: 12730882
7. mIMCD-3 cells express a basolateral electroneutral NH4+/H+ exchange activity that may be mediated by RhBG. PMID: 15149971
8. Characterization of the role of Rhbg in ammonium ion transport using voltage clamp techniques. PMID: 15353405
9. concluded that Rhbg is not a critical determinant of NH(4)(+) excretion by the kidney and of NH(4)(+) detoxification by the liver in vivo PMID: 16077082
10. RhBG-mediated transport is an electroneutral process that is driven by the NH4+ concentration and the transmembrane H+ gradient, effectively exchanging NH4+ for H+ in a process that results in transport of net NH3. PMID: 16563829
11. Review. Animal studies show Rhbg has a role in transmembrane NH3 or NH4+ transport in epithelial cells. The absence of distal tubular acidosis or hyperammonemia in Rhbg KO mice raises new questions about the physiological significance of this protein. PMID: 16563831
12. Review. Rhbg is in distal nephron sites responsible for NH3 secretion and in perivenous hepatocyte basolateral plasma membrane. Cetabolic acidosis does not affect Rhbg, so it may be either an ammonia- sensor or mediate other roles. PMID: 16564723
13. Rhbg transport of NH4+ is electrogenic. Methylammonium is transported by Rhbg. NH4+ transport by Rhbg is saturated at high concentrations with Michaelis-Menten kinetics. PMID: 16580864
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亞細胞定位：

Basolateral cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein.
蛋白家族：

Ammonium transporter (TC 2.A.49) family, Rh subfamily
組織特異性：

Expressed in kidney by connecting segments and collecting tubules. Also expressed in liver by perivenous hepatocytes. Expressed in the forestomach and the fundus of the stomach. Expressed in duodenum, jejunum, ileum and colon at the level of villous (at p
數據庫鏈接：

KEGG: mmu:58176

STRING: 10090.ENSMUSP00000130767

UniGene: Mm.103777