Recombinant Human Ubiquitin-conjugating enzyme E2 D2 (UBE2D2)

中文名稱：

人UBE2D2重組蛋白
貨號(hào)：

CSB-YP025444HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人UBE2D2重組蛋白
貨號(hào)：

CSB-EP025444HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

人UBE2D2重組蛋白
貨號(hào)：

CSB-EP025444HU-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人UBE2D2重組蛋白
貨號(hào)：

CSB-BP025444HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

人UBE2D2重組蛋白
貨號(hào)：

CSB-MP025444HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

UBE2D2
Uniprot No.：

P62837
別名：

E2(17)KB2; OTTHUMP00000223475; OTTHUMP00000223476; OTTHUMP00000223477; OTTHUMP00000223478; OTTHUMP00000224375; PUBC 1; PUBC1; UB2D2_HUMAN; UBC 4; UBC 4/5; UBC4; UBC4/5; UBC4/5 homolog yeast; UBCH 5B; UBCH5B; UBE2D2; Ubiquitin carrier protein; Ubiquitin carrier protein D2; Ubiquitin conjugating enzyme E2 17 kDa 2; Ubiquitin conjugating enzyme E2 D2; Ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5); Ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog yeast); Ubiquitin conjugating enzyme E2D 2; Ubiquitin protein ligase D2; Ubiquitin-conjugating enzyme E2 D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17 kDa 2; Ubiquitin-protein ligase D2
種屬：

Homo sapiens (Human)
蛋白長度：

Full length protein
表達(dá)區(qū)域：

1-147
氨基酸序列

MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.
基因功能參考文獻(xiàn)：
1. Knockdown of UBE2D2 caused an increase in p53 protein levels, and knockdown of p53 attenuated not only cadmium-induced apoptosis, but also cadmium-induced apoptosis-related gene expression. PMID: 26912277
2. mutations having marked effects on function only minimally affect the intermolecular interactions between the AO7 RING and UbcH5B, establishing a high degree of complexity in activation through the RING-E2 interface. PMID: 26475854
3. together with UBE2L3 and UBE2N, synergistically contribute to Parkin-mediated mitophagy PMID: 24906799
4. Presented is the structure of the human dimeric RING domain from BIRC7 in complex with the E2 UbcH5B covalently linked to Ub. PMID: 22902369
5. determined the 2.2 A crystal structure of an intermediate of UbcH5b approximately ubiquitin (Ub) conjugate, which is assembled into an infinite spiral through the backside interaction. PMID: 20152160
6. role of WW3 and WW4 domains of Nedd4-2 in dopamine transporter ubiquitination was demonstrated; siRNA analysis demonstrated that this polyubiquitination is mediated by Nedd4-2 cooperation with UBE2D and UBE2L3 E2 ubiquitin-conjugating enzymes PMID: 20051513
7. Results describe the crystal structure of a complex between the HECT domain of NEDD4L and the E2 UbcH5B bearing a covalently linked Ub at its active site (UbcH5B approximately Ub). PMID: 20064473
8. Data show that binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. PMID: 15001359
9. UbcH5B/C are E2s for Mdm2, which contribute to the maintenance of low levels of p53 and Mdm2 in unstressed cells; inhibition of p53 ubiquitination and degradation by targeting UbcH5B/C is not sufficient to up-regulate p53 transcriptional activity. PMID: 15280377
10. Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation PMID: 18508924
11. UBE2D2 is required for GCMa ubiquitination and for association with the SCF(FBXW2) complex. PMID: 18703417
12. The results imply that the interaction specificity between c-Cbl, UbcH7 and UbcH5b is required but not sufficient for transfer of ubiquitin to potential targets. PMID: 18996392
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蛋白家族：

Ubiquitin-conjugating enzyme family
數(shù)據(jù)庫鏈接：

HGNC: 12475

OMIM: 602962

KEGG: hsa:7322

STRING: 9606.ENSP00000381717

UniGene: Hs.108332