Recombinant Escherichia coli RNA-binding protein Hfq (hfq)

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Stock}

貨號：

CSB-EP363917ENV
說明書：

MSDS Datasheet
規格：
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

hfq
Uniprot No.：

P0A6X3
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

18.5 kDa
表達區域：

2-102aa
氨基酸序列

AKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVILLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Involved in the regulation of stress responses mediated by the sigma factors RpoS, sigma-E and sigma-32. Binds with high specificity to tRNAs. Binds sRNA antitoxin RalA. In vitro, stimulates synthesis of long tails by poly(A) polymerase I. Required for RNA phage Qbeta replication. Seems to play a role in persister cell formation; upon overexpression decreases persister cell formation while deletion increases persister formation.
基因功能參考文獻：
1. By interacting with the mutS leader, Hfq serves as a critical switch that modulates bacteria from high-fidelity DNA replication to stress-induced mutagenesis PMID: 28794186
2. Here, de novo Rosetta modeling and competitive binding experiments show that the acidic tip of the Escherichia coli Hfq CTD transiently binds the basic Sm core residues necessary for RNA annealing. PMID: 28826489
3. Compaction and condensation of DNA mediated by the C-terminal domain of Hfq has been reported. PMID: 28521053
4. Global mapping of small RNA-Hfq target interactions have been described in the bacterial genome. PMID: 27588604
5. We describe a refined system - consisting solely of purified Hfq, polynucleotide phosporylase (PNP) and ADP - that allows reconstitution in vitro of characteristic 'SDS-insensitive' Hfq6-Hfq6 assemblies observed in experiments with whole-cell extracts obtained from exponentially-growing cells. We also optimized conditions for the extraction of intact native dodecameric Hfq complexes. PMID: 28366634
6. The 3' terminal oligo(U) stretch and the base-pairing region enable small RNA to interact with Hfq. PMID: 26531825
7. Hfq has at least two distinct ways in which it interacts with sRNAs; these different binding properties have strong effects on the stability of the sRNA in vivo and the sequence requirements of regulated mRNAs. PMID: 26373314
8. The authors show that an interaction between the distal surface of Hfq and the ribosome-binding site of IS10 transposase mRNA (RNA-IN) is required for repressing translation initiation of the transposase. PMID: 25649688
9. This study demonstrated that random-order binding of Hfq to sRNA and mRNA in each pair can cause high Hfq concentrations to suppress rather than promote sRNA activity in Escherichia coli. PMID: 26261213
10. Study shows that Hfq does not affect the transcriptional readthrough event itself; also find that the readthrough products no longer bind to Hfq in vivo. PMID: 26106215
11. these results suggest that Hfq could function as an anchor/coupling factor responsible for de-solubilization of RNA and its tethering to the degradosome complex. PMID: 25896386
12. The study verified that the A-rich linker region of OxyS sRNA binds Hfq at its distal side. PMID: 25670676
13. Data show that the mRNA wraps entirely around the host Ffactor 1 protein Hfq protein, specifically contacting both surfaces. PMID: 25404287
14. The data indicated that the simultaneous binding to the opposite faces of Hfq was enabled by separate adenosine-rich and uridine-rich sequences in the long, single-stranded region of ChiX. PMID: 25582129
15. Identification of RNA binding sites of Hfq. PMID: 24288369
16. Hfq acts as the repressor of cirA mRNA translation in the absence of sRNA. PMID: 24065131
17. Based on the results presented here, we suggest that Hfq could be involved in regulating RNA pairing in other antisense systems, including systems encoded by other transposable elements. PMID: 23510801
18. Thus, Hfq is intrinsically essential to pathogenic Escherichia coli for survival and virulence exerted in the Caenorhabditis elegans host. PMID: 22713744
19. The authors show that Hfq is most active in RNA annealing when the hexamer is present. PMID: 22326348
20. sRNAs compete with each other for Hfq binding. PMID: 22040174
21. expression of hfq was repressed by the CRP-cAMP complex. PMID: 21840983
22. the purification, crystallization and structural data of the full 102-amino-acid protein are presented. These data revealed that the presence of the C-terminus changes the crystal packing of E. coli Hfq PMID: 21543856
23. Results revealed that the C-terminus of Hfq is intrinsically disordered and flexible, which appears to contribute to the interactions of Hfq with RNA substrates. PMID: 21330354
24. role of Hfq in LF82 virulence was independent of its function in regulating RpoS and RpoE activity PMID: 21298102
25. Hfq negatively regulate Tn10/IS10 transposition by promoting anti-sense inhibition of RNA-IN10 translation. PMID: 20815820
26. Hfq mediates the binding of sRNAs to their target mRNAs; without Hfq, most sRNAs cannot efficiently regulate target mRNA expression. PMID: 21189298
27. examined the ability of Hfq derivatives, consisting of the conserved core and short C-terminal extensions, to support the regulation of rpoS expression and riboregulation by various well-characterized small regulatory RNAs PMID: 20888338
28. LEE regulator (GrlA) is critically involved in the regulating the presence of L0045. PMID: 20587027
29. show that both Hfq and this Hfq binding site are required for RprA or ArcZ to act in vivo and to form a stable complex with rpoS mRNA in vitro PMID: 20457943
30. The alteration of rpsO, rpsT and rpsB-tsf transcript levels observed under conditions of Hfq deficiency is not caused by the post-transcriptional events. PMID: 20167073
31. Hfq has two independent RNA-binding surfaces; Hfq can simultaneously bind a ncRNA and its mRNA target to facilitate the strand displacement reaction required for Hfq-dependent translational regulation PMID: 15531892
32. Hfq synthesis is autoregulated at the translational level. PMID: 15872186
33. E.coli Hfq polymerizes into well-ordered fibres whose morphologies closely resemble those found for Sm-like archaeal proteins PMID: 16337963
34. findings indicate that Hfq regulates traM and traJ transcript stability by a mechanism separate from FinOP-mediated repression PMID: 16352828
35. Study shows that SgrS RNA forms a stable duplex with the phosphoenolpyruvate-glucose phosphotransferase mRNA, and that Hfq markedly facilitates the rate of duplex formation. PMID: 16859494
36. Hfq may play a general role in stress response regulation in E. coli. PMID: 17158661
37. Hfq modulates the interaction between a small non-coding RNA, DsrA, and its regulatory target mRNA, rpoS. PMID: 17259214
38. structure and function of Hfq [review] PMID: 17395525
39. The increase of the CCA-addition in the presence of Hfq suggests that this protein acts as a stimulating factor not only for PAP, but also for the CCA-adding enzyme. PMID: 17949481
40. The C-terminal extension of E. coli Hfq constitutes a hitherto unrecognized RNA interaction surface with specificity for mRNAs. PMID: 18000007
41. tRNA binding and reduced fidelity are linked by a role for Hfq in tRNA modification. PMID: 18230766
42. Hfq negatively affects LEE expression in exponential phase by regulating the expression of the LEE-encoded regulators GrlA and GrlR at the post-transcriptional level by destabilizing grlRA mRNA. PMID: 19570135
43. HFq protein is involved in the regulation of bacterial cytoskeleton and cell division proteins. PMID: 19571680
44. Study showed a novel property of Escherichia coli Hfq protein: it possibly influenced extracellular indole levels. PMID: 19657572
45. the crystal structure of Escherichia coli Hfq bound to the poly(A) RNA, A(15) is reported. PMID: 19889981
46. Hfq binds with high specificity and nanomolar affinity to both D-stem and T-stem of tRNAs using its proximal surface. PMID: 18230766
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蛋白家族：

Hfq family
數據庫鏈接：

KEGG: ecj:JW4130

STRING: 316385.ECDH10B_4367