Recombinant Human Bifunctional glutamate/proline--tRNA ligase (EPRS), partial

中文名稱：

人EPRS重組蛋白
貨號：

CSB-YP007747HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人EPRS重組蛋白
貨號：

CSB-EP007747HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人EPRS重組蛋白
貨號：

CSB-EP007747HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人EPRS重組蛋白
貨號：

CSB-BP007747HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人EPRS重組蛋白
貨號：

CSB-MP007747HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

EPRS
Uniprot No.：

P07814
別名：

Bifunctional aminoacyl tRNA synthetase; Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline tRNA ligase; Cell proliferation-inducing gene 32 protein; DKFZp313B047; EARS; Eprs; GLNS; Glu pro tRNA synthetase ; GLUPRORS; GluRS; Glutamate tRNA ligase; Glutamatyl prolyl tRNA synthetase; Glutaminyl tRNA synthetase; Glutamyl prolyl tRNA synthetase; Glutamyl tRNA synthetase; Glutamyl-tRNA synthetase; PARS; PIG 32; PIG32; Proliferation inducing gene 32 protein; Proliferation inducing protein 32; Proline tRNA ligase; Proline--tRNA ligase; Prolyl tRNA synthetase ; Prolyl-tRNA synthetase; QARS; QPRS; SYEP_HUMAN
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. The phosphorylation of EPRS1, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS1 function from protein synthesis to translation inhibition. Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan.
基因功能參考文獻：
1. five different EPRS mutations were identified. PMID: 29576217
2. Data indicate the necessity of EPRS for proliferation of tamoxifen-resistant estrogen receptor (ER+) breast cancer, but not ER- breast cancer cells. PMID: 27612429
3. analysis of the heterotetrameric complex structure of the glutathione transferase (GST) domains shared among the four MSC components, methionyl-tRNA synthetase (MRS), glutaminyl-prolyl-tRNA synthetase (EPRS), AIMP2 and AIMP3 PMID: 26472928
4. Dynamic model simulations predicted an inhibitory GAIT-element-interacting factor to account for this relationship and led to the identification of a truncated form of EPRS, a GAIT constituent that mediates binding to target transcripts. PMID: 22386318
5. Study reveals a unique role of Cdk5/p35 in activation of the major noncanonical function of EPRS, namely translational control of macrophage inflammatory gene expression. PMID: 21220307
6. Results show that glutamyl-prolyl-tRNA synthetase has a regulated, noncanonical activity that blocks synthesis of a specific protein. PMID: 15479637
7. Essentiality of this enzyme's domains in its noncanonical function of regulating inflammatory gene expression. PMID: 18374644
8. EPRS phosphorylation events regulate GAIT-mediated gene silencing. PMID: 19647514
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亞細胞定位：

Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
蛋白家族：

Class-I aminoacyl-tRNA synthetase family, Glutamate--tRNA ligase type 2 subfamily; Class-II aminoacyl-tRNA synthetase family
數據庫鏈接：

HGNC: 3418

OMIM: 138295

KEGG: hsa:2058

STRING: 9606.ENSP00000355890

UniGene: Hs.497788