Recombinant Human Selenoprotein P (SELENOP) (U59S,U300S,U318S,U330S,U345S,U352S,U367S,U369S,U376S,U378S)

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中文名稱：

人SELENOP重組蛋白
品名簡稱：

Recombinant Human SELENOP protein (Mutant type)
貨號：

CSB-EP021018HU
說明書：

MSDS Datasheet
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

SELENOP
Uniprot No.：

P49908
別名：

Selenoprotein P; Selenoprotein P plasma 1; Selp; SeP; Sepp1; SEPP1_HUMAN
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

44.6kDa
表達(dá)區(qū)域：

20-381aa(U59S, U300S, U318S, U330S, U345S, U352S, U367S, U369S, U376S, U378S)
氨基酸序列

ESQDQSSLCKQPPAWSIRDQDPMLNSNGSVTVVALLQASSYLCILQASKLEDLRVKLKKEGYSNISYIVVNHQGISSRLKYTHLKNKVSEHIPVYQQEENQTDVWTLLNGSKDDFLIYDRCGRLVYHLGLPFSFLTFPYVEEAIKIAYCEKKCGNCSLTTLKDEDFCKRVSLATVDKTVETPSPHYHHEHHHNHGHQHLGSSELSENQQPGAPNAPTHPAPPGLHHHHKHKGQHRQGHPENRDMPASEDLQDLQKKLCRKRCINQLLCKLPTDSELAPRSSCCHCRHLIFEKTGSAITSQCKENLPSLCSSQGLRAEENITESCQSRLPPAASQISQQLIPTEASASSRSKNQAKKSESPSN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

A construct encoding NH₂-terminally 6xHis-tagged human selenoprotein P (SEPP1) was expressed in the E.coli cells. The resulting protein is the recombinant full-length of mature SEPP1 protein containing amino acid sequence 20-381 of mouse SEPP1. The purity of this SEPP1 protein is greater than 90% determined by SDS-PAGE. A molecular mass band range from 40 kDa was presented on the SDS-PAGE gel under reducing conditions. In-stock SEPP1 proteins are offered now. In addition to being used to immunize animals for specific antibody synthesis, this recombinant SEPP1 protein may also find use int he studies of selenium-metabolism or SEPP1-related diseases.

SEPP1 is a major selenium-containing protein in human plasma and is primarily expressed in the liver and secreted into the extracellular fluid. As a selenium-transporter, SEPP1 sustains antioxidative selenoenzymes in several tissues such as the brain and testis and plays an important role in selenium-metabolism and antioxidative defense. The reduction of SEPP1 and other selenoproteins leads to various dysfunctions associated with oxidative stress. Recent studies have shown that overexpressed SEPP1 exacerbates glucose metabolism and promotes type II diabetes.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.
基因功能參考文獻(xiàn)：
1. When rs13154178 gene polymorphism was compared with AA homozygous individuals, fasting blood glucose levels were significantly higher in carriers of either polymorphism than in those with no polymorphism. We suggest rs13154178 gene polymorphism may lead to gestational diabetes mellitus in the Turkish society. PMID: 29648467
2. preliminary result indicated the beneficial effect of serine on the expression of SelP and GPx, which suggested that it might be a candidate for combined selenium supplement. PMID: 26944060
3. Genetic variations in selenoprotein genes modulated both GPX1 and SELENOP selenoprotein gene expression and global gene expression in response to Brazil nut supplementation. PMID: 28696394
4. SeP was higher in individuals who were overweight/obese, and associated with insulin resistance by HOMA-IR and by clamp, but not independently of BMI. PMID: 27524654
5. We have demonstrated a significant decrease in circulating SeP levels according to MetS status in patients with documented cardiovascular disease. PMID: 28190280
6. Results indicate a diversity of RNA elements conducting multiple occurrences of UGA redefinition to control the synthesis of full-length and truncated selenoprotein P (SELENOP) isoforms. PMID: 29069514
7. Biomarkers of Se status in clinical research need to be measured by validated assays in order to avoid erroneous data and incorrect interpretations, especially when analyzing young women. The Se content of circulating SELENOP differs between individuals and may provide some important diagnostic information on Se metabolism and status PMID: 28064116
8. Study suggests the 3' UTR structural elements (SECIS) in Sepp1 functions with site specificity and further illustrates how mRNA processing may produce transcripts with altered coding potential to produce diversity in selenoprotein isoforms. PMID: 27881738
9. Increased amounts of circulating SeP predicted the ineffectiveness of training on endurance capacity in humans. PMID: 28263310
10. Results indicate that the 2 genetic variants of rs7579 and rs230813 in SEPP1 may not play a role in the pathogenesis of preeclampsia in Chinese Han Women. PMID: 28700468
11. erum microRNA-7 and selenoprotein P appear to be potential non-invasive diagnostic markers for hepatocellular carcinoma. Moreover, the results suggest that selenium could be used as an anticancer therapy for hepatocellular carcinoma by affecting both microRNA-7 and selenoprotein P PMID: 28459371
12. The selenium levels in the tissues were correlated to the genotype of the SELENOP selenium carrier protein, but not to other proteins whose levels have been reported to be responsive to selenium availability, including GPX1, SELENOF and SBP1. PMID: 27908419
13. GPX1 and SEPP1 Single Nucleotide Polymorphism were not associated with any changes in the expression of related genes. GPX1 was shown to modulate the expression of unrelated target, SEP15, upon Se supplementation, both alone and in combination with SEPP1. PMID: 26658762
14. Data show that the cell proliferative ability was inhibited in the cells overexpressing selenoprotein P, plasma 1 (SEPP1), and the colony forming ability of The cell evidently decreased. PMID: 27371843
15. Selenoprotein P concentrations are not elevated in women with gestational diabetes mellitus but are associated with BMI and HDL cholesterol. PMID: 26514640
16. Studied gene-specific regulation of hepatic selenoprotein expression by interleukin-6; by testing human hepatocytes in culture, found IL-6 reduced the concentrations of SePP mRNA and secreted SePP in a dose-dependent manner. PMID: 26399395
17. Impaired renal function is positively correlated with serum concentrations of SePP. PMID: 26348725
18. women who carry the SEPP1 rs3877899 A allele are better able to maintain selenium status during pregnancy, and their GPx3 activity increases more with supplementation, which suggests better protection from low selenium status. PMID: 26675765
19. this study provide preliminary genetic evidence that the SEPP1 functional variants contribute to the AAA risk. PMID: 25395084
20. Elevated SeP concentrations are independently associated with a reduced risk of MetS in children PMID: 24638201
21. These results demonstrate that SEPP1 represents a previously unrecognized regulator of Polychlorinated biphenyl-induced biological effects. PMID: 23770201
22. Selenium, selenoenzymes, oxidative stress and risk of neoplastic progression from Barrett's esophagus: results from biomarkers and genetic variants. PMID: 22715394
23. These results demonstrated that SelP interacts with tubulin, alpha 1a (TUBA1A). PMID: 24914767
24. data suggest that SEPP1 alters breast cancer risk among women with higher levels of NA ancestry PMID: 24278290
25. The histidine-rich domain of selenoprotein P is capable of binding Cu ions in both oxidation states of Cu(+) and Cu(2+) with high affinity and of modulating Cu(+) and Cu(2+)-mediated ABETA aggregation, reactive oxygen species production, neurotoxicity. PMID: 24437729
26. Data suggest that plasma SEPP1 level can be up-regulated by dietary factors; here, significantly higher plasma SEPP1 is observed in women at risk of pre-eclampsia treated with dietary supplement of selenium-enriched yeast started in first trimester. PMID: 24708917
27. SEPP1 rs7579 was found to be associated with lower risk of advanced prostate cancer. PMID: 24563517
28. This study demonistrated that Sepp1 in midbrain was expressed in neurons of the substantia nigra (SN), and expression was concentrated within the centers of Lewy bodies, the pathological hallmark of Parkinson disease PMID: 23268326
29. The data provide evidence that single nucleotide polymorphisms in SEPP1 and GPX1 modulate risk of breast cancer. PMID: 24039907
30. SePP is not only involved in Selenium transport to testes supporting GPX4 biosynthesis but it also becomes secreted into seminal plasma. PMID: 24361887
31. metformin decreases binding of FoxO3a, a direct target of AMPK, to the SEPP1 promoter. PMID: 24257750
32. Sepp1 secreted from epithelial cells may support the intestinal immune system by providing immune cells (including plasma cells) with selenium PMID: 24157689
33. We speculate that there could be a feedback regulation between hepatic Sepp1 and pancreatic insulin and that increased circulating Sepp1 might be the result rather than the cause of abnormal glucose metabolism.[Review] PMID: 23760059
34. Genetic variants in selenoprotein P plasma 1 gene (SEPP1) are associated with fasting insulin and first phase insulin response in Hispanics PMID: 24161883
35. SEPP1 genetic variation was associated with prostate cancer incidence; replication of these results in an independent dataset is necessary. PMID: 23129481
36. Higher tumor grade and tumor stage at diagnosis correlated to lower SePP and Se concentrations. PMID: 23056383
37. hepatocellular SAM-dependent protein methylation is required for both SEPP1 and gluconeogenic enzyme expression and that inhibition of protein arginine methylation might provide a route to therapeutic interventions in type II diabetes. PMID: 22932905
38. Circulating selenoprotein P levels were positively correlated with fasting plasma glucose and negatively associated with both total and high-molecular adiponectin in type 2 diabetics. PMID: 22496878
39. Circulating SeP concentrations were elevated in patients with glucose metabolism dysregulation and were related to various cardiometabolic parameters including insulin resistance, inflammation, and atherosclerosis. PMID: 21677040
40. The glucocorticoid receptor inhibits selenoprotein P transactivation via response elements; the finding provides insight into a potential mechanism of selenium redistribution during development. PMID: 19513589
41. Selenoprotein P levels in prostate cells determine basal reactive-oxygen-species levels and H2O2-induced cytotoxicity. Deregulation of selenoprotein P during prostate carcinogenesis may increase free radicals, thus promoting tumor development. PMID: 21456065
42. Using serial analysis of gene expression and DNA chip methods, the study found that hepatic SeP mRNA levels correlated with insulin resistance in humans. PMID: 21035759
43. tear SeP is a key molecule to protect the ocular surface cells against environmental oxidative stress PMID: 20360971
44. Inflammation of the intestinal mucosa causes a decline in locally produced selenoprotein P in the colon that eventually may contribute to the emergence of inflammatory bowel disease-related colorectal cancer. PMID: 20542496
45. The present results show no significant difference in genotype and allele distribution of SNP r25191g/a between individuals with Kashin-Beck disease and controls. PMID: 20565998
46. These findings suggest an association between the individual selenoprotein concentration and the presence of diabetes. PMID: 20875901
47. Our findings demonstrate that Selenoprotein P protects neuronal cells from amyloid beta-induced toxicity, suggesting a neuroprotective role for SelP in preventing neurodegenerative disorders. PMID: 20521393
48. Early decrease in Sel-P plasma concentrations was specifically observed in septic shock and was similar in SIRS patients whereas GPx activity remained unchanged. PMID: 19779296
49. A DNA-repeat structure within the promoter contains a functionally relevant polymorphism and is genetically unstable under conditions of mismatch repair deficiency PMID: 12173025
50. selenoprotein P functions as a Se-supply protein, delivering Se to the cells PMID: 12423375
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Selenoprotein P family
組織特異性：

Made in the liver and heart and secreted into the plasma. It is also found in the kidney.
數(shù)據(jù)庫鏈接：

HGNC: 10751

OMIM: 601484

KEGG: hsa:6414

STRING: 9606.ENSP00000420939

UniGene: Hs.275775