Recombinant Human Neurofilament light polypeptide (NEFL)

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中文名稱：

人NEFL重組蛋白
貨號：

CSB-EP015688HU
說明書：

MSDS Datasheet
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP015688HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NEFL.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP015688HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NEFL.
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產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

NEFL
Uniprot No.：

P07196
別名：

NEFL; NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

81.4kDa
表達區域：

2-543aa
氨基酸序列

SSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

神經絲輕多肽（NEFL）是神經絲家族中的一個亞單位，是神經元細胞中的主要結構蛋白之一。NEFL主要存在于長軸突的軸絲中，其主要功能是提供細胞結構支持，并參與神經元信號傳導。NEFL的異常表達與多種神經系統疾病，尤其是與神經元退行性疾病如運動神經元病相關。

華美生物提供的重組人NEFL蛋白系由大腸桿菌表達系統表達而成的全長重組蛋白，表達區域為2-543aa。這一產品在神經科學、神經疾病研究以及神經生物學方面具有廣泛的應用。

NEFL作為神經元細胞中的重要結構蛋白，其研究對于理解神經元結構、神經元的穩定性以及神經元的功能具有關鍵的意義?？茖W家可以利用這一蛋白進行神經細胞骨架的研究，深入了解NEFL在神經元發育和維持中的生物學功能。此外，NEFL的異常表達與神經退行性疾病密切相關，因此對其進行深入研究有助于揭示神經系統疾病的潛在機制。
Datasheet & COA：

Please contact us to get it.

產品評價

平均分:

5.0分 - 1 個評價

實驗應用：結合實驗/蛋白間互作, 體外酶活性檢測

產品評價：我購買的是原核表達的重組蛋白，收到后我做ELISA檢測蛋白結合活性，一抗用anti-hNF-L或mIgG1，二抗用HRP標記的Anti-Mouse IgG，結果蛋白的結合活性非常好，EC50為0.05ug/ml，后續考慮繼續大量訂購。

By 劉老師

問答及客戶評論

■ 常見問題解答

I'm experiencing difficulties with measuring protein using nanodrop A280.?I was wondering if you have tested the protein concentration of the Recombinant 68kDa filament protein (CSB-EP015688HU) using this technique?

We use the Bradford method to detect its concentration. Glycerol has a strong interference to the NanoDrop 2000c detection, even with a final concentration of 10% glycerol (you can refer to this report: http://www.sislab.info/NPhP_compa_ND.pdf ). A280 can be measured before adding glycerol. After adding glycerol, use the measured value before doing a conversion. If the protein you ordered is in liquid form, it should have some glycerol added, so it will encounter difficulties. It is recommended that you can try another concentration detection method to test again, such as the Bradford method.

Could?you?please?help?to?check?if?isoelectric?point?information?of?product?CSB-EP015688HU is?available?

CSB-EP015688HU Theoretical pI: 4.89

Does CSB-EP015688HU contain imidazol?

We have performed dialysis to change the buffer, and the imidazole is removed, but the final situation is not tested, so there is no guarantee that there will be no imidazole at all.

Is it possible to provide a small size as 5ug for CSB-EP015688HU? And do you also provide this protein from Mouse or Rat species?

Yes, we could offer a small size of 5ug as a free sample for you to test as long as you can share the test report with us, please provide your shipping information and application for this protein.
Yes, we can offer this protein from Mouse and Rat species. Please go to the following product pages to check them.

https://www.cusabio.com/Recombinant-Protein/Recombinant-Mouse-Neurofilament-light-polypeptideNefl-Yeast-19605.html
Recombinant Mouse Neurofilament light polypeptide(Nefl)
CSB-YP015688MO >> Yeast
CSB-EP015688MO >> E.coli
CSB-BP015688MO >> Baculovirus
CSB-MP015688MO >> Mammalian cell

https://www.cusabio.com/Recombinant-Protein/Recombinant-Rat-Neurofilament-light-polypeptide-Nefl--5739.html
Recombinant Rat Neurofilament light polypeptide(Nefl)
CSB-YP015688RA >> Yeast
CSB-EP015688RA >> E.coli
CSB-BP015688RA >> Baculovirus
CSB-MP015688RA >> Mammalian cell

We order this protein CSB-EP015688HU several times and are satisfied with the test result, is there any discount for bulk orders?

Yes, we can offer an additional discount for bulk orders, please let us know your demanded quantity and your requirement for the package and shipping format.

Concerning the Recombinant Human Neurofilament light polypeptide, I have a question about the tag(s) and the possibility to obtain the protein with a free N-terminus ( if possible also C-terminal free). From the SUMO-tag, There exist the possibility to remove by protease at the end of the purification process. Can you check with??Cusabio’s R&D people which possibilities exist?

The protein of CSB-EP015688HU that we currently developed is N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged.
If you need to remove the tag or tag-free protein, please communicate with us in advance. For the C-terminal tag, we can definitely guarantee to remove, however, for the N-terminal tag, we can try enzyme digestion, but we can't guarantee 100% successfully. . Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge an extra cost.
If we fail in removing the tag, we won’t charge for any extra cost and remark this information in the datasheet as follows “Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.

Has your protein CSB-EP015688HU already passed the ELISA test?

The proteins that we currently have in stock all passed the ELISA verification.

We are looking for a NEFL Recombinant protein (Human) that is suitable to use as a standard for an ELISA. Is the CSB-EP015688 suitable? If yes, would be able to recommend an antibody pair that has been optimized in ELISA for this protein?

There are many ELISA experimental factors, which are related to your experimental platform and technology, and each experimental stage may affect the experimental results. It is recommended that you use the corresponding expression system to express protein and antibody detection, and first purchase small specifications for testing. Sorry, at the same time, we have no corresponding Antibody pair recommendation.

Can?you?also?confirm?that?these?sources?are?carrier?protein?free?

All of our proteins are carrier-free.

For this protein CSB-EP015688HU, is there any affinity tag on the protein?

Yes, there is an affinity tag (His-sumo tag) on the protein.

Is this protein soluble?

We offer five expression systems: E. coli, Yeast, Baculovirus, Mammalian cell, and Cell-Free (in vitro E.coli). Among them, yeast only has supernatant expression, and the protein expressed in the supernatant must be a soluble protein.
The E. coli, Baculovirus, Mammalian cell, and Cell-Free (in vitro E.coli) have both supernatant expression and inclusion body expression, as long as the protein expressed in the supernatant, it must be soluble, if it is the protein expressed by in the inclusion body, we will also take a variety of methods to refold it to finally ensure that all the proteins we provide are soluble.
So all the proteins we offer are soluble.

How to reconstitute this protein CSB-EP015688HU?

Please use deionized sterile water to reconstitute proteins. (Dissolve completely. The concentration could depend on your experiment demand.)
We suggest that the concentration is not lower than 0.1mg/ml. Proteins will be unstable if they're too diluted. Generally, we recommend 0.1-1.0mg/ml concentration. If you have special requirements for the concentration, you could dilute the protein according to their experiment demand.
We haven’t studied whether the optimal concentration range will vary among different hosts such as e.coli, yeast, mammalian, or baculovirus, so we can’t provide usable advice.
If you need to use it immediately after dissolving one protein, you don't need to add glycerol. If you won't use the protein in short term, we suggest adding some glycerol for storage. Reconstituting a suitable amount of protein and store the remaining protein in lyophilized powder is recommended. You could remark "The lyophilized powder should be packed into multiple vials" when placing orders. The amount of lyophilized powder in each vial will depend on your experiment demand.
In principle, the final concentration of glycerol from 20% to 50% will be OK. Our default final concentration of glycerol is 50%. You could use it as a reference.

Have you validated the activity of this protein? If not, how to express a protein with bioactivity?

This protein is not included in our activity verification plan currently, we haven’t tested the activity of this protein yet, you need to empirically determine if it has protein activity.
A protein from the expression to the establishment of the activity test system to complete the activity verification requires a lot of cost and time investment. We are sorry that we have not tested it and do not guarantee activity. In theory, as what we provide is the full length of the mature protein, and we adopt affinity chromatography for purification, which is purified under a very mild condition, it's very likely to be active. We recommend that you can order a small size to have a try.
For gaining a protein with bioactivity, you should choose a right expression system, a suitable expression vector, an appropriate purification method, and a validation experiment. You can learn more from this link: https://www.cusabio.com/c-20275.html.
We will refer to the relevant literature on the preparation method of the active protein on the market, or if you have consulted the relevant literature and hope that we can prepare according to the methods in the literature, we will ensure the activity to the maximum according to the preparation methods mentioned in the literature.

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靶點詳情

功能：

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
基因功能參考文獻：
1. NF-L may be involved in severity of neuronal injury following traumatic brain injury PMID: 27819296
2. CSF neurofilament light chain protein is an accurate marker for distinguishing Alzheimer's disease patients from healthy controls. PMID: 28527630
3. Cerebrospinal fluid neurofilament protein light is a useful tool for determining disease intensity in frontotemporal dementia and motor neuron disease patients. PMID: 28631955
4. Our results suggest that plasma NFL levels may not be a useful biomarker for the diagnosis of prodromal and dementia stages of AD. PMID: 28428015
5. In this Chinese Han population a novel Charcot-Marie-Tooth disease-associated gene mutations of NEFL (c.280C>T) was discovered. PMID: 27862672
6. We conclude that low BDNF and high LCN2 and NF-L levels are associated with Multiple Sclerosis (MS) pathogenesis, and high IGFBP1level is a biomarker for female MS only, suggesting different MS progression pathways between the sexes. LCN2 is a candidate predictor of response to natalizumab treatment, and NF-L is a candidate predictor of clinically isolated syndrome's (CIS) conversion into MS. PMID: 29108879
7. This study showed tat Serum NfL concentration is increased in familial Alzheimer disease prior to symptom onset and correlates with measures of disease stage and severity. Serum NfL may thus be a feasible biomarker of early AD-related neurodegeneration. PMID: 29070659
8. Results show that both alphaS and NFL can be phosphorylated by CKII, PLK2 and PLK3, but Ser129 in alphaS is a preferential site for PLK2 and PLK3, demonstrating higher phosphorylation efficiency. Comparatively, CKII preferentially phosphorylates Ser473 in NFL and this site can be phosphorylated by PLK1, 2 and 3, but these enzymes prefer to modify other sites within NFL. PMID: 27503460
9. fL is a weak independent risk factor in clinically isolated syndromes for conversion to multiple sclerosis. Its role as an axonal damage biomarker may be more relevant as suggested by its association with medium-term brain volume changes. PMID: 27521440
10. Results from 2 independent prospective cohort studies show that serum NFL is a sensitive and dynamic biomarker for axonal injury in concussive traumatic brain injury. The marker should be useful to detect and monitor CNS injury in concussion PMID: 28404801
11. Findings support the potential value of serum NfL as a marker of neuroaxonal injury in early multiple sclerosis. Its reduction over time could represent regression to the mean, or a possible treatment effect of IFN-beta-1a. Association with whole brain atrophy and formation of acute white matter lesions has implications to use serum NfL as a biomarker of the overall consequences of brain damage and ongoing disease acti... PMID: 28148632
12. Higher serum NfL concentrations are associated with more rapid brain atrophy and may therefore reflect disease intensity in dementia (FTD). Because blood sampling is less invasive and has better patient acceptability than lumbar puncture, serum NfL may provide important prognostic information and prove to be a useful outcome measure for clinical trials in FTD. PMID: 27581216
13. We conclude that the NEFL N98S mutation is associated with a dominant intermediate Charcot-Marie-Tooth disease phenotype characterized by early-onset sensorimotor neuropathy delaying motor milestones, which may evolve into a severe and complex clinical picture including cerebellar ataxia. PMID: 26645395
14. Plasma Concentration of the Neurofilament Light Protein (NFL) is a Biomarker of CNS Injury in HIV Infection PMID: 26870824
15. The results showed an important role for miR-25 in regulating NEFL expression in Glioblastoma multiforme. PMID: 26209061
16. Finally, we demonstrated that NEFL inhibited the NF-kappaB pathway, thereby suppressing the expression of uPA and decreasing NSCLC invasiveness and migration. PMID: 26801564
17. Cerebrospinal fluid NFL concentration is increased by the early clinical stage of Alzheimer's Disease. PMID: 26524180
18. The miR-381-NEFL axis is important for temozolomide resistance in glioblastoma multiforme. PMID: 25605243
19. The results od this study concluded that NEFL E396K mutation may manifest with a novel DI-CMT phenotype, characterized by simultaneous involvement of the peripheral and central nervous system. PMID: 25877835
20. Suggest monitoring the serum level of antibodies against the NF-L chain as a predictive biomarker of treatment response in multiple sclerosis. PMID: 24515731
21. a novel heterozygous missense mutation c.1166A>G (p.Y389C) in the gene encoding the light-chain neurofilament protein was identified in 4 patients resulting in hereditary motor and sensory neuropathy with pyramidal signs phenotype. PMID: 25583183
22. Data suggest that, while lacking a stable structure, NFL-TBS.40-63 peptide (a peptide derived from light neurofilament protein) preferentially binds on a specific single site located near C-terminal end of beta-tubulin. PMID: 26016807
23. Blood-derived NfL level is an easily accessible biomarker with prognostic value in ALS. PMID: 25934855
24. Data indicate that the N terminus of Pro-EMAP II binds to its C terminus, arginyl-tRNA synthetase, and the neurofilament light subunit. PMID: 25724651
25. an NEFL mutation in a family clinically manifesting congenital myopathy PMID: 25264603
26. NEFL overexpression also enhanced differentiation. PMID: 25312269
27. This is the first study to suggest blood NFL mRNA as a surrogate marker for early prediction of prediabetic peripheral neuropathy, while NSE mRNA levels may be of no diagnostic value in prediabetic patients. PMID: 24733614
28. An association was found with high CSF NEFL levels and severity of neurodegenerative diseases and survival. PMID: 25339208
29. expression of NEFL induces cancer cell apoptosis and inhibits invasion in these cell lines, suggesting that NEFL may play a role in cancer cell apoptosis and invasion. PMID: 23992471
30. CSF NFL indicates ongoing axonal injury in many neuroasymptomatic HIV patients PMID: 24523921
31. Our findings reveal an extended phenotype of Charcot Marie Tooth disease 2E caused by an identical missense mutation of the NEFL gene. PMID: 24887401
32. The expression of two miRNAs (miRNAs miR-b1336 and miR-b2403) whose effect is to stabilize NEFL mRNA, are down regulated in amyotrophic lateral sclerosis. PMID: 24454911
33. Neurofilament light chain protein NF-L has a dynamic role in the reactive and regenerative changes in axons following injury. PMID: 23802559
34. Within 7 days after cardiac arrest, serum NF-L is a valuable marker of long-term poor neurological outcome. PMID: 23287695
35. Increased neurofilament light chain blood levels are associated with neurodegenerative neurological diseases PMID: 24073237
36. NEFL is downregulated by hypermethylation in breast cancer. PMID: 24026393
37. CSF neurofilament light chain is elevated in frontotemporal degeneration and correlates with disease severity. PMID: 24242746
38. The biomarker pattern of neurofilament light in CSF distinguishes between progressive and static neurologic disorders. PMID: 23827424
39. A group of dysregulated miRNAs directly regulate the NFL mRNA 3'UTR in amyotrophic lateral sclerosis. PMID: 23705811
40. Anti-NFL antibodies could be surrogate biomarkers of axonal injury in early multiple sclerosis. PMID: 23870535
41. High CSF NFL levels were found in patients with amyotrophic lateral sclerosis ( PMID: 22680408
42. Depressed neurofilament expression associates with apolipoprotein E3/E4 genotype in maturing human fetal neurons exposed to HIV-1. PMID: 22302611
43. NEFL mRNA is ectopically expressed in breast malignancies and could be a potential prognostic factor for early-stage breast cancer patients PMID: 22319610
44. Identification of gaiting defects combined with previously observed muscle atrophy, reduced axon caliber, and decreased nerve conduction velocity suggests that hNF-L(E397K) mice recapitulate many clinical signs associated with Charcot-Marie-Tooth disease. PMID: 22288874
45. Cerebrospinal fluid biomarker NF-L in white matter lesions differentiates patients with subcortical vascular disease from those with Alzheimer's. PMID: 21860087
46. Subjects with APOE epsilon4 have higher CSF NFL levels than non-epsilon4 carriers, only when they do not carry a short poly-T variant of TOMM40, which is associated with later age of onset of AD. PMID: 21983493
47. Neurofilament light polypeptide (NEFL) was identified as a novel hypermethylated gene associated with resistance to cisplatin-based chemotherapy in cancer of head and neck. PMID: 22246235
48. Normal cerebrospinal fluid levels of NFL at 12 months post-operatively and beyond suggest the absence of any long-term neuronal damage caused by long-term deep brain stimulation treatment in Parkinson's disease. PMID: 21039366
49. NFL levels were not significantly increased in multiple sclerosis patients compared with controls and had no relationship to disability or progression PMID: 21197541
50. While RGNEF and NFL mRNA interact directly in vitro, interestingly they only appear to interact in amyotrophic lateral sclerosis lysates and not in controls PMID: 19488899
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相關疾?。?/div>
Charcot-Marie-Tooth disease 1F (CMT1F); Charcot-Marie-Tooth disease 2E (CMT2E)
亞細胞定位：

Cell projection, axon. Cytoplasm, cytoskeleton.
蛋白家族：

Intermediate filament family
數據庫鏈接：

HGNC: 7739

OMIM: 162280

KEGG: hsa:4747

UniGene: Hs.521461