Recombinant Human TNF receptor-associated factor 4 (TRAF4)

中文名稱：

人TRAF4重組蛋白
貨號：

CSB-YP024152HU
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人TRAF4重組蛋白
貨號：

CSB-EP024152HU
規(guī)格：
來源：

E.coli
其他：

中文名稱：

人TRAF4重組蛋白
貨號：

CSB-EP024152HU-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人TRAF4重組蛋白
貨號：

CSB-BP024152HU
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

人TRAF4重組蛋白
貨號：

CSB-MP024152HU
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

TRAF4
Uniprot No.：

Q9BUZ4
別名：

CART 1; CART1; cysteine rich domain associated with ring and TRAF domain ; Cysteine rich domain associated with RING and Traf domains protein 1; Cysteine-rich domain associated with RING and Traf domains protein 1; Malignant 62; Metastatic lymph node gene 62 protein; MLN 62; MLN62; RING finger protein 83; RNF 83; RNF83; TNF receptor associated factor 4 ; TNF receptor-associated factor 4; TRAF 4; Traf4; TRAF4_HUMAN; tumor necrosis receptor-associated factor 4A
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達區(qū)域：

1-470
氨基酸序列

MPGFDYKFLE KPKRRLLCPL CGKPMREPVQ VSTCGHRFCD TCLQEFLSEG VFKCPEDQLP LDYAKIYPDP ELEVQVLGLP IRCIHSEEGC RWSGPLRHLQ GHLNTCSFNV IPCPNRCPMK LSRRDLPAHL QHDCPKRRLK CEFCGCDFSG EAYESHEGMC PQESVYCENK CGARMMRRLL AQHATSECPK RTQPCTYCTK EFVFDTIQSH QYQCPRLPVA CPNQCGVGTV AREDLPGHLK DSCNTALVLC PFKDSGCKHR CPKLAMARHV EESVKPHLAM MCALVSRQRQ ELQELRRELE ELSVGSDGVL IWKIGSYGRR LQEAKAKPNL ECFSPAFYTH KYGYKLQVSA FLNGNGSGEG THLSLYIRVL PGAFDNLLEW PFARRVTFSL LDQSDPGLAK PQHVTETFHP DPNWKNFQKP GTWRGSLDES SLGFGYPKFI SHQDIRKRNY VRDDAVFIRA AVELPRKILS
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Adapter protein with E3 ligase activity that is involved in many diverse biological processes including cell proliferation, migration, differentiation, DNA repair, platelet activation or apoptosis. Promotes EGFR-mediated signaling by facilitating the dimerization of EGFR and downstream AKT activation thereby promoting cell proliferation. Ubiquitinates SMURF2 through 'Lys-48'-linked ubiquitin chain leading to SMURF2 degradation through the proteasome and subsequently osteogenic differentiation. Promotes 'Lys-63'-mediated ubiquitination of CHK1 which in turn activates cell cycle arrest and activation of DNA repair. In addition, promotes an atypical 'Lys-29'-linked ubiquitination at the C-terminal end of IRS1 which is crucial for insulin-like growth factor (IGF) signal transduction. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Inhibits adipogenic differentiation by activating pyruvate kinase PKM activity and subsequently the beta-catenin signaling pathway.
基因功能參考文獻：
1. circRNA MTO1 (hsa-circRNA-007874) interacted with TRAF4, and sequester it from activating Eg5 translation, thereby inhibiting the Eg5 protein level inhibiting Eg5-mediated cell viability and promoting monastrol chemosensitivity in breast cancer cells. PMID: 30015883
2. It was identified that a high level of TRAF4 facilitated the invasiveness of intrahepatic cholangiocarcinoma (ICC)cells via the activation of AKT signaling. The overexpression of TRAF4 may be a prognostic biomarker and candidate therapeutic target for patients with ICC. PMID: 29749456
3. expression of miR-519d-3p is down-regulated in prostate cancer cells. Overexpression of miR-519d-3p can inhibit the proliferation of prostate cancer cells. The possible mechanism is that miR-519d-3p inhibits the expression of TRAF4. PMID: 29595452
4. TRAF4 knockdown significantly inhibited proliferation, invasion and metastasis in the xenograft nude mouse model, possibly involving in the interaction with RSK4. PMID: 29684350
5. Study demonstrated that TRAF4 was involved in promoting HCC cell migration and invasion. The process was induced by the EMT through activation of the PI3K/Akt signaling pathway. PMID: 28256185
6. increased TRAF4 expression in ankylosing spondylitis mesenchymal stem cells impaired LPS-induced autophagy, potentially by inhibiting the phosphorylation of Beclin-1. PMID: 28604663
7. TRAF4 expression is higher in mastocytosis patients with food hypersensitivity in their medical history, while B3GAT1 expression is lower in mastocytosis patients with insect venom allergy in history PMID: 27086366
8. Determined the crystal structure of TRAF domain of TRAF4 (residues 292-466) at 2.60 A resolution by X-ray crystallography method. The trimericly assembled TRAF4 resembles a mushroom shape, containing a super helical "stalk" which is made of three right-handed intertwined alpha helixes and a C-terminal "cap", which is divided at residue L302 as a boundary. PMID: 23982741
9. TRAF4 may promote oral squamous carcinoma cell growth, invasion and migration by Wnt/beta-catenin pathway. PMID: 26617938
10. There was a significant positive correlation between TRAF2 and TRAF4 expression levels in malignant pleural effusion cells PMID: 26331901
11. The TRAF4-ERK5 is a dominant pathway in human skin squamous cell carcinoma. PMID: 26347473
12. Data showed that TRAF4 was highly expressed in non-small cell lung cancer (NSCLC) cells, and miR-370 overexpression significantly inhibited its expression. These results suggest that TRAF4 serves as an oncogene in NSCLC. PMID: 25976502
13. these results suggest that TRAF4 promoted colon cancer cell growth and invasion PMID: 25973026
14. Expression of tumor necrosis factor receptor-assicated factor 4 correlates with expression of Girdin and promotes nuclear translocation of Girdin in breast cancer PMID: 25591657
15. p70s6k/S6 signaling pathway played an important role in the promoting function of TRAF4 on cell proliferation. This work suggests a new direction for understanding the oncogenic function of TRAF4 in breast cancer. PMID: 25738361
16. Recent data implicates TRAF4 in carcinogenesis. The molecular mechanism addressing TRAF4 to tight junctions involves lipid binding by the TRAF domain. Review. PMID: 25840457
17. TRAF4 upregulated PRMT5 expression, which occurred predominantly in the nucleus, on which TRAF4 promotion of cell proliferation in breast cancer is mainly dependent. PMID: 25704480
18. Data indicate that TRAF4 is specifically overexpressed in human breast cancer cells and plays an important role in tumorigenesis of breast cancer through direct interaction and activation of Akt. PMID: 24993240
19. Results identified beta-catenin as a TRAF4-binding protein, TRF4 enhanced expression of beta-catenin, and mediated its translocation from the cytoplasm to the nucleus, thereby facilitating activation of the Wnt signaling pathway in breast cancer. PMID: 24990246
20. promotes tumorigenesis in osteosarcoma cell lines via Akt signaling PMID: 25700355
21. review highlights TGF-beta-induced SMAD-dependent signaling and non-SMAD signaling as the major pathways regulated by TRAF4 involved in breast cancer metastasis PMID: 25249198
22. Data indicate that tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) regulated osteosarcoma cell growth in vitro and in vivo, suggesting a candidate molecular target for osteosarcoma prevention and therapy. PMID: 25270078
23. As a first step towards elucidating the molecular mechanisms of the TRAF4-mediated signalling pathway, the first crystal structure of the human TRAF4 TRAF domain with a coiled-coil domain is reported at 2.3 A resolution. PMID: 24419373
24. possesses a novel phosphoinositide (PIP)-binding domain crucial for its recruitment to tight junctions PMID: 24311986
25. our results suggest that the tumor suppressor microRNA, miR-29a, is one of the regulators of expression in metastatic prostate cancer. PMID: 24100420
26. TRAF4 attenuation impaired glucose metabolism by inhibiting expression of Glut1 and HK2 mediated by the Akt pathway. PMID: 24154876
27. TRAF4 regulates the TGF-beta pathway and is a key determinant in breast cancer pathogenesis. PMID: 23973329
28. The short form of the TRAF4 TRAF domain was purified to homogeneity and crystallized. PMID: 23908043
29. Data indicate that Smad ubiquitin regulatory factor Smurf1 regulates cell migration through ubiquitination of tumor necrosis factor receptor-associated factor 4 (TRAF4). PMID: 23760265
30. Data iindicate that TRAF2 affects the localization and function of TRAF4 in breast cancer cell lines. PMID: 23743189
31. The results indicate that TRAF4 and TRAF6 are overexpressed in IBD. TRAF4 and TRAF6 play different roles in the pathogenesis of IBD PMID: 23431243
32. Primary cells from TRAF4-deficient mice display markedly enhanced IL-17-activated signaling pathways and induction of chemokine mRNA/ PMID: 22649194
33. Like IKKalpha, TRAF4 is atypical within its family because it is the only TRAF family member to negatively regulate innate immune signaling. IKKalpha's phosphorylation of serine-426 on TRAF4 was required for this negative regulation. PMID: 22547678
34. Identify TRAF4 as a novel binding partner for GPIb-IX-V and GPVI in human platelets. PMID: 20946164
35. A novel motif in the Crohn's disease susceptibility protein, NOD2, allows TRAF4 to down-regulate innate immune responses. PMID: 21097508
36. Involvement in oxidative activation of c-Jun N-terminal kinase PMID: 12023963
37. activation of the NF-kappaB pathway is involved in up-regulation of TRAF4 in T-cells PMID: 12354113
38. TRAF4 may play a role in p53-mediated proapoptotic signaling in the response to cellular stress PMID: 12788948
39. TNF-receptor activation leads to activation of the p70S6K; TRAF4 is a mediator in this TNF-induced signaling pathway; and TRAF4 inhibits Fas-induced apoptosis PMID: 12801526
40. MEKK4 is the MAPK kinase kinase for TRAF4 regulation of the JNK pathway PMID: 16157600
41. TRAF4 gene amplification is one of the mechanisms responsible for TRAF4 protein overexpression in human cancers PMID: 16799635
42. The nuclear expression of TRAF4 in breast carcinoma is suppressed, and correlated to the invasive ability of breast cancer. PMID: 17927880
43. Disrupting endogenous p63 expression downregulated TRAF4 mRNA & protein in SCCHN cells. Endogenous p63 bound the TRAF4 promoter in vivo. TAp63 is the most active transactivator of TRAF4. TRAF4 is associated with differentiation of SCCHN cells. PMID: 18087216
44. Apart from being a well replicated risk factor for RA, TRAF1/C5 also appears to be a risk factor for the rheumatoid factor-negative polyarthritis subtype of JIA and seems to be associated with subtypes of JIA characterised by a polyarticular course. PMID: 18593758
45. TRAF4 is one of the emerging TJ-dependent signaling pathways that responds to cell polarity by regulating the cell proliferation/differentiation balance, and subsequently epithelium homeostasis. PMID: 18953416
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亞細胞定位：

Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Cell junction, tight junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton.
蛋白家族：

TNF receptor-associated factor family, B subfamily
組織特異性：

Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
數(shù)據(jù)庫鏈接：

HGNC: 12034

OMIM: 602464

KEGG: hsa:9618

STRING: 9606.ENSP00000262395

UniGene: Hs.8375