Recombinant Human Friend leukemia integration 1 transcription factor (FLI1)

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中文名稱：

人FLI1重組蛋白
貨號：

CSB-EP008714HU
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

FLI1
Uniprot No.：

Q01543
別名：

(Proto-oncogene Fli-1)(Transcription factor ERGB)
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

57.0 kDa
表達(dá)區(qū)域：

1-452aa
氨基酸序列

MDGTIKEALSVVSDDQSLFDSAYGAAAHLPKADMTASGSPDYGQPHKINPLPPQQEWINQPVRVNVKREYDHMNGSRESPVDCSVSKCSKLVGGGESNPMNYNSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
基因功能參考文獻(xiàn)：
1. These results indicate that Fli1 deficiency promotes migration, proliferation and cell survival, while abating tube formation of endothelial cells, suggesting that Fli1 deficiency is potentially attributable to the development of both proliferative obliterative vasculopathy (occlusion of arterioles and small arteries) and destructive vasculopathy (loss of small vessels) characteristic of SSc vasculopathy. PMID: 28370536
2. Fli1 functioned as an oncogene in HCC carcinogenesis and it exerted its promoting metastatic effect primarily by modulating the matrix metalloproteinase (MMP)2 signaling pathway. PMID: 29138848
3. Decreased serum LIF levels may be associated with vasculopathy in systemic sclerosis (SSc) and that Fli1 deficiency may contribute to the inhibition of LIF-dependent biological effects on SSc endothelial cells by suppressing the expression of LIF, LIF receptor, and gp130. PMID: 29038846
4. The mouse (Fli1) and human Fli1 genes are similarly regulated by Ets factors in T cells. PMID: 19829305
5. In contrast, expression of Spi1/PU.1 in a Fli1 producing erythroleukemia cell line in which fli1 is activated, resulted in increased proliferation through activation of growth promoting proteins MAPK, AKT, cMYC and JAK2 PMID: 28586009
6. CXCL6 expression is upregulated by Fli1 deficiency in fibroblasts and endothelial cells, potentially contributing to the development of fibrosis and vasculopathy in the skin, lung, and heart of systemic sclerosis. PMID: 28507181
7. This is the first report presenting CD13 and FLI1 as important mediators of resistance to BRAF inhibition with potential as drug targets in BRAF inhibitors refractory melanoma. PMID: 29048432
8. This study uncovers FLI1 as an important driving factor that promotes tumor growth in SCLC through the miR-17-92 pathway. FLI1 may serve as an attractive target for therapeutic intervention of SCLC. PMID: 28410216
9. identification of two novel FLI1 variants (c.1010G>A and c.1033A>G) responsible for macrothrombocytopenia; study provides new insights into the phenotype, pathophysiology and diagnosis of FLI1 variant-associated thrombocytopenia PMID: 28255014
10. miR-145 acts as a tumor suppressor by directly reducing expression of FLI-1, and the miR-145/FLI-1 pathway is important for tumor progression in osteosarcoma PMID: 27304058
11. this paper provides a mechanistic insight into the regulation of angiogenesis by TLRs and confirm a central role of Fli1 in regulating vascular homeostasis. PMID: 29141862
12. In American cutaneous leishmaniasis caused by Leishmania braziliensis infection, MMP1 is regulated by factors other than FLI1, and the influence of IL-6 on MMP1 was independent of its effect on FLI1. PMID: 28119029
13. epithelial Fli1 deficiency might be involved in the systemic autoimmunity and selective organ fibrosis in systemic sclerosis (SSc). PMID: 28232470
14. study shows that FLI-1 is expressed variably in different subtypes of NSCLC, and its expression is related to clinicopathologic parameters and poorer prognosis PMID: 26317314
15. FLI1 and MMP9 position differently in prostate cancer than in normal tissue and prostate hyperplasia, whereas MMP2 is repositioned in both prostate cancer and hyperplasia. PMID: 26564800
16. FLI1 promoter hypermethylation is associated with Colorectal Cancer. PMID: 25472652
17. Concurrent exogenous expression of three transcription factors, GATA1, FLI1 and TAL1, enables large-scale production of megakaryocytes from human pluripotent stem cells. PMID: 27052461
18. This study for the first time identifies FLI1 as a clinically and functionally important target gene of metastasis, providing a rationale for developing FLI1 inhibitors in the treatment of breast cancer. PMID: 26156017
19. SLFN11 has a role as a transcriptional target of EWS-FLI1 and is a determinant of drug response in Ewing sarcoma PMID: 25779942
20. Fli1 is epigenetically suppressed and is a potential predisposing factor in the pathogenesis of systemic sclerosis. (Review) PMID: 26055516
21. erythrocyte lineage enforces exclusivity through upregulation of EKLF and its lineage-specific cytokine receptor (EpoR) while inhibiting both FLI-1 and the receptor TpoR (also known as MPL) for the opposing megakaryocyte lineage PMID: 26159733
22. Mutation in FLI1 is associated with Paris-Trousseau thrombocytopenia. PMID: 26316623
23. Fli-1 expression gradually increases in parallel with disease progression. PMID: 26305602
24. Simultaneous downregulation of KLF5 and Fli1 is a key feature underlying systemic sclerosis. PMID: 25504335
25. endothelial CCN1 downregulation at least partially due to Fli1 deficiency may contribute to the development of digital ulcers in systemic sclerosis patients PMID: 25421497
26. bosentan...increased DNA binding of Fli1 and the suppression of type I collagen expression in systemic sclerosis fibroblasts PMID: 24708674
27. The similarity among the phenotypes of EWS/FLI1- and EWS siRNA-transfected HeLa cells points to the inhibition of EWS as the key mechanism for the induction of midzone defects. PMID: 25483190
28. FLI1 levels are reduced in human breast cancer samples and cell lines.FLI1 expression is correlated with breast cancer cellular growth, migration, and invasion and altered gene expression. PMID: 25379017
29. p16 and possibly Fli-1 may have utility in the assignment of growth phase for cutaneous melanomas. PMID: 25062258
30. Results show that overexpression is intimately related to malignant phenotypes and poor clinical outcome of epithelial ovarian cancer. PMID: 24923303
31. Fli1 bound to the CXCL5 promoter and its gene silencing significantly suppressed the CXCL5 mRNA expression in human dermal microvascular endothelial cells PMID: 24292093
32. Report FLI1 expression in epithelioid sarcomas. PMID: 24072183
33. Fli1 and CTGF are important mediators of the fibrogenic actions of adenosine PMID: 23663495
34. these results indicate that under quiescent conditions Fli1 recruits HDAC1/p300 to the COL1A2 promoter and suppresses the expression of the COL1A2 gene by chromatin remodeling through histone deacetylation. PMID: 24058639
35. these results fill a gap in the literature: the confirmation that MCC is devoid of the EWS/FLI-1 rearrangement. PMID: 23165331
36. Overexpression of the ETS-related transcription factor ETV1 can initiate neoplastic transformation of the prostate. PMID: 23774214
37. alterations in FLI1 and RUNX1 may be common in patients with platelet dense granule secretion defects and mild thrombocytopenia. PMID: 24100448
38. a novel function for Fli-1 in T cell development and leukaemogenesis PMID: 23667468
39. Podoplanin and Fli-1 immunohistochemistry may be useful in distinguishing atypical fibroxanthoma from angiosarcoma. PMID: 23518636
40. Ciprofloxacin has antifibrotic actions in Systemic sclerosis dermal and lung fibroblasts via the downregulation of Dnmt1, the upregulation of Fli1. PMID: 23041765
41. In 5q- syndrome, but not Diamond-Blackfan anemia, blood & bone marrow mononuclear cells had high Fli1 mRNA levels. This protects megakaryocytic cells from ribosomal stress & permits effective though dysplastic megakaryopoiesis. PMID: 22965552
42. Our findings of SNP array adn phenotype correclation do not support the traditional view that FLI1 deletion is the cause of thrombocytopenia in Jacobsen syndrome. PMID: 22887642
43. We found that transcriptional activation of PRKCB was directly regulated by the chimeric fusion oncogene EWSR1-FLI1 that drivesewing sarcoma growth. PMID: 22930730
44. FLI1 is a novel ETS transcription factor involved in gene fusions in prostate cancer and that intratumor genetic heterogeneity of ETS rearrangements can occasionally be found in index primary tumors. PMID: 22081504
45. Data show there are no differences in the distribution of immunohistochemical reactivity for CD31, CD34, D2-40, or FLI1 between AIDS-related and non-AIDS-related Kaposi sarcoma (KS) or between nodular- and patch/plaque-stage KS. PMID: 22372906
46. Expression of FLI-1, ELF-1, and GABP activated the PF4 promoter in HepG2 cells. PMID: 21931859
47. we have demonstrated association between FLI1 and susceptibility to CL caused by L. braziliensis. PMID: 21633373
48. FLI1 expression is frequently abnormal and prognostically adverse in acute myeloid leukemia. PMID: 21917756
49. a role of Fli1 as a negative regulator of the estrogen receptor alpha gene in dermal fibroblasts. PMID: 21451544
50. these results demonstrate that in SSc fibroblasts, c-Abl is an upstream regulator of the profibrotic PKCdelta/phospho-Fli-1 pathway, via induction of PKCdelta nuclear localization. PMID: 21321929
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相關(guān)疾病：

Ewing sarcoma (ES); Bleeding disorder, platelet-type 21 (BDPLT21)
亞細(xì)胞定位：

Nucleus.
蛋白家族：

ETS family
數(shù)據(jù)庫鏈接：

HGNC: 3749

OMIM: 193067

KEGG: hsa:2313

STRING: 9606.ENSP00000399985

UniGene: Hs.504281