Recombinant Human NACHT, LRR and PYD domains-containing protein 12 (NLRP12), partial

中文名稱：

人NLRP12重組蛋白
貨號：

CSB-YP015867HU
規格：
來源：

Yeast
其他：

中文名稱：

人NLRP12重組蛋白
貨號：

CSB-EP015867HU
規格：
來源：

E.coli
其他：

中文名稱：

人NLRP12重組蛋白
貨號：

CSB-EP015867HU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人NLRP12重組蛋白
貨號：

CSB-BP015867HU
規格：
來源：

Baculovirus
其他：

中文名稱：

人NLRP12重組蛋白
貨號：

CSB-MP015867HU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

NLRP12
Uniprot No.：

P59046
別名：

CLR19.3; FCAS2; Monarch 1; Monarch-1; NACHT, leucine rich repeat and PYD containing 12; NACHT, LRR and PYD containing protein 12; NACHT, LRR and PYD domains-containing protein 12; NAL12_HUMAN; NLR family, pyrin domain containing 12; NLRP12; Nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 12; OTTHUMP00000067227; PAN6; PYPAF7; PYRIN containing APAF1 like protein 7; PYRIN-containing APAF1-like protein 7; Regulated by nitric oxide; RNO; RNO2
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays an essential role as an potent mitigator of inflammation. Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways. Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway. In turn, promotes bacterial tolerance. Inhibits also the DDX58-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked DDX58 activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked DDX58 degradation. Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue.
基因功能參考文獻：
1. Patients with NLRP12 germline mutations and diagnosis of NLRP12-related autoinflammatory disease demonstrated highly variable clinical phenotypes. PMID: 29500522
2. report the crystal structure of NLRP12 PYD domain at 1.70 A fused with an maltose-binding protein (MBP) tag PMID: 29293680
3. all of the reported mutations were found to have occurred in a highly conserved region in the NACHT domain coding sequence in NLRP12 exon 3, suggesting that a screening strategy for Familial cold autoinflammatory syndrome should focus on this area of the gene PMID: 27314497
4. The novel findings reveal the critical role of NLRP12-IL-17A-CXCL1 axis in host defense by modulating neutrophil recruitment against Klebsiella pneumoniae. PMID: 26349659
5. This process involved the upregulation of NLRP12. PMID: 26329693
6. Variants of NLRP12 were associated with common variable immunodeficiency. PMID: 26122175
7. NLRP12/NLRP3-dependent activation of caspase-1 is likely to be a key event in mediating systemic production of IL-1beta and hypersensitivity to secondary bacterial infection during malaria. PMID: 24453977
8. The genetics, expression and roles of NLRP12 in inflammatory signaling, host defense, and carcinogenesis are reviewed. Review. PMID: 25249449
9. This study suggested that NLRP12 mutations might account for a small fraction of common variable immunodeficiency patients with severe auto-inflammatory complications. PMID: 25064839
10. We will focus on NLRP6 and NLRP12. PMID: 24338634
11. Letter: NLRP12 mutations associated with familial cold autoinflammatory syndrome 2 in Italian patients. PMID: 24064030
12. Even with a variable range of associated manifestations, the extreme sensitivity to cold represents the main clinical hallmark in an individual carrying the p.D294E mutation of the NLRP12 gene. PMID: 21360512
13. The authors report the first in vitro confirmed non-homotypic interaction between NLRP12 pyrin domain and the pro-apoptotic protein Fas-associated factor 1 (FAF-1), which links the innate immune system to apoptotic signaling. PMID: 21978668
14. NLRP12 missense mutation in periodic fever syndromes; study provides evidence of deleterious effect of an NLRP12 missense mutation; this newly identified molecular defect emphasizes the crucial role played by NLRP12 in autoinflammation PMID: 21538323
15. regulates activation of NF-kappa B and caspase-1-dependent cytokine processing PMID: 12019269
16. Monarch-1 enhances nonclassical and classical major histocompatibility complex class I expression at the level of the promoter, RNA, and protein expression. PMID: 12759408
17. Monarch-1 associates with IRAK-1 but not MyD88, resulting in the blockage of IRAK-1 hyperphosphorylation PMID: 16203735
18. Monarch-1 inhibits CD40-mediated activation of NF-kappaB via the non-canonical pathway in human monocytes. This inhibition stems from the ability of Monarch-1 to associate with and induce proteasome-mediated degradation of NF-kappaB inducing kinase PMID: 17237370
19. Heat shock protein 90 is a critical regulator of Monarch-1 anti-inflammatory activity PMID: 17947705
20. ATP binding by NLRP12 is critical for its inhibitory function. PMID: 18160710
21. Two mutations, nonsense and splice site, were identified in NALP12 in two families with periodic fever syndromes. PMID: 18230725
22. Relative copy numbers for the inflammasome mRNAs for ASC, caspase-1, NALP1, and Pypaf-7 were significantly lower in patients with septic shock compared with critically ill control subjects. PMID: 18263805
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相關疾病：

Familial cold autoinflammatory syndrome 2 (FCAS2)
亞細胞定位：

Cytoplasm.
蛋白家族：

NLRP family
組織特異性：

Detected only in peripheral blood leukocytes, predominantly in eosinophils and granulocytes, and at lower levels in monocytes.
數據庫鏈接：

HGNC: 22938

OMIM: 609648

KEGG: hsa:91662

STRING: 9606.ENSP00000319377

UniGene: Hs.631573