Recombinant Human G2/mitotic-specific cyclin-B1 (CCNB1)

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品名簡稱：

Recombinant Human CCNB1 protein
貨號：

CSB-EP004806HUa0
說明書：

MSDS Datasheet
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

CCNB1
Uniprot No.：

P14635
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

52.4 kDa
表達區域：

1-433aa
氨基酸序列

MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVPKKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Essential for the control of the cell cycle at the G2/M (mitosis) transition.
基因功能參考文獻：
1. FOXM1 promotes proliferation in human hepatocellular carcinoma cells by transcriptional activation of CCNB1. PMID: 29705704
2. SNAP23 suppressed progression of cervical cancer and induced cell cycle G2/M arrest via upregulating p21(cip1) and downregulating CyclinB1 PMID: 29908998
3. Defective cyclin B1 induction by T-DM1 mediates acquired resistance in HER2-positive breast cancer cells. PMID: 28821558
4. Up-regulation of CCNB1 could be an indicator for invasiveness of pituitary adenomas.Up-regulation of CCNB1 could be an indicator for invasiveness of pituitary adenomas. PMID: 28601573
5. We identified the known APC/C regulator Cdh1 and the F-box protein Fbxl15 as specific modulators of N-cyclin B1-luciferase steady-state levels and turnover. Collectively, our studies suggest that analyzing the steady-state levels of luciferase fusion proteins in parallel facilitates identification of specific regulators of protein turnover. PMID: 28296622
6. Overexpression of cyclin B1 is correlated with poor survival in most solid tumors, which suggests that the expression status of cyclin B1 is a significant prognostic parameter in solid tumors. [review] PMID: 27903976
7. CDK9, in addition to CDK1, has roles in mediating the growth inhibitory effect of dinaciclib on cyclin B1 in triple negative breast cancer PMID: 27486754
8. Data show that Islet-1 (ISL1) activated the expression of cyclin B1 (CCNB1), cyclin B2 (CCNB2) and c-myc (c-MYC) genes by binding to the conserved binding sites on their promoters or enhancers. PMID: 27183908
9. XIAP is stable during mitotic arrest, but its function is controlled through phosphorylation by the mitotic kinase CDK1-cyclin-B1 at S40. PMID: 27927753
10. ZIC5 is highly upregulated in non-small cell lung cancer tumor tissues and may act as an oncogene by influencing CCNB1 and CDK1 complex expression PMID: 27663664
11. Knockdown of DRG2 elicited down-regulation of the major mitotic promoting factor, the cyclin B1/Cdk1 complex. PMID: 27669826
12. Mitochondrial Ribosomal Protein L10 regulates cyclin B1/Cdk1 (cyclin-dependent kinase 1) activity and mitochondrial protein synthesis in mammalian cells PMID: 27726420
13. Changes in expression and localization of cyclin B1 may constitute a part of the mechanism responsible for resistance of HL-60 cells to etoposide. PMID: 27297620
14. Data suggest that long non-coding RNA ZFAS1 may function as oncogene via destabilization of tumor suppressor protein p53 (p53) and through cyclin-dependent kinase 1 (CDK1)/cyclin B1 complex leading to cell cycle progression and inhibition of apoptosis. PMID: 26506418
15. exposing renal carcinoma cells to amygdalin inhibited cell cycle progression and tumor cell growth by impairing cdk1 and cyclin B expression PMID: 26709398
16. Sodium butyrate accelerates 3' UTR-dependent cyclin B1 decay by enhancing the binding of tristetraprolin to the 3' untranslated region of cyclin B1. PMID: 26555753
17. CDK1-Cyclin B1 activates RNMT, coordinating mRNA cap methylation with G1 phase transcription. PMID: 26942677
18. Our results demonstrate for the first time that the SYSADOA diacerein decreased the viability of human chondrosarcoma cells and induces G2/M cell cycle arrest by CDK1/cyclin B1 down-regulation. PMID: 26555773
19. CCNB1 is target of miRNA-410 since its overexpression reduces CCNB1 at protein and mRNA levels. PMID: 26125663
20. Germ cell tumors consistently overexpressed cyclin B1 independently of their responsiveness to chemotherapy or the presence of p53 mutations. Cyclin B1 was overexpressed by GCT cell lines carrying functional p53. PMID: 25982682
21. The authors postulate that the mechanism of cytomegalovirus pUL97-human cyclin B1 interaction is determined by an active pUL97 kinase domain. PMID: 26270673
22. Stereospecific phosphorylation of only the Ser-trans-Pro peptide by Cdk1-cyclin B1 PMID: 25603287
23. Pharmacological inhibition or siRNA-mediated knockdown of cdk1/CCNB1 induced proliferation arrest independent of MYCN status in neuroblastoma cells. PMID: 26029996
24. Expression of CDK1 Tyr15, pCDK1 Thr161, Cyclin B1 (total) and pCyclin B1 Ser126 in vulvar squamous cell carcinoma and their relations with clinicopatological features and prognosis. PMID: 25849598
25. BUB1B expression was highly correlated to CDC20 and CCNB1 expression in multiple myeloma cells, leading to increased cell proliferation. PMID: 25698537
26. Proximity ligation assay demonstrate proximity between S100A4 and cyclin B1 in vitro, while confocal microscopy showed S100A4 to localize to areas corresponding to centrosomes in mitotic cells prior to chromosome segregation. PMID: 26349943
27. Cyclin B1 could suppress the invasion and metastasis of colorectal cancer cells through regulating E-cadherin expression, which enables the development of potential intervention strategies for colorectal cancer. PMID: 25962181
28. CCNB1 is a biomarker for the prognosis of ER+ breast cancer and monitoring of hormone therapy efficacy PMID: 25044212
29. that CCNB1 contained many CD4 T cell epitopes, which are differentially recognized by pre-existing naive and memory CD4 T cells. PMID: 26136431
30. CCNB1 activation is associated with recurrence in non-muscle-invasive bladder cancer. PMID: 24714775
31. PKCa and Cyclin B1 are linked in a DAG-dependent mechanism that regulates cell cycle progression PMID: 25362646
32. Complete removal of cyclin B1 is essential to prevent the return of the spindle checkpoint following sister chromatid disjunction. PMID: 25483188
33. Data show that inappropriate overexpression of cyclin B1 causes non-specific cell death. PMID: 25415322
34. Cyclin B1 marks the restriction point for permanent cell cycle exit in G2 phase. PMID: 25486360
35. CCNB1 is activated by Chk1, exerts its oncogenic role in colorectal cancer cells, and may play a key role in the development of a novel therapeutic approach against colorectal cancer. PMID: 24971465
36. Stable association of Cdk1-cyclin B1 with phosphorylated separase counteracts this tendency and stabilizes separase in an inhibited yet activatable state PMID: 25659430
37. the results were indicative that cyclin B may hold independent prognostic significance, but further studies are required to assess this. PMID: 25315186
38. that MAD2B may play an important role in high glucose-mediated podocyte injury of diabetic nephropathy via modulation of Cdh1, cyclin B1, and Skp2 expression PMID: 25651564
39. Expression of cycle marker cyclin B1 differs between benign and malignant papillary breast lesions. PMID: 25501285
40. Phosphorylated Akt plays an important role in regulating the expression level of cyclin B1 by interacting with AR and increasing the transcriptional activity of AR. PMID: 24574517
41. Cyclin B1 expression was studied immunohistochemically in specimens from 241 patients with pancreatic cancer and was correlated with clinicopathological features and patient survival. PMID: 25106528
42. Cyclin B1 overexpression is associated with medullary thyroid carcinoma. PMID: 24488334
43. study indicates that genetic polymorphisms of CCNB1 and CDK1 are related to BC susceptibility, progression, and survival in Chinese Han women. PMID: 24386390
44. Parvovirus-induced depletion of cyclin B1 prevents mitotic entry of infected cells. PMID: 24415942
45. our data suggest that the non-canonical Hh pathway mediated through ptch1 and cyclin B1 is involved in the pathogenesis of NBCCS-associated KCOTs. PMID: 24840883
46. Allele-dependent transcriptional regulation of CCNB1 associated with the SNPs rs350099, rs350104, and rs164390 affects ISR risk through differential recruitment of NF-Y, AP-1, and SP1. PMID: 24395923
47. Cyclin B1 and cyclin B2 are interchangeable for ability to promote G2 and M transition in HeLa cells. PMID: 24324638
48. Our results indicated that CDB(cyclin B destruction box )-fused fluorescent protein can be used to examine the slight gene regulations in the reporter gene system PMID: 24416725
49. The cyclin B 3'UTR was not sufficient to enhance cyclin B synthesis. PMID: 24058555
50. Cyclin B1/Cdk1-mediated phosphorylation of mitochondrial substrates allows cells to sense and respond to increased energy demand for G2/M transition and, subsequently, to upregulate mitochondrial respiration for successful cell-cycle progression. PMID: 24746669
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亞細胞定位：

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
蛋白家族：

Cyclin family, Cyclin AB subfamily
數據庫鏈接：

HGNC: 1579

OMIM: 123836

KEGG: hsa:891

STRING: 9606.ENSP00000256442

UniGene: Hs.23960