Recombinant Human Elongation factor 1-alpha 1 (EEF1A1)

^{In
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中文名稱：

人EEF1A1重組蛋白
貨號：

CSB-EP007409HUa0
說明書：

MSDS Datasheet
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

EEF1A1
Uniprot No.：

P68104
別名：

CCS 3; CCS3; Cervical cancer suppressor 3; chunp6927; CTCL tumor antigen; EE1A1; EEF 1; EEF1A; eEF1A-1; EEF1A1; EF-1-alpha-1; EF-Tu; EF1A; EF1a like protein; EF1A1_HUMAN; Elongation factor 1 alpha subunit; Elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; Eukaryotic translation elongation factor 1 alpha 1; Eukaryotic translation elongation factor 1 alpha 1 like 14; Glucocorticoid receptor AF 1 specific elongation factor; GRAF 1EF; HNGC:16303; ik:tdsubc_2a3; ik:tdsubc_2b3; LENG7; Leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; Prostate tumor inducing protein 1; PTI1; tdsubc_2a3; Translation elongation factor 1 alpha 1 like 14; wu:fa91c07; wu:fa94b03; wu:fi13b09; xx:tdsubc_2a3; xx:tdsubc_2b3
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

54.1 kDa
表達區域：

1-462aa
氨基酸序列

MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The N-terminal 6xHis tag was fused to the gene fragment corresponding to the 1-462aa of the human EEF1A1 protein and then was cloned into an expression vector. The expression vector was transformed into the E.coli for expression. The generated product was purified and separated to obtain the recombinant human EEF1A1 protein. Its purity is higher than 85%. This recombinant EEF1A1 protein showed an apparent molecular weight of about 55 kDa under SDS-PAGE condition. It may find use in the research about epigenetics and nuclear signaling transduction.
In translation, EEF1A1 is partly responsible for the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In nuclear export of proteins, EEF1A1 plays a critical role. Additionally, EEF1A1 regulates the cell growth and proliferation in the mitotic apparatus formation, signal transduction or other biological process. In the heart and kidney, EEF1A1 as a co-activator of the mineralocorticoid receptors, EEF1A1 could influence the several genes expression like GILZ and SGK1. In some cases, EEF1A1 expression levels are associated with breast cancer and prostate cancer.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1.; (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.
基因功能參考文獻：
1. The functional studies of specific methylation sites found them to have distinct effects, notably on Eukaryotic elongation factor 1A (eEF1A)-related processes of translation and tRNA aminoacylation. PMID: 29398204
2. A strong correlation was found between the dysregulation of eEF1A synthesis and Alzheimer's Disease-associated synaptic failure. PMID: 27567813
3. the different kinases that recognize the Ser and Thr residues of the eEF1A1 and eEF1A2 isoforms and regulate their involvement in different cellular processes like cell survival and apoptosis. PMID: 27568183
4. Taken together, these findings enabled us to identify a novel mechanism by which eEF1A1 regulates the cell cycle's G1 phase to promote tumor proliferation by regulating cyclin D1 expression through STAT1 signaling in HCC. PMID: 29079187
5. Methylation of lysine (K36) in eukaryotic elongation factor alpha (eEF1A) proteins is dependent on EEF1A lysine methyltransferase 4 (eEF1A-KMT4) in vivo. PMID: 28520920
6. The present study identifies METTL21B as the enzyme responsible for methylation of eEF1A on Lys-165 and shows that this modification is dynamic, inducible and likely of regulatory importance. PMID: 28108655
7. EEF1A1 is somatically mutated in 9% of follicular lymphoma tumors. PMID: 25713363
8. we show how FAT10 stabilizes the translation elongation factor eEF1A1, which contributes to cancer cell proliferation PMID: 27312528
9. The expression of RPL13A and EEF1A1 was not affected by differentiation, thus being these genes the most stable candidates as reference genes for RT-PCR. PMID: 27304673
10. Study demonstrated that PAK4 interacted with eEF1A1 to promote migration and invasion of gastric cancer cells, thereby providing new insights into the function of PAK4 and eEF1A1 in the progression of gastric cancer. PMID: 28393218
11. this study demonstrates that eEF1A1 regulates the subcellular location of expanded poly(A) proteins and is therefore a potential therapeutic target for combating the pathogenesis of poly(A) diseases. PMID: 28246169
12. analysis of eEF1A1 oligomerization reveals that specific cysteine residues are required for this oligomerization activity PMID: 26515794
13. Data (including data from studies using purified proteins/hepatocyte lysates) suggest eEF1A1/Sgt1a interact as multimer; D2/D3 domains of eEF1A1 and TPR domain of Sgt1 are involved in multimer formation; Sgt1 competes with viral RNA to bind to eEF1A. PMID: 26545799
14. Data indicate that the methylation of lysine (Lys) in elongation factor 1A (eEF1A) by methyltransferase is conserved from yeast to human. PMID: 26545399
15. ROCK phosphorylated eEF1A1 is a novel substrate for TIMAP-PP1 underlining the complex regulatory role of TIMAP in the endothelium. PMID: 26497934
16. Study found reduced levels of hippocampal eEF1A protein in Alzheimer's disease PMID: 26551858
17. Our results provide novel information on the intracellular distribution and interaction of eEF1A isoforms. PMID: 26212729
18. These results suggest that the antitumor effects of paclitaxel in breast cancer are mediated by activation of the AMPK/EF1alpha/FOXO3a signaling pathway. PMID: 26397839
19. These results suggest that miR-33a-5p is downregulated during Japanese encephalitis virus infection, which contributes to viral replication by increasing the intracellular level of EEF1A1, an interaction partner of the viral NS3 and NS5 proteins. PMID: 26819305
20. Low expression of eEF1A1 was associated with cervical squamous cell carcinoma. PMID: 25893434
21. eEF1A-1 protein was induced by high palmitate, and partially re-localized from its predominant location at the ER to polymerized actin at the cell periphery, coinciding with the onset of ER stress. PMID: 26102086
22. The combined evidence indicates a direct interaction between eEF1A and reverse transcriptase is crucial for HIV reverse transcription and replication PMID: 26624286
23. eEF1A interacts with 5'UTR of HIV-1 genomic RNA and the interaction is important for late DNA synthesis in reverse transcription. PMID: 26242867
24. Data show that translation elongation factor eEF1A1 coordinates the heat shock response by adjusting transcriptional yield to translational needs. PMID: 25233275
25. EEF1A1, SSRP1, and XRCC6 are novel interacting partners of the mineralocorticoid receptor PMID: 25000480
26. eEF1A1 may mediate SAMHD1 turnover by targeting it to the proteosome for degradation through association with Cullin4A and Rbx1. PMID: 25423367
27. protein expressions of stathmin and EF1alpha were found in DNs of precancerous lesions, whereas they were absent or present at very low levels in normal liver and liver cirrhosis PMID: 24885363
28. These data provide the experimental evidence that telomere shortening and related inflammatory proteins are associated with human IgAN, and it could be a new direction for the disease progression study. PMID: 24903994
29. findings suggest that eEF1A contributes to the morphology of postsynaptic membrane specializations at inhibitory synapses PMID: 23839781
30. Sequence differences in the EF1alpha -3 promoter likely account for the activity differences seen. Investigators need to recognize that all promoters of the same name may not be equivalent in driving transgene expression. PMID: 24688302
31. Describes a nuclear role for eEF1A and provide a mechanism for protein nuclear export that attenuates the activity of SNAG-containing transcription factors PMID: 24209753
32. results reveal a novel molecular mechanism for a non-canonical role of eEF1A1 in signal transduction via direct modulation of kinase-dependent phosphorylation events PMID: 24487064
33. Human eEF1A1 is a negative regulator of the pro-apoptotic function of p53 and p73 PMID: 23799104
34. We propose that the reduction in SphK1 activity late in DENV-2-infected cells is a consequence of DENV-2 out-competing SphK1 for eEF1A binding and hijacking cellular eEF1A for its own replication strategy. PMID: 23939980
35. Knocking down eEF1A1 gene has noticeable effects on the proliferation inhibition and apoptosis induction of Jurkat cells. PMID: 22931638
36. eEF1A binds defective polypeptides released from ribosomes, which generates a signal that triggers aggresome formation. PMID: 22357952
37. This study identified eEF1A1 as a FAT10-specific binding protein, and when the expression of FAT10 was reduced by siRNA knockdown, this resulted in downregulation of eEF1A1 expression in hepatoma cells. PMID: 22569823
38. Overexpression of eukaryotic translation elongation factor 1 alpha 1 is associated with nonepithelial ovarian cancer. PMID: 22531302
39. Results show a new function of eEF1A that contributes to cell regulation, including anoikis. PMID: 22399298
40. In Huh-7 hepatoma cells, the hepatitis B virus X protein inhibits dimer formation of eEF1A1, hence blocks filamentous actin bundling. PMID: 22499008
41. High eEF1A1 is associated with metastatic progression of prostate cancer. PMID: 22355332
42. An immunoreactive protein detected in sera from 21 of 40 infiltrating ductal breast carcinoma patients was isolated and subsequently identified as elongation factor-Tu. PMID: 21704614
43. Mammalian PUM2-Ago-eEF1A inhibited translation of nonadenylated and polyadenylated reporter mRNAs in vitro. PMID: 22231398
44. Pilot evaluation in archive prostate tissues showed the presence of EEF1A2 mRNA in near all neoplastic and perineoplastic but not in normal samples or in benign adenoma; in contrast, EEF1A1 mRNA was everywhere detectable PMID: 22095224
45. A co-localization of SORBS2 and eEF1A was evidenced at level of plasma membrane, thus suggesting the involvement of eEF1A1 in novel key signal transduction complexes. PMID: 21689717
46. study defines the mechanism regulating eEF1A-mediated SK1 activation, and also establishes SK1 as being integral for PTI-1-induced oncogenesis PMID: 20838377
47. These findings provide evidence that elongation factor-1 alpha correlates closely with the survival of patients with prostate cancer and may be a novel prognostic factor. PMID: 20545466
48. Phosphorylation of eEF1A1 by TbetaR-I is a novel regulatory mechanism that provides a direct link to regulation of protein synthesis by TGF-beta, as an important component in the TGF-beta-dependent regulation of protein synthesis and cell proliferation PMID: 20832312
49. Data show that EF1alpha, RPL13a and YWHAZ are suitable genes for the RT-qPCR analysis and comparison of several sources of human MSC during in vitro characterization and differentiation as well as in an ex vivo animal model of global cerebral ischemia. PMID: 20716364
50. Proteomics was used to study colonic epithelial aging, for differential proteins in the human normal colonic epithelial tissues from young and old people. Rack1, EF-Tu and Rhodanese, three validated differential proteins, were further investigated. PMID: 20099848
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亞細胞定位：

Cytoplasm. Nucleus. Nucleus, nucleolus. Cell membrane.
蛋白家族：

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-Tu/EF-1A subfamily
組織特異性：

Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.
數據庫鏈接：

HGNC: 3189

OMIM: 130590

KEGG: hsa:1915

STRING: 9606.ENSP00000330054

UniGene: Hs.535192