Recombinant Human FACT complex subunit SSRP1 (SSRP1)

貨號：

CSB-YP022721HU
規格：
來源：

Yeast
其他：

貨號：

CSB-EP022721HU
規格：
來源：

E.coli
其他：

貨號：

CSB-EP022721HU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

貨號：

CSB-BP022721HU
規格：
來源：

Baculovirus
其他：

貨號：

CSB-MP022721HU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

SSRP1
Uniprot No.：

Q08945
別名：

FACT complex subunit SSRP1; Chromatin-specific transcription elongation factor 80 kDa subunit; Facilitates chromatin transcription complex 80 kDa subunit; FACT 80 kDa subunit; FACTp80; Facilitates chromatin transcription complex subunit SSRP1; Recombination signal sequence recognition protein 1; Structure-specific recognition protein 1; hSSRP1; T160; SSRP1 FACT80
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-709
氨基酸序列

AETLEFNDVYQEVKGSMNDGRLRLSRQGIIFKNSKTGKVDNIQAGELTEGIWRRVALGHGLKLLTKNGHVYKYDGFRESEFEKLSDFFKTHYRLELMEKDLCVKGWNWGTVKFGGQLLSFDIGDQPVFEIPLSNVSQCTTGKNEVTLEFHQNDDAEVSLMEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTPRGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKDEDISLTLNMNEEEVEKRFEGRLTKNMSGSLYEMVSRVMKALVNRKITVPGNFQGHSGAQCITCSYKASSGLLYPLERGFIYVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSSEDSASGSDE
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
基因功能參考文獻：
1. Data revealed that SSRP1 is highly overexpressed in glioma tissues at both the mRNA and protein levels and correlated with tumor grade. Further analysis demonstrated that that SSRP1 regulates the proliferation and metastasis of glioma cells via the MAPK signaling pathway. PMID: 29048646
2. These data uncover a previously unknown role for SSRP1 in promoting the activation of the Wnt signaling pathway activity during cellular differentiation. PMID: 27146025
3. we propose that FACT is both a marker and a target of aggressive breast cancer cells, whose inhibition results in their death or conversion a less aggressive subtype PMID: 28423528
4. High FACT expression is associated with glioblastoma. PMID: 27370399
5. The association of LEDGF proteins with the FACT complex and give further support to a role of SSRP1 in HIV-1 infection. PMID: 27216501
6. Results show that SSRP1 upregulation contributed to hepatocellular carcinoma development and the tumor-suppressive miR-497 served as its negative regulator. PMID: 26755331
7. FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres. PMID: 27284163
8. This discloses a novel property of FACT wherein it has a co-remodeling activity and strongly enhances the remodeling capacity of the chromatin remodelers. Altogether, our data suggest that FACT may acts in concert with RSC to facilitate excision of DNA lesions during the initial step of BER. PMID: 27467129
9. SSRP1/Ets-1/Pim-3 signalling is tightly associated with the proliferation, apoptosis, autophagy, invasion and clonogenicity of nasopharyngeal carcinoma cells, and blockage of this signalling facilitates chemosensitivity of the cells to docetaxel. PMID: 27525970
10. Our studies facilitate the understanding of SSRP1 and provide insights into the molecular mechanisms of interaction with DNA and histones of the FACT complex. PMID: 26687053
11. AID accesses the H2B N-terminal basic region exposed by partial unwrapping of the nucleosomal DNA, thereby triggering the invasion of FACT into the nucleosome PMID: 26966247
12. FACT is required for TOP1 binding to H3K4me3 at non-B DNA containing chromatin for the site-specific cleavage. PMID: 26842758
13. FACT, NF-kappaB, and cell-cycle progression are inhibited by quinacrine, which overcomes resistance to erlotinib in non-small cell lung cancer PMID: 25028470
14. EEF1A1, SSRP1, and XRCC6 are novel interacting partners of the mineralocorticoid receptor PMID: 25000480
15. A primary role for FACT in RNF20 recruitment chromatin remodeling for initiation of homologous recombination repair. PMID: 24357716
16. In the absence of FACT complex, SSRP1 and SPT16 mRNAs are unstable and inefficiently translated, making reactivation of FACT function unlikely in normal cells. PMID: 23839038
17. hFACT-H2A/H2B interactions play a key role in overcoming the nucleosomal barrier by Pol II and promoting nucleosome survival during transcription PMID: 23610384
18. SETD2 activity modulates FACT recruitment and nucleosome dynamics, thereby repressing cryptic transcription initiation. PMID: 23325844
19. The HSF1-RPA complex leads to preloading of RNA polymerase II and opens the chromatin structure by recruiting a histone chaperone, FACT PMID: 22940245
20. specific FACT subunits synchronize interactions with various target sites on individual nucleosomes to generate a high affinity binding event and promote reorganization. PMID: 21969370
21. DNA-PK and FACT both play roles in DNA repair. Therefore both are putative targets for therapeutic inhibition. PMID: 21679440
22. The histone chaperone FACT: structural insights and mechanisms for nucleosome reorganization. PMID: 21454601
23. Studies suggest that that even though FACT has rapid chromatin-binding activity, the binding pattern of FACT on chromatin changes after origin licensing, which may contribute to the establishment of its functional link to the DNA replication machinery. PMID: 21232560
24. results demonstrate that SSRP1 is crucial for microtubule growth and spindle assembly during mitosis. PMID: 19995907
25. SSRP1 stimulates p63 activity by associating with this activator at the promoter PMID: 12374749
26. CK2 regulates the DNA-binding ability of SSRP1 and that this regulation may be responsive to specific cell stresses. PMID: 15659405
27. These results demonstrate that SSRP1 degradation during apoptosis is a two-step process coupling caspase cleavage and ubiquitin-dependent proteolysis. PMID: 16498457
28. SSRP1 has Spt16-dependent and -independent roles in regulating gene transcription in human cells. PMID: 17209051
29. CENP-H-containing complex facilitates deposition of newly synthesized CENP-A into centromeric chromatin in cooperation with FACT and CHD1. PMID: 19625449
30. SSRP1 may play a role in the DNA damage response mediated by homologous recombination; SSRP1 physically interacts with a key recombination repair protein, Rad54 PMID: 19639603
31. These results indicate that SSRP1 is a novel cellular protein involved in LANA-dependent DNA replication. PMID: 19710137
32. occurrence of an unusual TG 3' splice site in intron 1 PMID: 17672918
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亞細胞定位：

Nucleus. Nucleus, nucleolus. Chromosome.
蛋白家族：

SSRP1 family
數據庫鏈接：

HGNC: 11327

OMIM: 604328

KEGG: hsa:6749

STRING: 9606.ENSP00000278412

UniGene: Hs.523680