Recombinant Human DNA helicase INO80 (INO80), partial

中文名稱：

人INO80重組蛋白
貨號：

CSB-YP892466HU
規格：
來源：

Yeast
其他：

中文名稱：

人INO80重組蛋白
貨號：

CSB-EP892466HU
規格：
來源：

E.coli
其他：

中文名稱：

人INO80重組蛋白
貨號：

CSB-EP892466HU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人INO80重組蛋白
貨號：

CSB-BP892466HU
規格：
來源：

Baculovirus
其他：

中文名稱：

人INO80重組蛋白
貨號：

CSB-MP892466HU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

INO80
Uniprot No.：

Q9ULG1
別名：

DNA helicase INO80; hINO80; homolog of yeast INO80; INO80; INO80 complex subunit A; INO80 homolog; INO80_HUMAN; INO80A; INOC1; KIAA1259; Putative DNA helicase INO80 complex homolog 1
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

ATPase component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and DNA repair. Binds DNA. As part of the INO80 complex, remodels chromatin by shifting nucleosomes. Regulates transcription upon recruitment by YY1 to YY1-activated genes, where it acts as an essential coactivator. Involved in UV-damage excision DNA repair. The contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Involved in DNA replication. Required for microtubule assembly during mitosis thereby regulating chromosome segregation cycle.
基因功能參考文獻：
1. human INO80 chromatin remodeling complex is more compositionally heterogenous at its genomic targets than anticipated. PMID: 29432129
2. structure of the human INO80 chromatin remodeller with a bound nucleosome, which reveals that INO80 interacts with nucleosomes in a previously undescribed manner PMID: 29643506
3. INO80-silencing MSC cultured in osteogenic condition expressed lower mRNA levels of osteoblast-specific genes, including Runx2, Osx, Col1alpha1 and OCN. INO80 can interact with Wdr5 in MSC and positively regulates the canonical Wnt signaling transduction. PMID: 27804957
4. Instead nucleosome sliding requires cooperativity between two INO80 complexes that monitor DNA length simultaneously on either side of the nucleosome during sliding. The C-terminal domain of the human Ino80 subunit (Ino80CTD) binds cooperatively to DNA and dimerisation of these domains provides crosstalk between complexes. PMID: 28585918
5. Ino80 was upregulated in cervical cancer and promoted cell proliferation and tumorigenesis by binding to the Nanog transcription start site and enhancing its expression. PMID: 27750218
6. By demonstrating a key role for SUMOylation in regulating the INO80 chromatin remodeling complex, this work illustrates the power of bioinformatics analysis of large data sets in predicting novel biological phenomena. PMID: 28254775
7. Our work defines SETD2 as a tumor suppressor gene in Hepatosplenic T-cell lymphoma (HSTL)and implicates genes including INO80 and PIK3CD in the disease PMID: 28122867
8. our study defines a critical role of INO80 in promoting oncogenic transcription and NSCLC tumorigenesis, and reveals a potential treatment strategy for inhibiting the cancer transcription network by targeting the INO80 chromatin remodeling complex. PMID: 27641337
9. Interactions of the chromatin remodeling protein INO80 with DNA, and the role of YY1 in this process have been described. PMID: 27428271
10. The inositol hexaphosphate binding site is located within the C-terminal region of the Ino80 subunit. PMID: 27257055
11. the existence of both INO80 and YY1 is required for recruiting the INO80/YY1 complex to BCCIP promoter region. Our findings strongly indicate that BCCIP is a potential target gene of the INO80/YY1 complex. PMID: 27535137
12. The expression of Ino80, the SWI/SNF ATPase, is elevated in melanoma cells and patient melanomas compared with normal melanocytes and benign nevi. PMID: 27340176
13. Overexpression of miRNA-148a and knockdown of INO80 acted synergistically to decrease the expression of stem cell marker genes as well as to attenuate stem cell-specific properties including the ability to form tumors. PMID: 27779717
14. INO80 complex negatively regulates p21 expression in a p53-mediated mechanism and is implicated in cell cycle phase G2/M arrest and abnormal chromosome stability. PMID: 26340092
15. INO80 is stabilized and targeted to replication forks by BAP1 during normal DNA synthesis but downregulated in BAP1 defective cancer cells PMID: 25283999
16. Mutations in INO80D is associated with translocation renal cell carcinoma. PMID: 24899691
17. The data indicate that under conditions of replication stress INO80 protects stalled forks from collapsing and allows their subsequent restart. PMID: 25016522
18. human Inositol auxotrophy 80 (Ino80) SNF2 ATPase is subject to regulation at multiple levels in the INO80 chromatin-remodeling complex. PMID: 24297934
19. These results suggest that the human INO80 complex, like the yeast complex, was involved in the DNA damage tolerance pathway and that phosphorylation of human INO80 was involved in the DNA damage tolerance pathway. PMID: 22166198
20. These results suggest that hIno80 can play a direct role in the spindle assembly independent of its chromatin remodeling activity. PMID: 22133677
21. Ino80 mediates double-strand break repair through its role in DNA end strand resection PMID: 21947284
22. Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. PMID: 21303910
23. These results suggest that hINO80 assists double-strand break repair by positively regulating the expression of the Rad54B and XRCC3 genes. PMID: 20687897
24. hINO80 associates with spindle microtubule during mitosis, and its deficiency leads to defective microtubule assembly and abnormal chromosome segregation. PMID: 20237820
25. report the cloning, expression, and functional activity of the domains from hINO80 gene both in terms of the DNA dependent ATPase as well as DNA binding activity PMID: 16298340
26. Methods for purification and assay of the INO80 chromatin remodeling complexes are described. PMID: 17101442
27. Binding of YY1 to its DNA sites in target genes requires INO80, suggesting that YY1 uses the INO80 complex not only to activate transcription but also to gain access to target promoters PMID: 17721549
28. Ino80 has a crucial role in stabilizing a stalled replisome to ensure proper restart of DNA replication PMID: 18376411
29. INO80 is an important new player in DNA replication to help safeguard the integrity of the genome PMID: 18388905
30. In the nucleus Uch37 is also associated with the human Ino80 chromatin-remodeling complex (hINO80). PMID: 18922472
31. Identifies INOC1 gene product as a SNF2-like helicase associated with human INO80 chromatin remodeling complex (hINO80). hINO80 includes orthologs of 8 subunits of the yeast INO80 complex, as well as additional, metazoan-specific proteins. PMID: 16230350
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亞細胞定位：

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome.
蛋白家族：

SNF2/RAD54 helicase family
組織特異性：

According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas.
數據庫鏈接：

HGNC: 26956

OMIM: 610169

KEGG: hsa:54617

STRING: 9606.ENSP00000355205

UniGene: Hs.292949