Recombinant Escherichia coli Esterase ybfF (ybfF)

中文名稱：

大腸桿菌ybfF重組蛋白
貨號：

CSB-YP305118ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌ybfF重組蛋白
貨號：

CSB-EP305118ENV-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌ybfF重組蛋白
貨號：

CSB-BP305118ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌ybfF重組蛋白
貨號：

CSB-MP305118ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

ybfF
Uniprot No.：

P75736
別名：

ybfF; b0686; JW0673; Esterase YbfF; EC 3.1.-.-
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-254
氨基酸序列

MKLNIRAQTA QNQHNNSPIV LVHGLFGSLD NLGVLARDLV NDHNIIQVDM RNHGLSPRDP VMNYPAMAQD LVDTLDAQQI DKATFIGHSM GGKAVMALTA LASDRIDKLV AIDIAPVDYH VRRHDEIFAA INAVSESDAQ TRQQAAAIMR QHLNEEGVIQ FLLKSFVDGE WRFNVPVLWD QYPHIVGWEK IPAWDHPALF IPGGNSPYVS EQYRDDLLAQ FPQARAHVIA GAGHWVHAEK PDAVLRAIRR YLND
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate.
基因功能參考文獻(xiàn)：
1. This approach led to the identification of esterase YbfF as the major Escherichia coli enzyme responsible for the hydrolytic activity toward 1,2-O-isopropylideneglycerol esters. PMID: 21764964
2. With two Ec_ybfF molecules in the asymmetric unit, the crystal volume per unit protein weight is 2.17 A(3) Da(-1), corresponding to a solvent content of 42% PMID: 18084091
3. The high-resolution structure of ybfF shows that the inserted helical bundle structure is critical for creating a large inner space, thereby providing the broad substrate spectrum toward large biomolecules. PMID: 18215690
蛋白家族：

DmpD/TodF/XylF esterase family
數(shù)據(jù)庫鏈接：

KEGG: ecj:JW0673

STRING: 316385.ECDH10B_0751