Recombinant Escherichia coli Membrane protein insertase YidC (yidC), partial

中文名稱：

大腸桿菌yidC重組蛋白
貨號(hào)：

CSB-YP340722ENV1
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

大腸桿菌yidC重組蛋白
貨號(hào)：

CSB-EP340722ENV1
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

大腸桿菌yidC重組蛋白
貨號(hào)：

CSB-EP340722ENV1-B
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌yidC重組蛋白
貨號(hào)：

CSB-BP340722ENV1
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

大腸桿菌yidC重組蛋白
貨號(hào)：

CSB-MP340722ENV1
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

yidC
Uniprot No.：

P25714
別名：

yidC; b3705; JW3683; Membrane protein insertase YidC; Foldase YidC; Inner membrane protein YidC; Membrane integrase YidC; Oxa1Ec
種屬：

Escherichia coli (strain K12)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Inner membrane protein required for the insertion and/or proper folding and/or complex formation of integral inner membrane proteins. Involved in integration of membrane proteins that insert dependently and independently of the Sec translocase complex, as well as at least 2 lipoproteins. Its own insertion requires SRP and is Sec translocase-dependent. Essential for the integration of Sec-dependent subunit a of the F(0)ATP synthase, FtsQ and SecE proteins and for Sec-independent subunit c of the F(0)ATP synthase, M13 phage procoat and the N-terminus of leader peptidase Lep. Probably interacts directly with Sec-independent substrates. Sec-dependent protein FtsQ interacts first with SecY then subsequently with YidC. Sec-dependent LacY and MalF require YidC to acquire tertiary structure and stability, a chaperone-like function, but not for membrane insertion. Stable maltose transport copmplex formation (MalFGK(2)) also requires YidC. Partially complements a Streptococcus mutans yidC2 disruption mutant.
基因功能參考文獻(xiàn)：
1. We suggest that EH1 serves as a mechanical lever, facilitating a coordinated movement of YidC TMDs to trigger the release of nascent chains into the membrane. PMID: 27974208
2. The YidC prevents LacY from misfolding by stabilizing the unfolded state from which LacY inserts structural segments stepwise into the membrane until folding is completed. During stepwise insertion, YidC and the membrane together stabilize the transient folds. PMID: 27595331
3. Deletions in loop C1 affected the activity of YidC but did not influence ribosome binding or substrate insertion, whereas loop C2 was involved in ribosome binding. Combining the deletion with removal of the C terminus abolished YidC-mediated insertion. PMID: 26023232
4. These findings extend the function of YidC as a foldase for membrane protein (complexes) to periplasmic domains of membrane proteins. PMID: 25666136
5. Data indicate that the conserved positively charged residue was not required for the E. coli YidC or the A. thaliana Alb3 to functionally complement the E. coli YidC depletion strain or to promote insertion of YidC-dependent membrane proteins. PMID: 25359772
6. Conserved proline residues on the cytoplasmic side of conserved core transmembrane segments contribute to flexibility and structure of the protein YidC. PMID: 24801559
7. The YidC protein is critical for the insertion of a subset of proteins into membranes. PMID: 22108384
8. The data show the broad physiological contribution of YidC to the bacterial cell and the considerable ramification to the cell when it is depleted. PMID: 20061485
9. the Sec-independent function of YidC is conserved and essential for cell growth PMID: 15671040
10. Required for membrane insertion of F1F0 ATP synthase. PMID: 16880204
11. Both alleles impart a cold-sensitive phenotype and result from point mutations localized to the third transmembrane (TM3) segment of YidC, indicating that this region is crucial for YidC function. PMID: 17933892
12. they define the features of a membrane protein transmembrane segment that induce lateral release from the YidC and Sec translocases into the lipid bilayer in bacteria PMID: 18052199
13. The region corresponding to the C-terminal alpha-helix was previously shown to be important for the protein insertase function of YidC and is conserved in other YidC-like proteins. PMID: 18093969
14. YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex PMID: 18456666
15. Expression and purification of the recombinant membrane protein YidC PMID: 18586516
16. specific residues of the third YidC transmembrane segment alpha-helix is oriented toward the transmembrane domains of nascent inner membrane proteins that, in contrast, appear quite flexibly positioned at this stage in biogenesis PMID: 18840604
17. A detailed disulfide-mapping study revealed that one face of TM3 of YidC makes contact with the Pf3 protein. PMID: 18996118
18. Results suggest that dimers of the YidC and Oxa1 proteins form insertion pores and share a common overall architecture with the SecY monomer. PMID: 19450532
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亞細(xì)胞定位：

Cell inner membrane; Multi-pass membrane protein. Note=Predominantly localized at cell poles at all stages of cell growth.
蛋白家族：

OXA1/ALB3/YidC family, Type 1 subfamily
數(shù)據(jù)庫(kù)鏈接：

KEGG: ecj:JW3683

STRING: 316385.ECDH10B_3892