Recombinant Escherichia coli Lactose operon repressor (lacI) (Q360QC)

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中文名稱：

大腸桿菌lacI重組蛋白
貨號：

CSB-EP365825ENV(M)
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

lacI
Uniprot No.：

P03023
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length
來源：

E.coli
分子量：

44.7 kDa
表達區域：

1-360aa(Q360QC)
氨基酸序列

MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Repressor of the lactose operon. Binds allolactose as an inducer.
基因功能參考文獻：
1. Effect of helix length on the stability of the Lac repressor antiparallel coiled coil. PMID: 25969365
2. LacI autoregulation balances two opposing states, one that allows quicker response to smaller pulses of external lactose, and the other that minimizes production costs in the absence of lactose. PMID: 23658223
3. Data indicate that in the absence of its inducer, LacI binds to primary operator lac O1 near the transcription start site of the lac operon, and represses its transcription. PMID: 22594825
4. findings show the lac repressor slides 45 +/- 10 base pairs on chromosomal DNA and that sliding can be obstructed by other DNA-bound proteins near the operator; the repressor frequently slides over its natural lacO(1) operator several times before binding PMID: 22723426
5. The role of lactose repressor residues asparagine-125 and aspartate-149 illustrate how readily the ligand binding and allosteric response function of a regulatory bacterial protein can be altered, generating diminished operator binding. PMID: 21928765
6. Using the extensive LacI family of transcriptional factors, identified a set of specificity correlations--determined by two AAs of the TFs and two NTs in the binding sites- and that act as a relatively consistent recognition code. PMID: 21085639
7. solution structure and dynamics of the complex of a dimeric lac repressor DNA binding domain with nonspecific DNA PMID: 15256668
8. The Lac repressor artificially attached to the Escherichia coli cytoplasmic membrane is able to perfectly represses transcription initiated at the tac operator-promoter present on a plasmid and even in the chromosome. PMID: 15867195
9. Linear DNA segments of short-to-medium chain length (50-180 bp) give rise to loops with the extended form of LacR and loops formed within negatively supercoiled plasmids induce the V-shaped structure. PMID: 16785444
10. The mutational spectrum of the genomic lacI gene induced by low-energy nitrogen ion irradiation in wild type Escherichia coli strain W3110 were compared with the spontaneous and the vacuum controls. PMID: 17049362
11. In single molecule experiments, both specific bending and non-specific compaction by integration host factor alter LacI-mediated looping of DNA PMID: 17543441
12. The altered hydrogen bonding can account in part for the different structural conformations of the repressor, and is vital for the allosteric transition PMID: 17543986
13. The structure of lactose repressor (LacI) protein confirms previously reported structures on the monomer level, however, the tetramer is much more densely packed. PMID: 19004002
14. irradiation induces the change of the oligomerization state of LacI from tetramer to dimer. PMID: 19520056
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數據庫鏈接：

KEGG: ecj:JW0336

STRING: 316385.ECDH10B_1357