Recombinant Human Arylacetamide deacetylase (AADAC), partial

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中文名稱：

Recombinant Human Arylacetamide deacetylase(AADAC),partial
貨號：

CSB-EP001014HU
說明書：

MSDS Datasheet
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP001014HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) AADAC.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP001014HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) AADAC.
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產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

AADAC
Uniprot No.：

P22760
別名：

AAAD_HUMAN; Aada; Aadac; Arylacetamide deacetylase (esterase); Arylacetamide deacetylase; CES5A1; DAC
種屬：

Homo sapiens (Human)
蛋白長度：

partial
來源：

E.coli
分子量：

63.1kDa
表達區域：

24-399aa
氨基酸序列

PDNVEEPWRMMWINAHLKTIQNLATFVELLGLHHFMDSFKVVGSFDEVPPTSDENVTVTETKFNNILVRVYVPKRKSEALRRGLFYIHGGGWCVGSAALSGYDLLSRWTADRLDAVVVSTNYRLAPKYHFPIQFEDVYNALRWFLRKKVLAKYGVNPERIGISGDSAGGNLAAAVTQQLLDDPDVKIKLKIQSLIYPALQPLDVDLPSYQENSNFLFLSKSLMVRFWSEYFTTDRSLEKAMLSRQHVPVESSHLFKFVNWSSLLPERFIKGHVYNNPNYGSSELAKKYPGFLDVRAAPLLADDNKLRGLPLTYVITCQYDLLRDDGLMYVTRLRNTGVQVTHNHVEDGFHGAFSFLGLKISHRLINQYIEWLKENL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

E.coli expressed Recombinant Human Arylacetamide deacetylase(AADAC) is a partial-length protein containing 376 amino acids. It carries a 10xHis-SUMO-tag at the N-terminus and a Myc-tag at the C-terminus. Its expression region is the 24-399aa of the human AADAC protein. The LC-MS/MS Analysis validated its identity and SDS-PAGE determined its purity. Its purity is greater than 90%. This protein is in-stock so that it will reach your lab bench faster. In addition to serving as an immunogen for antibody synthesis, the recombinant AADAC protein may also be used to investigate whether it is involved in the breakdown of certain drugs.

AADAC is a serine hydrolase that widely catalyzes the hydrolysis of some clinically used drugs. AADAC is primarily expressed in the human intestine and liver and is responsible for the hydrolysis of flutamide, phenacetin, and rifampicin. It is closely related to kidney failure and hepatotoxicity in some patients after taking flutamide or phenacetin.
Datasheet & COA：

Please contact us to get it.

引用文獻

Predominant contributions of carboxylesterase 1 and 2 in hydrolysis of anordrin in humans Jiang J.et al,Xenobiotica,2017

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靶點詳情

功能：

Displays cellular triglyceride lipase activity in liver, increases the levels of intracellular fatty acids derived from the hydrolysis of newly formed triglyceride stores and plays a role in very low-density lipoprotein assembly. Displays serine esterase activity in liver. Deacetylates a variety of arylacetamide substrates, including xenobiotic compounds and procarcinogens, converting them to the primary arylamide compounds and increasing their toxicity.
基因功能參考文獻：
1. that the AADAC gene c.361+1G>A variant may be a potential candidate factor for Tourette syndrome development PMID: 29253601
2. Data suggest that AADAC in hepatocyte microsomes hydrolyzes ketoconazole, a synthetic imidazole antifungal agent, to N-deacetyl ketoconazole and thus triggers hepatocellular toxicity. PMID: 27422753
3. AADAC deletion is a candidate susceptibility factor for Gilles de la Tourette Syndrome PMID: 26444075
4. Translational N-glycosylation of AADAC plays a crucial role in regulating AADAC enzyme activity. PMID: 24125761
5. Lipolysis of cellular TG and VLDL production were impaired in HCV infected cells during the early peak of viral infection. This was partially explained by an apparent deficiency of a putative TG lipase, arylacetamide deacetylase (AADAC). PMID: 23542347
6. An AADAC*3 allele yields decreased enzyme activity in liver microsomes. PMID: 22415931
7. human AADAC was the enzyme responsible for the deacetylation of rifamycins and would affect the induction rate of drug-metabolizing enzymes by rifamycins and their induced hepatotoxicity. PMID: 21856291
8. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
9. two intrinsic endoplasmic reticulum (ER) proteins, 11beta-hydroxysteroid dehydrogenase, isozyme 1 (11beta-HSD) and the 50-kDa esterase (E3), sharing some amino acid sequence motifs in their N-terminal transmembrane (TM) domains. PMID: 10318829
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亞細胞定位：

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Microsome membrane; Single-pass type II membrane protein.
蛋白家族：

'GDXG' lipolytic enzyme family
組織特異性：

Detected in liver (at protein level). Mainly expressed in liver, small intestine, colon, adrenal gland and bladder.
數據庫鏈接：

HGNC: 17

OMIM: 600338

KEGG: hsa:13

STRING: 9606.ENSP00000232892

UniGene: Hs.506908