Recombinant Human 14-3-3 protein zeta/delta (YWHAZ)

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中文名稱：

Recombinant Human 14-3-3 protein zeta/delta (YWHAZ)
品名簡稱：

Recombinant Human YWHAZ protein
貨號：

CSB-EP026293HU
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

YWHAZ
Uniprot No.：

P63104
別名：

Protein kinase C inhibitor protein 1
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

34.7 kDa
表達區域：

1-245aa
氨基酸序列

MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, regulates spine maturation through the modulation of ARHGEF7 activity.
基因功能參考文獻：
1. Amount of 14-3-3 proteins is decreased in pineal gland, blood platelets and ileum of patients with ASD. PMID: 28522826
2. These results imply that disorder in the N-terminal helices of 14-3-3 zeta is a consequence of the dimer-monomer dynamics and may play a role in conferring chaperone function to 14-3-3 zeta protein. PMID: 29109150
3. Knockdown of YWHAZ inhibited cell cycle progression, migration, and the expression of stem cell markers and tumorigenicity was suppressed in tumor-bearing BALB/c nude mice. The expression of YWHAZ was directly down-regulated by miR-30e in resistant ovarian cancer cells. PMID: 30134224
4. our data suggest miR-204 and 14-3-3zeta as potential therapeutic targets in osteosarcoma PMID: 29441884
5. evidence is lacking to conclude that 14-3-3zeta is a useful marker of tamoxifen resistance. PMID: 28643021
6. TRIM21 positively regulated osteosarcoma cell proliferation. Overexpression of TRIM21 enhanced osteosarcoma cell tolerance toward various stresses. YWHAZ protein was identified as a novel interacting partner of TRIM21 and its expression levels were negatively regulated by TRIM21. PMID: 29673441
7. several disordered regions of PI4KB become protected from proteolytical degradation upon 14-3-3 binding. PMID: 28864297
8. Ectopic expression of miR-451 could inhibit the cell migration and invasion, promoted apoptosis, induced cell-cycle arrest Furthermore, tyrosine3-monooxygenase/tryptophan5-monooxygenase activation protein zeta (YWHAZ) was identified as a direct target of miR-451 PMID: 28981108
9. Serum autoantibodies to YWHAZ are produced at substantially greater levels in gastric cancer patients as compared to controls. PMID: 28944820
10. Dimerization of 14-3-3 zeta (14-3-3zeta) dimer was disrupted by a double mutant (L12E, M78K). PMID: 29203375
11. Results identified YWHAZ as the direct target of miR-613 in hepatocellular carcinoma (HCC). Its overexpression reverses the tumor suppressing role of miR-613 in HCC cells. PMID: 29551505
12. 14-3-3zetaoverexpression might be a potential prognostic biomarker for ovarian cancer. PMID: 29214776
13. In AML patients, low level of miR-451 is negatively correlated with high levels of c-Myc and YWHAZ, while c-Myc level is positively related to YWHAZ expression. These results suggested that c-Myc dash, verticalmiR-451 dash, verticalYWHAZ/AKT cascade might play a crucial role during leukemogenesis, and reintroduction of miR-451 could be as a potential strategy for AML therapy. PMID: 27764807
14. miR-22 exhibits tumor-suppressive effects in hepatocellular carcinoma cells by regulating YWHAZ/AKT/FOXO3a signaling. PMID: 27811373
15. our data demonstrate that overexpression of 14-3-3zeta in early stage pre-cancerous breast epithelial cells may trigger an elevated glycolysis and transcriptionally up-regulating LDHA, thereby contributes to human breast cancer initiation. PMID: 27150057
16. 14-3-3zeta can bind to the FOXO3a transcription factor to promote the export of the complex to the cytoplasm, leading to enhanced proliferation and migration of tongue cancer cells. PMID: 27080223
17. Structure of the complex of phosphorylated liver kinase B1 and 14-3-3zeta has been reported. PMID: 28368277
18. These results suggest that the hypoxia/14-3-3zeta/HIF-1alpha pathway plays an important role in portal vein tumor thrombus formation and hepatocellular carcinoma metastasis PMID: 26910835
19. 14-3-3zeta recruited YAP and p-LATS to form a complex under high cells density status and 14-3-3zeta other than YAP or phospho-LATS was the key regulatory molecule of this complex. PMID: 27334574
20. This study shows that human procaspase-2 interaction with 14-3-3 zeta is governed by phosphorylation at both S139 and S164. PMID: 28943433
21. The results highlight a new role of TSC2 in protecting glioblastoma against photodynamic therapy-induced cell death, and TSC2 and YWHAZ as new RIP3 partners. PMID: 27984090
22. These results suggest that 14-3-3-zeta is involved in the TLR3-TICAM-1 pathway in promoting multimerization of TICAM-1 for the formation of a TICAM-1 signalosome. PMID: 27058640
23. The data indicate that microtubule-bound tau is resistant to 14-3-3zeta-induced tau aggregation and suggest that tau phosphorylation promotes tau aggregation in the brain by detaching tau from microtubules and thus making it accessible to 14-3-3zeta. PMID: 27548710
24. Structural interface between LRRK2 and 14-3-3 delta protein has been presented. PMID: 28202711
25. 14-3-3zeta-mediated invasion of cancer cells was found to upregulate Snail through the activation of atypical protein kinase C (aPKC). PMID: 27554601
26. results have identified a novel mechanism by which 14-3-3sigma maintains the epithelial phenotype by inhibiting Epithelial to Mesenchymal Transition and suggest that this property of 14-3-3sigma might contribute to its function as a tumor suppressor gene. PMID: 27261462
27. 14-3-3zeta regulates HIF-1alpha production in hepatocellular carcinoma cells by directly binding to HIF-1alpha and via PI3K/Akt/NF-small ka, CyrillicB signal transduction pathway. PMID: 26884855
28. Results indicate that HuR induces 14-3-3zeta translation via interaction with its 3' UTR and that 14-3-3zeta is necessary for stimulation of intestinal epithelial cell migration after wounding. PMID: 27401462
29. this study suggests that the down-regulation of 14-3-3 zeta leads to the inhibition of TGFb1- induced contraction by decreasing the expression of total RhoA in TM cells. PMID: 26906158
30. loss of expression or even the down-regulation of c-abl, but not WYHAZ, is a fundamental event that leads to genesis and progression of tumors PMID: 26429164
31. This study provides the molecular basis for C-Raf C-terminal-derived phosphopeptide interaction with 14-3-3zeta protein and gives structural insights responsible for phosphorylation-mediated protein binding. PMID: 26295714
32. 14-3-3z may play an important role in signaling pathway in breast cancer. Also, a high 14-3-3z expression could positively regulate growth factor receptors and protein kinase pathways PMID: 25861752
33. Studies show that 14-3-3zeta is overexpressed in oral squamous cell carcinoma and provide evidence that may regulate tumor inflammation and immune response through Stat3 signaling. PMID: 25556369
34. Activation of PCTAIRE-1 is mediated through interaction with the phosphorylated form of cyclin Y in complex with 14-3-3. PMID: 26205494
35. C-terminal domain of Pdc interacts with the outside surface of the 14-3-3 dimer. PMID: 25971962
36. Our findings indicate that YWHAZ could serve as a promising prognostic biomarker in localized PCa to predict poor prognosis PMID: 25156059
37. studyconfirmed the interaction of Ser9-phosphorylated GSK3beta with 14-3-3zeta; Ser9-phosphorylation of GSK3beta promoted by 14-3-3zeta is critical for the activation of NF-kappaB pathway PMID: 25138042
38. A detailed analysis of the interaction between singly or doubly phosphorylated human tyrosine hydroxylase isoform 1(1-50) peptides and 14-3-3zeta PMID: 25418103
39. BIS targeting induces cellular senescence through the regulation of 14-3-3 zeta/STAT3/SKP2/p27 in glioblastoma cells. PMID: 25412315
40. Aberrant upregulation of 14-3-3sigma and EZH2 expression serves as an inferior prognostic biomarker for hepatocellular carcinoma. PMID: 25226601
41. The 14-3-3zeta-driven contextual changes of Smad partners from p53 to Gli2 may serve as biomarkers and therapeutic targets of TGF-b-mediated cancer progression. PMID: 25670079
42. Among the genes found disrupted in this study, there is evidence suggesting that YWHAZ and also the X-linked DRP2 may be considered as novel autism candidate genes. PMID: 23999528
43. Data found that the interaction between 14-3-3 zeta and Atg9A is mediated by phosphorylation at Ser761. PMID: 25266655
44. miR-375-mediated regulation of 14-3-3zeta contributes to decrease telomerase activity by altering nuclear translocation of TERT. PMID: 24708873
45. 14-3-3zeta regulates nuclear trafficking of PP1alpha in mammalian cells PMID: 24956593
46. By preventing the inactivation of cofilin, metabolic stress-induced degradation of 14-3-3zeta promotes the conversion of blood monocytes into a hypermigratory, proatherogenic phenotype. PMID: 24812321
47. Compared to HL-60 cells, multidrug-resistant HL-60/VCR cells had increased 14-3-3zeta mRNA and protein expression.Silencing of 14-3-3zeta increased the sensitivity of both sensitive and resistant HL-60 cells to TPT-induced apoptosis. PMID: 24603438
48. 14-3-3zeta causes synaptic loss by destabilizing microtubules, leading to proteosomal degradation of synaptophysin in the neurons of patients suffering from Alzheimer's disease. PMID: 24367683
49. Data suggest that the combined expression of 14-3-3zeta and Hsp27 may be a biomarker for predicting survival in patients with NSCLC, and this combination may have potential as a therapeutic target for NSCLC. PMID: 24804299
50. Somatic copy number alterations by whole-exome sequencing implicates YWHAZ and PTK2 in castration-resistant prostate cancer. PMID: 24114522
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亞細胞定位：

Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.
蛋白家族：

14-3-3 family
數據庫鏈接：

HGNC: 12855

OMIM: 601288

KEGG: hsa:7534

STRING: 9606.ENSP00000309503

UniGene: Hs.492407