Recombinant Human Neuroepithelial cell-transforming gene 1 protein (NET1)

中文名稱：

人NET1重組蛋白
品名簡稱：

Recombinant Human NET1 protein
貨號：

CSB-EP768775HU
說明書：

MSDS Datasheet
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

NET1
Uniprot No.：

Q7Z628
別名：

ARHG8_HUMAN; ARHGEF8; GLYT2 ; Guanine nucleotide regulatory protein oncogene; MGC114559 ; MGC127767 ; MGC53602 ; mNET1; Net1; NET1A; Neuroepithelial cell transforming gene 1; Neuroepithelial cell-transforming gene 1 protein; p65 Net1 proto oncogene; Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8; Rho guanine nucleotide exchange factor GEF 8
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Isoform 2
來源：

E.coli
分子量：

77.9kDa
表達區域：

1-542aa
氨基酸序列

MVAHDETGGLLPIKRTIRVLDVNNQSFREQEEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
基因功能參考文獻：
1. data suggest that NET-1 promotes HCC invasion, adhesion and growth by regulating BAX, caspase 3, caspase 8 and BCL2 expression. PMID: 30301500
2. Study found NET1 as a direct target for miR-22 to promote chemoresistance of bladder cancer cells. PMID: 29620229
3. We suggest that the oncogenic properties of CML cells are probably due to deregulated expression of NET1 as a result of altered expression of miR-22. PMID: 25041463
4. Net1 promotes the angiogenesis in cervical squamous cell carcinomas and siRNA targeting of Net1 can effectively reduce angiogenesis and inhibit tumor growth via VEGF signaling. PMID: 28511963
5. AMPK phosphorylation of the RHOA guanine nucleotide exchange factor NET1A inhibits extracellular matrix degradation, an early step in cell invasion PMID: 26456332
6. Net1 mRNA and protein levels in non-small cell lung cancer tissues were significantly elevated compared with those in their corresponding nontumor tissues. PMID: 26459749
7. Simultaneous silencing of NET-1 and survivin using one-chain-double-target siRNA thus provides an advantageous alternative in the development of therapeutics for skin squamous cell carcinoma . PMID: 26080853
8. These data indicate that Net1A acetylation regulates its subcellular localization to impact on RhoA activity and actin cytoskeletal organization. PMID: 25588829
9. NET1 was associated with comorbidity of oppositional defiant disorder and symptoms in Attention-deficit/hyperactivity disorder probands PMID: 24942521
10. Net1A plays a plays a previously unrecognized, Rho GTPase-independent role in controlling ATM activity and downstream signaling after DNA damage to impact cell survival. PMID: 25486363
11. High expression of neuroepithelial transforming gene 1 was associated with hepatocellular carcinoma. PMID: 25113254
12. The overexpression of NET-1 in tumor cells may be closely related to the malignant phenotype of skin squamous cell carcinoma including proliferation, invasion and tumor growth. PMID: 24196235
13. NET1 expression is elevated in OAC and its pre-malignant phenotype, Barrett's oesophagus. NET1 promotes OAC cell invasion and proliferation. PMID: 23945136
14. NET-1 and VEGF silencing play a key role in inhibiting hepatocellular cell proliferation, promoting apoptosis, and reducing angiogenesis. PMID: 23636606
15. Results identify Net1 as a novel regulator of mitosis and indicate that altered expression of Net1, as occurs in human cancers, may adversely affect genomic stability. PMID: 23864709
16. Data suggests that NET1 may be of prognostic significance in the clinical management of gastric adenocarcinoma PMID: 24375299
17. A combination of NET1 (rs2242447) and SNAP-25 (rs3746544) is a risk factor for ADHD. PMID: 23872233
18. A previously unrecognized role for the Net1A isoform in controlling FAK activation during planar cell movement. PMID: 23689132
19. Rac1 activation causes relocalization of Net1 isoforms outside the nucleus and stimulates Net1A catalytic activity. PMID: 23184663
20. Net -1 frameshifting on a noncanonical sequence in a herpes simplex virus drug-resistant mutant is stimulated by nonstop mRNA. PMID: 22927407
21. Net1 is a CARMA-interacting molecule and brings important information on the molecular mechanisms that control NF-kappaB transcriptional activity PMID: 22343628
22. A mechanism involving the nuclear GEFs Ect2 and Net1 for activating RhoB after genotoxic stress, thereby facilitating cell death after treatment with DNA damaging agents. PMID: 21373644
23. Existence of a functional Net1/RhoA signaling pathway within the nucleus of the cell and this implicates them in the DNA damage response. PMID: 21390328
24. NET1 plays an role in gastric cancer cell migration and invasion. The gene expression profile further elucidates the molecular mechanisms underpinning NET1-mediated aggressive GC cell behaviour. PMID: 21284875
25. RNAi targeting NET-1 gene effectively down-regulates the expression of NET-1 in A431 cells, leading to an inhibition of proliferation, migration and infiltration. PMID: 20078975
26. Smad3 regulates RhoA activation and cytoskeletal reorganization by controlling NET1 in TGF-beta1-induced ARPE-19 cells. PMID: 20547485
27. NET-1 protein is expressed in cytoplasm of HCC cells as irregular granules near Golgi apparatus, and may promote the uncontrolled proliferation and abnormal differentiation in HCC cells. PMID: 18478931
28. NET1 has roles in stimulating cytoskeletal reorganization and in transforming cells PMID: 15611121
29. PAK1 negatively regulates the activity of NET1 PMID: 15684429
30. The putative role for NET1 genes may provide important targets for intervention in Gastric adenocarcinoma, evidenced by their role in promoting invasion and proliferation, key phenotypic hallmarks of cancer cells. PMID: 16552434
31. These results suggest that the interaction between Net1 and Dlg1 may contribute to the mechanism of Net1-mediated transformation. PMID: 17938206
32. study concludes expression of NET-1 may relate to proliferation, metastasis & clinic stages of hepatocellular carcinoma (HCC); expression in HCC tissues may positively correlate to TMN stages; expression in HCC tissues was positively correlated with PCNA PMID: 18214716
33. data highlight the importance of Net1 in controlling RhoA and p38 MAPK mediated cell survival in cells exposed to DNA damaging agents PMID: 18509476
34. the importance of NET1 as a driver of tumour cell invasion PMID: 18827818
35. NET1 and integrin alpha6beta4 coexpression is independently associated with the development of distant metastasis in breast cancer. PMID: 19124484
36. Net1 requires interaction with PDZ domain proteins, such as Dlg1, to protect it from proteasome-mediated degradation and to maximally stimulate RhoA and that this interaction is regulated by cell-cell contact. PMID: 19586902
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亞細胞定位：

Cytoplasm. Nucleus.
組織特異性：

Widely expressed.
數據庫鏈接：

HGNC: 14592

OMIM: 606450

KEGG: hsa:10276

STRING: 9606.ENSP00000347134

UniGene: Hs.25155