Recombinant Human 72 kDa type IV collagenase (MMP2)

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中文名稱：

Recombinant Human 72 kDa type IV collagenase (MMP2)
品名簡稱：

Recombinant Human MMP2 protein
貨號：

CSB-MP014666HU1
說明書：

MSDS Datasheet
規格：

￥828
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

MMP2
Uniprot No.：

P08253
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

Mammalian cell
分子量：

72.3 kDa
表達區域：

30-660aa
氨基酸序列

APSPIIKFPGDVAPKTDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQNTIETMRKPRCGNPDVANYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.; PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.; Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
基因功能參考文獻：
1. No significant associations were observed with either MMP-2(-1306) C/T or MMP-3(-1171) 5A/6A. PMID: 30117779
2. PROK1 or MMP-2 in the amniotic fluid do not have a role in the prediction of adverse pregnancy outcomes. PMID: 29405963
3. CDH17 has a role in altering MMP-2 expression via canonical NF-kappaB signalling in human gastric cancer PMID: 29783070
4. Study results revealed that CCR2 promotes epithelialtomesenchymal transition through MMP2 in liver cancer. PMID: 30132565
5. results of the study indicate that estradiol influence MMP-2 expression in the endometrium depends on the phase of menstrual cycle PMID: 30187906
6. Fewer TIMP-2, Hsp70 and TGF-beta1 immunoreactive cells in younger individuals and increased expression of Hsp70 in elderly individuals demonstrated the influence of aging in lung remodeling PMID: 29325453
7. Melatonin inhibits epithelialtomesenchymal transition in gastric cancer cells via attenuation of IL1beta/NFkappaB/MMP2/MMP9 signaling PMID: 30066836
8. TF-FVIIa/trypsin-mediated PAR2 activation leads to enhanced MMP-2 expression in human breast cancer cells contributing to tumor progression. PMID: 29870887
9. proteins such as MMP2 and MMP9 as well as P38 expression were also affected by the PKM2 expression changes. These results proved that PKM2 could be involved in the progression of bladder cancer by mitogen-activated protein kinases signaling pathway. PMID: 30249877
10. High MMP2 expression is associated with colorectal cancer cell migration, invasion and metastasis PMID: 30015978
11. We conducted a case-control genetic association study, adjusted for known IA risk factors (smoking and hypertension), in a UK Caucasian population of 1409 patients with intracranial aneurysms (IA), and 1290 matched controls, to determine the association of the rs243865 C > T functional MMP-2 gene SNP with IA (overall, and classified as ruptured and unruptured). PMID: 29334797
12. MMP2 rs243865 and MMP9 rs3918242 polymorphisms are significantly associated with the risk of RSA in Chinese population. PMID: 30290617
13. findings demonstrate an association between the MMP-2 C-735 T polymorphism with blood pressure and the risk of preeclampsia PMID: 29017408
14. The role of MMP 2 in asthma remains unclear and no study has been conducted till date, to determine the role of MMP-2 -735C/T gene polymorphism in asthma. This study does not disprove such association. PMID: 28051223
15. knockdown of TRIM31 reduced the expression of MMP2, MMP9 and phospho-Akt. PMID: 29864908
16. SB22055 could reduce the expression of BSP, OPN and MMP2 in prostate cancer cells. PMID: 29917166
17. Therefore, the A allele of MMP2 rs2287074 could be a protective factor for brick tea skeletal fluorosis. PMID: 28079131
18. MMP-2 genetic variants are an important mediator of stroke risk PMID: 30278505
19. HMGB1 promoted lung cancer invasion and metastasis by upregulating the expression and activity of MMP-2 in an NF-kappaB-dependent manner. PMID: 29850505
20. High glucose levels induce MMP-2 expression in Human cardiac fibroblasts , possibly by up-regulation of SGLT1. PMID: 29512713
21. we have discovered the novel N-72, and it was crucial for EGF-induced migration by targeting MMP2 in Human amnion mesenchymal stem cells (hAMSCs) PMID: 29734654
22. data indicated that MMP-2/MMP-9 was coordinately targeted by two non-coding RNAs, miR-296-3p and FOXCUT, which were decreased, and tumor-suppressing factors in choroidal malignant melanoma PMID: 29260433
23. Increased MMP-2 and MMP-9 activities may contribute to the pathogenesis and disease severity of cutaneous lupus erythematosus. PMID: 29349515
24. No association between the carriage of studied MMP2 polymorphisms and the predisposition to preeclampsia was found. PMID: 29670668
25. We propose that serum levels of MMP-2 and MMP-9 are not predictive on treatment response and survival in LARC patients. PMID: 29578156
26. that miR-338-5p has a function in promoting glioma cell invasion by targeting TSHZ3 suppression on MMP2 PMID: 28780604
27. In dilation cardiomyopathy, expression of MMP-2, MMP-9, and TIMP-1 and their ratios in autopsy material and in cultures was elevated by 1.5-9 times. PMID: 29504111
28. OSM [oncostatin M]might be involved in the invasiveness of extravillous trophoblasts under hypoxia conditions via increasing MMP-2 and MMP-9 enzymatic activities through STAT3 signaling. Increased MMP-9 activity by OSM seems to be more important in primary trophoblasts. PMID: 30091322
29. TIMP2 promotes tumor progression and miR2055p directly regulates TIMP2, thereby suppressing proMMP2 activation and inhibiting oral squamous cell carcinoma cell invasiveness. PMID: 29393341
30. Elevated MMP-2 levels were observed in blood of pancreatic cancer patients which correlated with its tissue expression. However, these levels did not associate with survival or any clinicopathological parameters of patients. PMID: 29355139
31. Increased urinary concentration of matrix metallopeptidase 2 (MMP-2) at 12 and 16 weeks of gestation predicted an increased risk of developing preeclampsia in the study population. PMID: 28055277
32. There was no difference found in MMP-2, MMP-9 or TLR-4 levels between non-thrombocytopenic and thrombocytopenic septic donors. PLA formation was increased in thrombocytopenic patients. PMID: 29734352
33. MMP2 rs243865 was the only single nucleotide polymorphism significantly associated with Floppy Mitral Valve/Mitral Valve Prolapse (FMV/MVP) as compared to the control.The frequency of certain MMP2 polymorphisms is higher in patients with the FMV/MVP syndrome and patients with FMV/MVP without the syndrome. PMID: 28750369
34. Platelets may contribute to joint degeneration in knee osteoarthritis by favouring the accumulation of MMP-2 in synovial fluid. PMID: 28981555
35. In pregnancy MMP2 gene expression was significantly higher in preterm than full term. Human papilloma viral load was found to be positively correlated to the rate of MMP2 expression and the gestational age was significantly related to the viral load and the rate of expression of MMP2 gene. Human pabilloma virus especially high-risk genotypes was correlated to spontaneous preterm labor in Egyptian females. PMID: 28264621
36. We conclude that S1P attenuates the invasion of C643 cells by activating S1P2 and the Rho-ROCK pathway, by decreasing calpain activity, and by decreasing the expression, secretion and activity of MMP2 and, to a lesser extent, MMP9. Our results thus unveil a novel function for the S1P2 receptor in attenuating thyroid cancer cell invasion. PMID: 29734379
37. High mmp2 expression is associated with ovarian cancer cell migration and invasion. PMID: 28677781
38. MMP-2 was expressed in high percentage of endometrial cancer and its expression may be associated closely with clinical stage, and tumor invasion and metastasis, indicating that MMP-2 overexpression may serve as a predictive factor for poor prognosis of endometrial cancer. PMID: 30024495
39. MMP2 single nucleotide polymorphisms association with lymphedema caused by Wuchereria bancrofti. PMID: 29122006
40. MMP-2 promoted and MMP-13 disrupted vasculogenic mimicry formation in large cell lung cancer by cleaving laminin-5 to influence EGFR signal activation. PMID: 28766880
41. Data suggest that environmental carcinogen PFOA (perfluorooctanoic acid) stimulates ovarian cancer cell migration, invasion, and MMP2/MMP9 expression by up-regulating ERK/NFkappaB signaling pathway. (MMP = matrix metallopeptidase; NFkappaB = nuclear factor kappa B) PMID: 29753068
42. Matrix-metalloproteinase-2 is implicated in the pathophysiological mechanism of stenosis development and has a predictive value for arteriovenous fistula failure in hemodialysis patients PMID: 28868755
43. Fli1 functioned as an oncogene in HCC carcinogenesis and it exerted its promoting metastatic effect primarily by modulating the matrix metalloproteinase (MMP)2 signaling pathway. PMID: 29138848
44. Rictor regulates the vasculogenic mimicry of melanoma and determines the patients' survival via the AKT-MMP2-MMP9 pathway. PMID: 28699701
45. Expression of BRM and MMP2 in the thoracic aortic aneurysm and aortic dissection is very high, indicating that BRM and MMP2 may play important roles in the occurrence and development of thoracic aortic aneurysm and aortic dissection. PMID: 28678310
46. at high endothelial shear stress, PI16 contributes to inhibition of MMP2 protease activity, protection that can be reversed during inflammation PMID: 27996045
47. MMP-2 serum level and circulating tumor cells show the potential to predict CNS metastases and overall survival in breast cancer patients; CTCs and MMP-9 serum level could be a promising therapy response marker in castration resistant prostate cancer patients PMID: 28529342
48. RhoGDIbeta overexpression led to downregulation of miR-200c, whereas miR-200c was able directly to target 3'-UTR of jnk2mRNA and attenuated JNK2 protein translation, which resulted in attenuation of Sp1mRNA and protein expression in turn, inhibiting Sp1-dependent MMP-2 transcription. PMID: 28846829
49. both HBEGF upregulation and apoptosis were rescued by exogenous MMP2 PMID: 28731464
50. MMP-2 (and MMP-1 and MMP-3) are independently associated with markers of arterial stiffening in patients with type 1 diabetes. PMID: 29070037
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相關疾病：

Multicentric osteolysis, nodulosis, and arthropathy (MONA)
亞細胞定位：

[Isoform 1]: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.; [Isoform 2]: Cytoplasm. Mitochondrion.
蛋白家族：

Peptidase M10A family
組織特異性：

Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
數據庫鏈接：

HGNC: 7166

OMIM: 120360

KEGG: hsa:4313

STRING: 9606.ENSP00000219070

UniGene: Hs.513617