Recombinant Human Cytotoxic granule associated RNA binding protein TIA1 (TIA1)

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中文名稱：

Recombinant Human Cytotoxic granule associated RNA binding protein TIA1 (TIA1)
品名簡稱：

Recombinant Human TIA1 protein
貨號：

CSB-EP023527HU
說明書：

MSDS Datasheet
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

TIA1
Uniprot No.：

P31483
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

49.0 kDa
表達區域：

1-386aa
氨基酸序列

MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAAAALAAMNGRKIMGKEVKVNWATTPSSQKKDTSSSTVVSTQRSQDHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDMINPVQQQNQIGYPQPYGQWGQWYGNAQQIGQYMPNGWQVPAYGMYGQAWNQQGFNQTQSSAPWMGPNYGVQPPQGQNGSMLPNQPSGYRVAGYETQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
基因功能參考文獻：
1. The repression of TIA-1 by miR-487a promoted cell proliferation and suppressed cell apoptosis in vitro, and the knockdown of miR-487a had the opposite effects. PMID: 30144499
2. TIA1 mutation is an uncommon genetic cause for ALS in the Chinese population. PMID: 29773329
3. TIA1 LCD mutations are not common in Chinese ALS/ALS-FTD PMID: 29699721
4. TIA1 is a novel causative gene of amyotrophic lateral sclerosis. PMID: 29370934
5. The purpose of this report is to provide a detailed description of the clinical and neuropathological features associated with the recently identified TIA1 mutations that cause ALS +/- FTD. PMID: 29216908
6. expressions of TIA-1 and MFF were augmented in the cancerous liver tissues compared to the corresponding non-tumor tissues at mRNA and protein level, while the levels of miR-200a-3p and miR-27a/b were relatively lower in the cancerous liver tissues PMID: 29496454
7. YAP (Yes-associated protein) expression negatively regulates TIA1 (Rox8 ortholog) expression and cell invasion in human cancer cells. PMID: 28174264
8. Downregulation of TIA-1-enhanced mitochondrial elongation, whereas ectopic expression of TIA-1 resulted in mitochondria fragmentation. In addition, TIA-1 increased mitochondrial activity, including the rate of ATP synthesis and oxygen consumption. PMID: 27612012
9. findings uncover a novel oncogenic function of TIA1 in esophageal tumorigenesis PMID: 26958940
10. studied a novel Amyotrophic Lateral Sclerosis/Frontotemporal Dementia (ALS/FTD) family and identified the P362L mutation in the low-complexity domain (LCD) of TIA1; genetic association analyses showed an increased burden of TIA1 LCD mutations in ALS patients compared to controls; TIA1 mutations significantly increased the propensity of TIA1 protein to undergo phase transition PMID: 28817800
11. Here we designed UC-rich and CU-rich 10-nt sequences for engagement of both RRM2 and RRM3 and demonstrated that the TIA-1 RRM23 construct preferentially binds the UC-rich RNA ligand. Together our data support a specific mode of TIA-1 RRM23 interaction with target oligonucleotides consistent with the role of TIA-1 in binding RNA to regulate gene expression PMID: 28184449
12. miR-19a could promote cell proliferation and migration in CRC cells and accelerated tumor growth in xenograft mice by targeting TIA1. PMID: 28257633
13. Data suggest that TPD52 (tumor protein D52) and a TPD52 fragment (residues 78-280) along with TIA-1 (T-cell intracellular antigen-1) and TIAR (TIA-1-related protein) contribute to mRNA stability as cis-acting and trans-acting factors; 3prime-untranslated regions of TPD52, TPD53, and TPD54 regulate expression of their respective genes in a post-transcriptional manner by altering mRNA stability. PMID: 28298474
14. The results provide a mechanism for exon 16 3' splice site activation in which a coordinated effort among TIA1, Pcbp1, and RBM39 stabilizes or increases U2 snRNP recruitment, enhances spliceosome A complex formation, and facilitates exon definition through RBM39-mediated splicing regulation. PMID: 28193846
15. AT1R mRNA is regulated by TIA-1 in a ER stress-dependent manner. PMID: 26681690
16. This study showed that reactive oxygen species such as H2O2 oxidize the cytoplasmic stress granules (SG)-nucleating protein TIA1, thereby inhibiting SG assembly. PMID: 26738979
17. TIA proteins play a role in the regulation and/or modulation of cellular homeostasis related to focal/cell adhesion, extracellular matrix and membrane and cytoskeleton dynamics. PMID: 25405991
18. SERPINE1 mRNA dissociates from the translational repressor proteins Ago2 and TIA-1 upon platelet activation PMID: 25673011
19. Alternative splicing of TIA-1 in human colon cancer regulates VEGF isoform expression, angiogenesis, tumour growth and bevacizumab resistance. PMID: 25224594
20. results suggest that TIA-1 and TIAR are two new host factors that interact with 5-UTR of EV71 genome and positively regulate viral replication PMID: 26363455
21. TIA1 inhibition of the exon 8 exclusion led to a decrease in SIRT1-Exon8 mRNA levels. PMID: 24566137
22. TIA proteins can function as long-term regulators of the ACTB mRNA metabolism in mouse and human cells. PMID: 24766723
23. TIA1-knockdown HeLa cells show an increase in ribosomes and translational machinery components. PMID: 24927121
24. Structural insights into the role of binding avidity and the contributions of the TIA-1 RNA recognition motifs for recognition of pyrimidine-rich RNAs. PMID: 24682828
25. TIA1 gene expression do not predict prognosis in patients diagnosed with cutaneous T-cell lymphoma. PMID: 24433873
26. Welander distal myopathy is caused by mutated TIA1 through a dominant pathomechanism probably involving altered stress granule dynamics. PMID: 23401021
27. Work described here examined the punctate pattern of SRp20 localization in the cytoplasm of poliovirus-infected cells, demonstrating the partial co-localization of SRp20 with the stress granule marker protein TIA-1. PMID: 23830997
28. RNA binding mediated by either isolated RRM3 or the RRM23 construct is controlled by slight environmental pH changes due to the protonation/deprotonation of TIA-1 RRM3 histidine residues. PMID: 23902765
29. the TIA1 mutation causes perturbed RNA splicing and cellular stress resulting in WDM. PMID: 23348830
30. Three RNA recognition motifs participate in RNA recognition and structural organization by the pro-apoptotic factor TIA-1. PMID: 22154808
31. Results characterise the C-terminal RRM2 and RRM3 domains of T-cell intracellular antigen-1 protein. PMID: 21846467
32. A role for TIA proteins as growth/tumour-suppressor genes. PMID: 21284605
33. TIA-1 cytotoxic granule-associated RNA binding protein has a role in preventing progression of mismatch repair-proficient colorectal cancer PMID: 21179245
34. TAR DNA-binding protein 43 (TDP-43) regulates stress granule dynamics via differential regulation of G3BP and TIA-1. PMID: 21257637
35. TIA1 and TIAR proteins are intron-associated positive regulators of SMN2 exon 7 splicing. PMID: 21189287
36. Severe hypoxia caused co-aggregation of TIAR/TIA-1 and these proteins suppressed HIF-1alpha expression. PMID: 20980400
37. Data show that TIA1 and TIAL1 bind at the same positions on human RNAs, and are consistent with a model where TIA proteins shorten the time available for definition of an alternative exon by enhancing recognition of the preceding 5' splice site. PMID: 21048981
38. Data show that apoptotic (TIAR and TIA-1) marker expression in thyroid tissues from adolescents with immune thyroid diseases is higher than in non-immune thyroid diseases. PMID: 20675271
39. Down-regulation of the IGFBP-3 transcripts correlated with the up-regulation of the TIA-1 transcripts in primary HCC biopsies. PMID: 20599318
40. Sam68 is recruited into stress granules through complexing with TIA-1 in response to oxidative stress PMID: 19615357
41. apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing PMID: 11106748
42. Data suggest that TIA-1 functions as a translational silencer of cyclooxygenase-2 (COX-2) expression and support the hypothesis that dysregulated RNA-binding of TIA-1 promotes COX-2 expression in neoplasia. PMID: 12885872
43. Increased TIA-1 gene expression is associated with sensitize endothelial cells to proapoptotic stimuli present in the tumor microenvironment and enhance NK cell cytotoxic activity against cancer cells in advanced soft tissue sarcoma PMID: 12949814
44. data indicate that, in CFTR exon 9, TIA-1 binding to the polypyrimidine-rich controlling element recruits U1 small nuclear ribonucleoprotein to the weak 5'-ss and induces exon inclusion PMID: 14966131
45. activated during HSV-1 infection and accumulated in cytoplasm of cells 6 hr after infection PMID: 15280467
46. TIA-1 represses the translation of target transcripts. PMID: 16227602
47. Results describe the gene expression of tristetraprolin, T-cell intracellular antigen and Hu antigen R in synovial tissues from rheumatoid arthritis and osteoarthritis patients. PMID: 16820934
48. FAST K synergizes with TIA-1/TIAR proteins to regulate Fas alternative splicing PMID: 17135269
49. TIAR regulates the relative expression of TIA-1 isoforms. PMID: 17488725
50. Cytotoxic molecule (CM) expression, specifically TIA1 and granzyme B, is predictive of prognosis in Hodgkin's-like anaplastic large cell lymphoma. PMID: 17493234
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相關疾病：

Welander distal myopathy (WDM)
亞細胞定位：

Cytoplasm, Stress granule. Nucleus.
數據庫鏈接：

HGNC: 11802

OMIM: 603518

KEGG: hsa:7072

STRING: 9606.ENSP00000401371

UniGene: Hs.413123