Recombinant Human Chromobox protein homolog 1 (CBX1)

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中文名稱：

Recombinant Human Chromobox protein homolog 1 (CBX1)
貨號(hào)：

CSB-EP004597HU
說明書：

MSDS Datasheet
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

CBX1
Uniprot No.：

P83916
別名：

HP1Hsbeta;Heterochromatin protein 1 homolog beta;HP1 beta;Heterochromatin protein p25;M31;Modifier 1 protein;p25beta
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

26.4 kDa
表達(dá)區(qū)域：

1-185aa
氨基酸序列

MGKKQNKKKVEEVLEEEEEEYVVEKVLDRRVVKGKVEYLLKWKGFSDEDNTWEPEENLDCPDLIAEFLQSQKTAHETDKSEGGKRKADSDSEDKGEESKPKKKKEESEKPRGFARGLEPERIIGATDSSGELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPSEDDDKKDDKN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.
基因功能參考文獻(xiàn)：
1. the prolyl residue of the PXXVXL motif appears to play a role distinct from that of Pro in the known HP1beta CSD-PXVXL complexes. PMID: 28223359
2. Data show that heterochromatin protein 1-beta (HP1beta) is a prototypic HP1 protein exemplifying most basal chromatin binding and effects. PMID: 27090491
3. increased HP1beta expression is associated with the poor prognosis in breast cancer PMID: 25769025
4. In this review, we summarize the current knowledge about targeting and functional mechanisms of PRCs, emphasizing the recent breakthroughs related to CBX proteins under a number of physiological and pathological conditions. PMID: 24793759
5. Data suggest the reduction of Heterochromatin Protein 1 isoforms HP1beta followed by a decrease in HP1alpha contributes to the pathogenesis of thyroid carcinomas, and their loss is a potential marker of thyroid malignancy and metastatic potential, respectively. PMID: 24840329
6. Phosphorylation at Ser89 and Ser175 results in localized conformational changes in HP1beta that do not compromise the ability of the protein to bind chromatin. PMID: 24561199
7. HP1 isoforms distinctly augment AR signaling and cell growth in prostate cancer. Therefore, silencing of HP1beta and HP1gamma may be a promising therapeutic strategy for treatment of prostate cancer. PMID: 23536649
8. The structural plasticity of HP1beta supports its ability to bind and connect a wide variety of binding partners in epigenetic processes. PMID: 23585859
9. HP1beta which is associated with PCNA in regions of DNA repair, is bound and does not exchange with the mobile pool, suggesting that HP1beta in association with PCNA may be a component of a DNA repair complex. PMID: 23337132
10. The HP1beta interactome is enriched with Lys methylated proteins.Role of HP1beta in DNA damage response. PMID: 23707759
11. study finds that while HP1apha and beta stimulate homologous recombination and single-strand annealing, HP1gamma has an inhibitory role PMID: 23287531
12. Methylation of lysine 9 in histone H3 directs alternative modes of highly dynamic interaction of heterochromatin protein hHP1beta with the nucleosome PMID: 22815475
13. HP1 beta and PCNA, a key player in DNA replication, are closely spaced components of a multiprotein complex involved in replication, both in S phase and during DNA repair, and that the functional complex requires formation of an HP1 dimer. PMID: 22617335
14. Abrogating GRP/GRPR signaling specifically down-regulates HP1(Hsbeta) expression and inhibiting GRPR signaling, or ablating HP1(Hsbeta) expression, increases colon cancer cell invasiveness in vitro. PMID: 21281799
15. HP1beta is involved in the proliferation of AR-expressing prostate cancer cells and progression to CRPC as an AR coactivator. PMID: 20308360
16. Isoform-specific interaction of HP1 with human TAFII130. PMID: 11959914
17. Gene regulation by human orthologs of Drosophila heterochromatin protein 1. PMID: 12054505
18. the chromodomain and the chromoshadow domain of HP1 are both required for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin PMID: 12560555
19. cells are exquisitely sensitive to the amount of HP1(Hsalpha) or HP1(Hsbeta) present, as their overexpression influences telomere stability, population doubling time, radioresistance, and tumorigenicity PMID: 14585993
20. HP1beta recruits endogenous HP1alpha to the chromatin and this induces heterochromatin formation and enhanced histone lysine methylation. PMID: 15899859
21. analysis of methylation-independent binding to histone H3 and cell cycle-dependent incorporation of HP1beta into heterochromatin PMID: 16547356
22. HP1 binding as analyzed through the crystal structure of the HP1-EMSY complex PMID: 16615912
23. Overexpression of HP1-BETA in eye imaginal discs caused abnormally patterned eyes, with reduced numbers of ommatidia. PMID: 17575412
24. HP1 recruitment-release is a sequential mechanism that is precisely regulated and highly dependent on transcription. PMID: 18239689
25. findings reveal an unrecognized signalling cascade that helps to initiate the DNA damage response, altering chromatin by modifying a histone-code mediator protein, HP1, but not the code itself PMID: 18438399
26. when HP1beta was co-expressed with TIF1beta/S473A, but not TIF1beta/S473E, the colocalization of TIF1beta/S473A and HP1beta to the promoters of Cdc2 and Cdc25A was enhanced. PMID: 18590578
27. a nonconventional interaction between HP1 and a tissue-specific transcription factor, MyoD. In addition, they strongly suggest that HP1 isoforms play important roles during muscle terminal differentiation in an isoform-dependent manner. PMID: 18599480
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亞細(xì)胞定位：

Nucleus. Note=Unassociated with chromosomes during mitosis.
組織特異性：

Expressed in all adult and embryonic tissues.
數(shù)據(jù)庫鏈接：

HGNC: 1551

OMIM: 604511

KEGG: hsa:10951

STRING: 9606.ENSP00000225603

UniGene: Hs.77254