Recombinant Human Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1)

中文名稱：

人IGF2BP1重組蛋白
品名簡稱：

Recombinant Human IGF2BP1 protein
貨號：

CSB-EP878937HUb1
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

IGF2BP1
Uniprot No.：

Q9NZI8
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

69.5 kDa
表達區域：

1-577aa
氨基酸序列

MNKLYIGNLNESVTPADLEKVFAEHKISYSGQFLVKSGYAFVDCPDEHWAMKAIETFSGKVELQGKRLEIEHSVPKKQRSRKIQIRNIPPQLRWEVLDSLLAQYGTVENCEQVNTESETAVVNVTYSNREQTRQAIMKLNGHQLENHALKVSYIPDEQIAQGPENGRRGGFGSRGQPRQGSPVAAGAPAKQQQVDIPLRLLVPTQYVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAAEKAISVHSTPEGCSSACKMILEIMHKEAKDTKTADEVPLKILAHNNFVGRLIGKEGRNLKKVEQDTETKITISSLQDLTLYNPERTITVKGAIENCCRAEQEIMKKVREAYENDVAAMSLQSHLIPGLNLAAVGLFPASSSAVPPPPSSVTGAAPYSSFMQAPEQEMVQVFIPAQAVGAIIGKKGQHIKQLSRFASASIKIAPPETPDSKVRMVIITGPPEAQFKAQGRIYGKLKEENFFGPKEEVKLETHIRVPASAAGRVIGKGGKTVNELQNLTAAEVVVPRDQTPDENDQVIVKIIGHFYASQMAQRKIRDILAQVKQQHQKGQSNQAQARRK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Plays a direct role in the transport and translation of transcripts required for axonal regeneration in adult sensory neurons. Regulates localized beta-actin/ACTB mRNA translation, a crucial process for cell polarity, cell migration and neurite outgrowth. Co-transcriptionally associates with the ACTB mRNA in the nucleus. This binding involves a conserved 54-nucleotide element in the ACTB mRNA 3'-UTR, known as the 'zipcode'. The RNP thus formed is exported to the cytoplasm, binds to a motor protein and is transported along the cytoskeleton to the cell periphery. During transport, prevents ACTB mRNA from being translated into protein. When the RNP complex reaches its destination near the plasma membrane, IGF2BP1 is phosphorylated. This releases the mRNA, allowing ribosomal 40S and 60S subunits to assemble and initiate ACTB protein synthesis. Monomeric ACTB then assembles into the subcortical actin cytoskeleton. During neuronal development, key regulator of neurite outgrowth, growth cone guidance and neuronal cell migration, presumably through the spatiotemporal fine tuning of protein synthesis, such as that of ACTB. May regulate mRNA transport to activated synapses. Binds to and stabilizes ABCB1/MDR-1 mRNA. During interstinal wound repair, interacts with and stabilizes PTGS2 transcript. PTGS2 mRNA stabilization may be crucial for colonic mucosal wound healing. Binds to the 3'-UTR of IGF2 mRNA by a mechanism of cooperative and sequential dimerization and regulates IGF2 mRNA subcellular localization and translation. Binds to MYC mRNA, in the coding region instability determinant (CRD) of the open reading frame (ORF), hence prevents MYC cleavage by endonucleases and possibly microRNA targeting to MYC-CRD. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to the oncofetal H19 transcript and to the neuron-specific TAU mRNA and regulates their localizations. Binds to and stabilizes BTRC/FBW1A mRNA. Binds to the adenine-rich autoregulatory sequence (ARS) located in PABPC1 mRNA and represses its translation. PABPC1 mRNA-binding is stimulated by PABPC1 protein. Prevents BTRC/FBW1A mRNA degradation by disrupting microRNA-dependent interaction with AGO2. Promotes the directed movement of tumor-derived cells by fine-tuning intracellular signaling networks. Binds to MAPK4 3'-UTR and inhibits its translation. Interacts with PTEN transcript open reading frame (ORF) and prevents mRNA decay. This combined action on MAPK4 (down-regulation) and PTEN (up-regulation) antagonizes HSPB1 phosphorylation, consequently it prevents G-actin sequestration by phosphorylated HSPB1, allowing F-actin polymerization. Hence enhances the velocity of cell migration and stimulates directed cell migration by PTEN-modulated polarization. Interacts with Hepatitis C virus (HCV) 5'-UTR and 3'-UTR and specifically enhances translation at the HCV IRES, but not 5'-cap-dependent translation, possibly by recruiting eIF3. Interacts with HIV-1 GAG protein and blocks the formation of infectious HIV-1 particles. Reduces HIV-1 assembly by inhibiting viral RNA packaging, as well as assembly and processing of GAG protein on cellular membranes. During cellular stress, such as oxidative stress or heat shock, stabilizes target mRNAs that are recruited to stress granules, including CD44, IGF2, MAPK4, MYC, PTEN, RAPGEF2 and RPS6KA5 transcripts.
基因功能參考文獻：
1. lncRNA THOR is up-regulated in retinoblastoma, and its over-expression significantly enhances the malignant phenotype transformation of retinoblastoma cells by up-regulating c-myc and TGF2BP1 expression. PMID: 30119193
2. SOX12 can increase the expression of CDK4 and IGF2BP1, which confer malignant phenotypes to Hepatocellular Carcinoma. PMID: 28975985
3. report insulin-like growth factor-II binding protein 1 (IGF2BP1) as a novel interacting partner of p38 MAPK. PMID: 28497370
4. IGF2BP1 mediates posttranscriptional loss of BCL11A in cultured human adult erythroblasts PMID: 28652347
5. HCG11 exerted its effect on Hepatocellular carcinoma (HCC)via interaction with IGF2BP1, leading to activation of MAPK signaling, which eventually promoted the progression of HCC PMID: 28677801
6. miR-98-5p, downregulated in HCC, inhibits proliferation while inducing apoptosis in HCC LM3 cells, partly at least, through directly targeting IGF2BP1 PMID: 28244848
7. Findings suggest a MicroRNA-140-5p (miR-140-5p)-Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1) regulatory circuit for CC pathogenesis, and miR-140-5p may be a potential target for CC therapy. PMID: 27588393
8. Our studies provide insights into a molecular mechanism that the positive function of IMP1 to inhibit breast tumor growth and metastasis PMID: 26910917
9. The regions encoding 71 microRNAs (miRs) were deleted in at least 25% of tumor specimens. Five of these recurrently deleted miRs targeted the insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) gene product, and a correlating 100-fold upregulation of IGF2BP1 mRNA was seen in tumor specimens PMID: 28486549
10. Insight into molecular interactions between CRD-BP and MITF mRNA. PMID: 28182633
11. IGF2BP1 indirectly potentiates ETV6/RUNX1-RAC1-STAT3 signaling axis by sustaining appropriate ETV6/RUNX1 and STAT3 transcript levels in REH cells. PMID: 27239736
12. The findings indicate that the tumor-suppressive roles of the let-7 family are antagonized by a potent and self-promoting triangle consisting of IGF2BP1 (potentially all IGF2BPs), LIN28B and HMGA2. PMID: 26917013
13. The results from this study demonstrate the potential importance of the two-stem-loop motif as a target region for the inhibition of the CRD-BP-GLI1 RNA interaction and Hedgehog signaling pathway. PMID: 27036131
14. these findings suggest that miR-150 functions as a tumor suppressor in Osteosarcoma (OS) partially by targeting IGF2BP1 PMID: 26561465
15. ETV6/RUNX1 transcript is a target of RNA-binding protein IGF2BP1 in t(12;21)(p13;q22)-positive acute lymphoblastic leukemia. PMID: 26852652
16. Data show that ONECUT2, IGF2BP1, and ANXA2 proteins were confirmed to be microRNA-9 (miR-9) targets and aberrantly upregulated in hepatocellular carcinoma (HCC). PMID: 26547929
17. The tumor-suppressive role of stromal IMP1 and its ability to modulate protumorigenic factors suggest that IMP1 status is important for the initiation and growth of epithelial tumors PMID: 26194191
18. IGF2BP1 as a direct and functional target of miR-196b. PMID: 25889892
19. Data show that insulin-like growth factor-2-mRNA-binding proteins IGF2BP1, IGF2BP2, and IGF2BP3 are direct targets of microRNA-1275 (miR-1275). PMID: 26160756
20. High CRD-BP expression is associated with Breast Tumor. PMID: 25861986
21. Data demonstrate that IGF2BP1 is a potential oncogene and an independent negative prognostic factor in neuroblastoma. PMID: 25753434
22. Results identify IGF2BP1 as a critical translational regulator of cIAP1-mediated apoptotic resistance in rhabdomyosarcoma. PMID: 24704827
23. miR-873 negatively affected the carcinogenesis and metastasis of GBM by down-regulating the expression of IGF2BP1, which stabilizes the mRNA transcripts of its target genes PMID: 25670861
24. These results indicate that IGF2BP1 and TET1/2 contribute to the stemness of MSCs, at least regarding their proliferative potential. PMID: 24915579
25. MicroRNA-340-mediated degradation of MITF mRNA is inhibited by CRD-BP. PMID: 25414259
26. Two KH domains of CRD-BP are required for efficient binding to oncogenic mRNAs and for granule formation in zebrafish embryos. PMID: 25389298
27. A 14;17 translocation resulting in an IGH-IGF2BP1 fusion is associated with B acute lymphoblastic leukemia. PMID: 25195122
28. CRD-BP is overexpressed in basal cell carcinoma and its expression positively correlates with the activation of both Wnt and Hh signaling pathways PMID: 24468749
29. The RNA-binding protein IGF2BP1 is an important protumorigenic factor in liver carcinogenesis. PMID: 24395596
30. Data suggest GHET1 (gastric carcinoma high expressed transcript 1) is prognostic factor for gastric carcinoma; GHET1 promotes c-Myc (proto-oncogene proteins c-Myc) mRNA stability/expression, binds IGF2BP1, and promotes gastric carcinoma growth. PMID: 24397586
31. IGF2BP1 acts as an adaptor protein that recruits the CCR4-NOT complex and thereby initiates the degradation of the lncRNA HULC. PMID: 23728852
32. Shows role for CRD-BP in the regulation of melanoma cell invasion and highlights the importance of the hypoxic microenvironment in determining cell fate. PMID: 23038779
33. our study suggests that IMP1might play an important role in the progression of choriocarcinoma through the regulation of cell migration and invasion. PMID: 23911878
34. in both T47D and MDA231 human breast carcinoma cells IMP1/ZBP1 functions to suppress cell invasion PMID: 22266909
35. IGF2BP1 is a potent oncogenic factor that regulates the adhesion, migration and invasiveness of tumor cells by modulating intracellular signaling. PMID: 22983196
36. The defective splicing caused by the ISCU intron mutation in patients with myopathy with lactic acidosis is repressed by PTBP1 but can be derepressed by IGF2BP1. PMID: 22125086
37. Data show that the let-7 (let-7d and let-7g) target IMP-1 stabilizes the mRNA of MDR1. PMID: 21618519
38. IGF2BP1 promotes the velocity and directionality of tumor-derived cell migration by determining the cytoplasmic fate of two novel target mRNAs: MAPK4 and PTEN PMID: 22279049
39. sh-RNA knockdown of CRD-BP enhances the effect of dacarbazine, temozolomide, vinblastine, & etoposide on both primary and metastatic melanoma cell lines. PMID: 21981993
40. Studies identify a novel proapoptotic gene target, CYFIP2, which is downregulated by IMP-1, and mediates the regulation of cell survival and K-Ras expression in colon cancer cells. PMID: 21252116
41. IMP1 plays a role in regulating the packaging of MLV genomic RNA and can be used for improving production of retroviral vectors PMID: 21209918
42. IGF2BP1 genotype, haplotype and genetic model studies in metabolic syndrome traits and diabetes PMID: 20627640
43. IMP1 localization is associated with motility and the major functions of IMP1 are carried out by the phylogenetically conserved KH domains PMID: 11973350
44. Aberrant CRD-BP expression may interfere with c-myc regulation. Copy number gains at 8q24 (c-myc), were seen in 48.3% of tumors, gains at 17q21 (CRD-BP) in 18.3%,& CRD-BP was seen in 58.5%, implying mechanisms of activation other than gene amplification. PMID: 12532419
45. IMP1 translocates to the nucleus and contains nuclear export signals within the RNA-binding KH2 and KH4 domains. PMID: 12921532
46. IMP-1 may have a role in progression of ovarian cancer PMID: 14767552
47. Intestinal epithelial cells continue to express IMP1 postnatally, and Imp1(-/-) mice exhibited impaired development of the intestine, with small and misshapen villi and twisted colon crypts. PMID: 15121863
48. highest frequency of CRD-BP positive tumors was observed in meningiomas PMID: 15159028
49. intimate association of fragile X mental retardation protein and IMP1 suggests a link between mRNA transport and translational repression in mammalian cells PMID: 15282548
50. A cooperative mechanism for the binding of IMP-1 to RNA. PMID: 15314207
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亞細胞定位：

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Cell projection, lamellipodium. Cell projection, dendrite. Cell projection, dendritic spine. Cell projection, growth cone. Cell projection, filopodium. Cell projection, axon.
蛋白家族：

RRM IMP/VICKZ family
組織特異性：

Mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus (at protein level). Also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta (at protein level). Expre
數據庫鏈接：

HGNC: 28866

OMIM: 608288

KEGG: hsa:10642

STRING: 9606.ENSP00000290341

UniGene: Hs.144936