Recombinant Human Glutamate receptor ionotropic, NMDA 1 (GRIN1), partial

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中文名稱：

人GRIN1重組蛋白
品名簡稱：

Recombinant Human GRIN1 protein, partial
貨號：

CSB-EP009911HU
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

GRIN1
Uniprot No.：

Q05586
別名：

GluN1; Glutamate [NMDA] receptor subunit zeta-1; Glutamate receptor ionotropic N methyl D aspartate 1; Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1; glutamate receptor ionotropic, NMDA 1; Grin1; MRD8; N methyl D aspartate receptor; N methyl D aspartate receptor channel subunit zeta 1; N methyl D aspartate receptor subunit NR1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1; NMDA 1; NMDA R1; NMDA receptor 1; NMDA1; NMDAR; NMDZ1_HUMAN; NR1
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

64.6 kDa
表達區域：

19-559aa
氨基酸序列

RAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYSWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEAKELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGILGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTLSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The DNA fragment encoding the 19-559aa of the human GRIN1 protein was fused with N-terminal 6xHis tag gene and then was inserted into the expression vector, which was subsequently transformed into the E.coli for expression. The resulting product was further purified to obtain the recombinant human GRIN1 protein. The purity of this recombinant GRIN1 protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This recombinant GRIN1 protein showed a band on the gel with a molecular weight of approximately 60 kDa.

As a novel protein of neuromuscular junction and brain, GRIN1 plays essential roles including learning, memory, and synaptic plasticity. For Alzheimer's disease (AD), GRIN1 recently has been found to play a role in regulation of AD pathway. For Parkinson's disease (PD), gene co-expression network analysis identifies that hub gene GRIN1 is related to Parkinson's disease. GRIN1 that interacts with SLC18A2 is involved in the interaction of neuroactive ligand receptors. Others suggested that GRIN1 can be associated with PD in early or later stages.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition.
基因功能參考文獻：
1. Glycans potentiate the effect of GluN1 and GluN2B receptors. PMID: 28378791
2. In this study, we report a success of the WES approach to the identification of a genetic cause of a challenging case, undiagnosed on clinical grounds. We identified the causative missense mutation (p.Met727Val) in exon 16 of the GRIN1 gene. As the p.Met727Val mutation shown by WES is in the same GluN1 domain, we infer that the pathogenic variant impact on NMDAR is likely similar to that induced by p.Glu662Lys mutation. PMID: 29194067
3. one base difference in the GRIN1M promoter sequence (G --> C) results in the inability of the sequence to form a parallel G-quadruplex. PMID: 28702665
4. Data suggest GRINL1A (GCOM1)-NMDA receptor-internexin-alpha (INA) interaction pathway may be relevant to neuroprotection. PMID: 29339073
5. These results indicate these individuals may have suffered neurodevelopmental deficits as a result of the decreased presence of GluN1-G620R/GluN2B complexes on the neuronal surface during embryonic brain development and reduced current responses of GluN1-G620R-containing NMDARs after birth. PMID: 28228639
6. Mice with GRIN1 disrupted in the intralaminar thalamic nuclei exhibited various schizophrenia-like phenotypes, including deficits in working memory, long-term spatial memory, and attention, as well as impulsivity, impaired prepulse inhibition, hyperlocomotion and hyperarousal. PMID: 28244984
7. 2-methoxyestradiol impacts on glycine/serine-mediated metabolic reprogramming in osteosarcoma cells by its interaction with GRIN1/GluN2A receptors. PMID: 28262924
8. tPA is a ligand of the N-terminal domain of the obligatory GluN1 subunit of NMDAR acting as a modulator of their dynamic distribution at the neuronal surface and subsequent signaling. PMID: 27831563
9. Two novel Grin1 mutations were identified in 2 cases of severe early infantile encephalopathy. Se688Tyr mutation results in disruption of NMDA ligand binding and the p.Gly827Arg mutation results in disrupted gating of the ion channel. PMID: 28389307
10. A homozygous missense variant of GRIN1 was identified in two consanguineous sibs affected with severe intellectual disability and autistic features. PMID: 28051072
11. NMDA receptor-dependent signaling is involved in melanosome transfer, which is associated with calcium influx, cytoskeleton protein redistribution, dendrites and filopodia formation PMID: 27596138
12. Findings show that N-methyl-d-aspartic acid receptor subunit GluN1 is expressed on oligodendrocytes and myelin in humans. PMID: 27443784
13. De novo GRIN1 mutations are associated with severe intellectual disability with cortical visual impairment as well as oculomotor and movement disorders being discriminating phenotypic features. Loss of NMDA receptor function appears to be the underlying disease mechanism. The identification of both heterozygous and homozygous mutations blurs the borders of dominant and recessive inheritance of GRIN1-associated disorders. PMID: 27164704
14. The differences in cortical NMDAR expression and post-synaptic density protein 95 are present in psychiatric disorders and suicide completion and may contribute to different responses to ketamine. PMID: 26013316
15. GRIN1 (rs4880213) was significantly associated with depression and disruptive behavior in adolescents. PMID: 26819771
16. Knockdown of PKD1 did not affect NMDAR internalization but prevented the phosphorylation and inhibition of remaining surface NMDARs and NMDAR-mediated synaptic functions. PMID: 26584860
17. Study found GluN receptor subunit-specific changes in mixed subcortical ischemic vascular dementia(SIVD)/Alzheimer's disease(AD) (decreased GluN1) and SIVD (increased GluN2A and 2B), likely reflecting interaction of ischemic neurovascular and AD processes PMID: 25261450
18. results suggest that NMDA-R autoantibodies are unlikely to account for a large proportion of treatment-refractory psychosis. PMID: 25431428
19. The results of this study suggested that GRIN1 mutations cause encephalopathy resulting in seizures and movement disorders. PMID: 25864721
20. Genome-wide significant marker, SNP rs524991, and an association of seropositivity with influenza autoantibodies status, we provide genetic and environmental risk factors of NMDAR-autoantibodies formation PMID: 23999527
21. Epigenetic changes in GRIN1, in combination with experiences of maltreatment, may confer risk for depression in children. PMID: 24655651
22. Reduction in NR1 and NR2C in the DLPFC of people with schizophrenia may lead to altered NMDAR stoichiometry and provides compelling evidence for an endogenous NMDAR deficit in schizophrenia. PMID: 23070074
23. Isolated GluN1/GluN3A receptors integrated into lipid bilayers responded to addition of either glycine or d-serine, but not glutamate, with a approximately 1 nm reduction in height of the extracellular domain PMID: 25017909
24. Results show that the expression and distribution of NMDA receptors subunits GluN1, GluN2A and GluN2B - together with that of postsynaptic protein PSD-95 - are modified in Alzheimer's disease compared to normal aging PMID: 24156266
25. B7T inhibition of NMDA current mediated by NR1/NR2B receptor. PMID: 23271275
26. The rs1126442, GRIN1 polymorphism contributes to the genetic vulnerability to psychosis in METH-dependent subjects in the Thai population. PMID: 23880023
27. Association of multiple sclerosis disease severity and allelic variants of the NR1 and NR2B glutamate receptor genes. PMID: 23840674
28. GluN1 binds specifically to the sigma-1 receptor within intact cells. PMID: 24227730
29. Antibodies that bind recombinant GluN1-S2 peptides (but not the intact GluN1 protein) develop transiently in patients after stroke in proportion to infarct size, suggesting that these antibodies are raised secondarily to neuronal damage. PMID: 23723305
30. Transgenic NR1 receptors on neuradrenergic neurons regulate development of opiate dependence and psychomotor sensitization. PMID: 22040728
31. After 7 days of chronic alcohol exposure, there are significant increases in mRNA expression of GRIN1 in cultured neurons derived from alcoholic subjects, but not in cultures from nonalcoholics. PMID: 22486492
32. Adult NR1-deficient transgenic mice show multiple abnormal behaviors including reduced social interactions, locomotor hyperactivity, self-injury, deficits in prepulse inhibition and sensory hypersensitivity, among others. PMID: 22726567
33. GRIN1 and GRIN2D appear instrumental to normal brain development and function in this study of rare and/or de novo mutation in neurodevelopmental disorders. PMID: 22833210
34. The multifunctional cytokine-like molecule HMGB1 released by activated, stressed, and damaged or necrotic cells can facilitate NMDAR-mediated cell responses. PMID: 22952988
35. A critical role of the single glutamine residue within the GluN1 M4 domain regulates surface delivery of functional NMDA receptors. PMID: 22937865
36. key amino acid residues within both NR1 and NR2B M3 domains contribute to the regulation of the surface expression of unassembled NR1 and NR2 subunits PMID: 22711533
37. The unique co-existence of SP and phospho-NMDAR1 in tendinopathy presumably reflects a tissue proliferative and nociceptive role. PMID: 22354721
38. GluN1(hypo) transgenic mice exhibit impairments on all tests of cognition that are employed, as well as reduced engagement in naturalistic behaviors, including nesting and burrowing. PMID: 22300668
39. The NR1 subunit of NMDA receptors is involved in amygdala hyperexcitability in some patients who have temporal lobe epilepsy. PMID: 20848605
40. G Protein-regulated inducer of neurite outgrowth (GRIN) modulates Sprouty protein repression of mitogen-activated protein kinase (MAPK) activation by growth factor stimulation PMID: 22383529
41. Transgenic mice with dopaminergic neuron-specific NMDAR1 deletion are impaired in a variety of habit-learning tasks, while normal in some other dopamine-modulated functions such as locomotor activities. PMID: 22196339
42. Homozygotes for the T allele in the rs4880213 GRIN1 SNP had reduced intracortical inhibition, as expected for enhanced glutamatergic excitation in these subjects. PMID: 21753020
43. NMDAR1 subunit expressed by primary afferent nerves of floxed mice plays an important role in the development of sensitized pain states. PMID: 20974228
44. Expression of NMDA receptors in lymphocytes is regulated by central nervous system, which controls the inflammation process. PMID: 20414717
45. The results of this study suggested that haplotypes of GRIN1 may influence responsiveness to ACTH. PMID: 20722663
46. Sp4 hypomorphic mice could therefore serve as a genetic model to investigate impaired NMDA functions resulting from loss-of-function mutations of human SP4 gene in schizophrenia and/or other psychiatric disorders. PMID: 20634195
47. Both tissue-type PA (tPA) and urokinase-type PA (uPA) bind to NMDA-R1 and reverse this effect, thereby enhancing acetylcholine-induced tracheal contractility. PMID: 20097831
48. Functional NMDA receptors are expressed by breast cancer and are important agents for maintaining cell growth and viability. PMID: 19784770
49. Polymorphisms in the GRIN1 and GRIN2B genes may serve as potential biomarkers for a reduced risk of PD among the Chinese population in Taiwan. PMID: 20438806
50. The neuronal coexistence of glutamate and NMDAR1, observed in painful tendinosis but not in controls, suggests a regulatory role in intensified pain signalling. PMID: 19422642
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相關疾?。?/div>
Neurodevelopmental disorder with or without hyperkinetic movements and seizures, autosomal dominant (NDHMSD)
亞細胞定位：

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, postsynaptic density.
蛋白家族：

Glutamate-gated ion channel (TC 1.A.10.1) family, NR1/GRIN1 subfamily
數據庫鏈接：

HGNC: 4584

OMIM: 138249

KEGG: hsa:2902

STRING: 9606.ENSP00000360608

UniGene: Hs.558334