Recombinant Escherichia coli Phage shock protein A (pspA)

中文名稱：

大腸桿菌pspA重組蛋白
貨號：

CSB-YP360290ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌pspA重組蛋白
貨號：

CSB-EP360290ENV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌pspA重組蛋白
貨號：

CSB-EP360290ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌pspA重組蛋白
貨號：

CSB-BP360290ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌pspA重組蛋白
貨號：

CSB-MP360290ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

pspA
Uniprot No.：

P0AFM6
別名：

pspA; b1304; JW1297; Phage shock protein A
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-222
氨基酸序列

GIFSRFADI VNANINALLE KAEDPQKLVR LMIQEMEDTL VEVRSTSARA LAEKKQLTRR IEQASAREVE WQEKAELALL KEREDLARAA LIEKQKLTDL IKSLEHEVTL VDDTLARMKK EIGELENKLS ETRARQQALM LRHQAANSSR DVRRQLDSGK LDEAMARFES FERRIDQMEA EAESHSFGKQ KSLDDQFAEL KADDAISEQL AQLKAKMKQD NQ
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation of the psp-specific transcriptional activator PspF. Is also required for membrane integrity, efficient translocation and maintenance of the proton motive force.
基因功能參考文獻：
1. Structure and function of Escherichia coli PspA and Synechocystis Vipp1 N-terminal peptides in the membrane stress sensing and mitigation have been described. PMID: 27806910
2. Escherichia coli PspA has an enhanced sensitivity for curvature elastic (SCE) stress and a higher affinity for the membrane than Synechocystis Vipp1. PMID: 26330516
3. The discrete spatial distribution and dynamics of PspA effector in lateral membrane regions depend on MreB and RodZ. PMID: 25118250
4. Multiple signals are needed to induce the Psp response. PMID: 21259006
5. These results indicate that oligomers of PspA bind to membrane phospholipids and suppress proton leakage. PMID: 17725563
6. PspA can form hollow spherical or prolate spheroidal particles of about 30-40nm diameter with a scaffold-like arrangement of protein subunits at the surface. PMID: 18789328
7. PspA and PspG are localized to the cell poles and traffic between both poles. PMID: 19555453
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亞細胞定位：

Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes at both cell poles and along the length of the cell.
蛋白家族：

PspA/IM30 family
數據庫鏈接：

KEGG: ecj:JW1297

STRING: 316385.ECDH10B_1421