Recombinant Human Cytochrome P450 2A13 (CYP2A13)

品名簡稱：

Recombinant Human CYP2A13 protein
貨號：

CSB-EP621969HU
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

CYP2A13
Uniprot No.：

Q16696
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

64.1 kDa
表達區域：

1-494aa
氨基酸序列

MLASGLLLVTLLACLTVMVLMSVWRQRKSRGKLPPGPTPLPFIGNYLQLNTEQMYNSLMKISERYGPVFTIHLGPRRVVVLCGHDAVKEALVDQAEEFSGRGEQATFDWLFKGYGVAFSNGERAKQLRRFSIATLRGFGVGKRGIEERIQEEAGFLIDALRGTHGANIDPTFFLSRTVSNVISSIVFGDRFDYEDKEFLSLLRMMLGSFQFTATSTGQLYEMFSSVMKHLPGPQQQAFKELQGLEDFIAKKVEHNQRTLDPNSPRDFIDSFLIRMQEEEKNPNTEFYLKNLVMTTLNLFFAGTETVSTTLRYGFLLLMKHPEVEAKVHEEIDRVIGKNRQPKFEDRAKMPYTEAVIHEIQRFGDMLPMGLAHRVNKDTKFRDFFLPKGTEVFPMLGSVLRDPRFFSNPRDFNPQHFLDKKGQFKKSDAFVPFSIGKRYCFGEGLARMELFLFFTTIMQNFRFKSPQSPKDIDVSPKHVGFATIPRNYTMSFLPR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

13-23 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Exhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N-dimethylaniline, 2'-methoxyacetophenone, N-nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity.
基因功能參考文獻：
1. CYP2A13 is the most efficient enzyme to metabolize N'-Nitrosonornicotine in vitro. PMID: 27567546
2. The results of this case-control study suggested that the CYP2A13*1/*2 genotype decreased the risk of susceptibility to bladder cancer in Japanese smokers PMID: 27725446
3. CYP2A13 levels in human lungs can be suppressed by disease-associated inflammation in tissue donors. PMID: 25710941
4. an etiologically relevant interaction between CYP2A13 and ABCB1 associated with lung cancer was analysed PMID: 25667220
5. The transgenic CYP2A13 is active. PMID: 24907355
6. CYP2A13 played a critical role in AFB1-induced neoplastic transformation. PMID: 24114584
7. Data suggest important role of CYP2A13 in AFG1- (aflatoxin G1)-induced cytotoxicity, DNA damage, and activation of signal pathways in bronchial epithelium; data suggest similarities in CYP2A13-catalyzed bioactivation of AFG1 and AFB1 (aflatoxin B1). PMID: 23907605
8. CYP2A13 is efficient in 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone bioactivation in vivo and it can mediate 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesis. PMID: 23917075
9. Findings suggest the potential involvement of pulmonary CYP2A13 in the early occurrence of NSCLC as well as in the development of EGFR gene mutations. PMID: 23752126
10. an efficient approach for expressing functionally characterized, highly active and homogeneous CYP2A13 proteins. PMID: 23463547
11. CYP2A13 plays an important role in low-concentration AFB1-induced DNA damage, possibly linking environmental airborne AFB1 to genetic injury in the human respiratory system. PMID: 23583631
12. Findings suggest that CYP2A13 plays an important role in low-concentration AFB1-induced DNA damage, possibly linking environmental airborne AFB1 to genetic injury in human respiratory system. PMID: 23602888
13. The differential distribution pattern of CYP2A13 in the respiratory tract, which is of importance in considering the pulmonary susceptibility to carcinogens and the following lung cancer development, is detailed. PMID: 22890016
14. Tissue distribution of transgenic mRNA expression agrees well with the known respiratory tract-selective expression of CYP2A13 and CYP2F1 and hepatic expression of CYP2B6 in humans. PMID: 22397853
15. Report selective expression of CYP2A13 in human pancreatic alpha-islet cells. PMID: 22798551
16. Although both the hepatic CYP2A6 and respiratory CYP2A13 enzymes metabolize these compounds, CYP2A13 does so with much higher catalytic efficiency, but the structural basis for this has been unclear PMID: 22700965
17. The distribution frequencies of all eight known CYP2A13 missense alleles were examined in a Chinese Han population. PMID: 22315333
18. present results provide the first direct in vitro evidence demonstrating the predominant roles of CYP2A13 in 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced mutagenesis, possibly via metabolic activation of NNK alpha-hydroxylation PMID: 21473878
19. single nucleotide polymorphisms within the CYP2A13 gene affect metabolism of 5-methoxypsoralen in humans. PMID: 20798279
20. 2 novel polymorphisms T478C and T494C in CYP2A13 gene were associated with significantly reduced risk of cancer. CYP2A13 haplotype carrying variant alleles of T478C/T494C found associated with reduced risk of head/neck cancer. PMID: 20534012
21. significant interaction between smoking and methylation status of CYP2A13 in head and neck cancer. PMID: 20846153
22. The mechanisms underlying the decreased allelic expression of a common CYP2A13 allele (7520C>G) in the human lung, were investigated. PMID: 20431511
23. Observed clear prevalence of the variant CYP2A13 alelle causing premature stop at codon 101 (knockout allele *7) among the controls in comparison with the pancreatic cancer patients. PMID: 19812523
24. genetic polymorphism: identification of single-nucleotide polymorphisms and functional characterization of an Arg257Cys variant PMID: 12130698
25. CYP2A13 allele is associated with reduced risk of lung adenocarcinoma, suggesting the role of NNK-CYP2A13 interaction as a causative factor for the cancer. PMID: 14633739
26. In order to investigate the extent of CYP2A13 genetic polymorphism in a French Caucasian population of 102 individuals, a screening for sequence variations in the 5'-untranslated and protein encoding regions of its gene was performed. PMID: 15063809
27. The CYP2A13 Arg257Cys variant represents a common polymorphism in Chinese, with the 257Cys allele frequency being similar to the Hispanic and Asian groups, but significantly lower than in blacks PMID: 15115698
28. CYP2A13 mutant proteins showed a significant decrease in the catalytic efficiency for NNK alpha-hydroxylation PMID: 15333516
29. structure of CYP2A13 was determined to 2.35A by x-ray crystallography and compared with structures of CYP2A6. PMID: 17428784
30. the reported association of the CYP2A13(*)2 allele with decreased incidences of lung adenocarcinoma in smokers can be at least partly explained by a decrease in CYP2A13 function PMID: 18669584
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亞細胞定位：

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
蛋白家族：

Cytochrome P450 family
組織特異性：

Expressed in liver and a number of extrahepatic tissues, including nasal mucosa, lung, trachea, brain, mammary gland, prostate, testis, and uterus, but not in heart, kidney, bone marrow, colon, small intestine, spleen, stomach, thymus, or skeletal muscle.
數據庫鏈接：

HGNC: 2608

OMIM: 608055

KEGG: hsa:1553

STRING: 9606.ENSP00000332679

UniGene: Hs.567252