Recombinant Escherichia coli Endonuclease 4 (nfo)

中文名稱：

大腸桿菌nfo重組蛋白
貨號(hào)：

CSB-YP363838ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌nfo重組蛋白
貨號(hào)：

CSB-EP363838ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌nfo重組蛋白
貨號(hào)：

CSB-EP363838ENV-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌nfo重組蛋白
貨號(hào)：

CSB-BP363838ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌nfo重組蛋白
貨號(hào)：

CSB-MP363838ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

nfo
Uniprot No.：

P0A6C1
別名：

nfo; b2159; JW2146Endonuclease 4; EC 3.1.21.2; Endodeoxyribonuclease IV; Endonuclease IV
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-285
氨基酸序列

MKYIGAHVSA AGGLANAAIR AAEIDATAFA LFTKNQRQWR AAPLTTQTID EFKAACEKYH YTSAQILPHD SYLINLGHPV TEALEKSRDA FIDEMQRCEQ LGLSLLNFHP GSHLMQISEE DCLARIAESI NIALDKTQGV TAVIENTAGQ GSNLGFKFEH LAAIIDGVED KSRVGVCIDT CHAFAAGYDL RTPAECEKTF ADFARTVGFK YLRGMHLNDA KSTFGSRVDR HHSLGEGNIG HDAFRWIMQD DRFDGIPLIL ETINPDIWAE EIAWLKAQQT EKAVA
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.
基因功能參考文獻(xiàn)：
1. Novel Nfo substrates alpha-2'-deoxyadenosine and alpha-thymidine play an important role in counteracting oxidative DNA base damage. PMID: 15568813
2. analysis of the three-metal-ion catalytic mechanism of the DNA repair enzyme endonuclease IV PMID: 17242363
3. Biochemical, mutational & crystallographic characterization reveal a 3-metal ion mechanism for damage binding and incision; DNA-free and DNA-substrate complex structures capture substrate stabilization by Arg37 and show a distorted Zn3 ligand arrangement. PMID: 18408731
蛋白家族：

AP endonuclease 2 family
數(shù)據(jù)庫鏈接：

KEGG: ecj:JW2146

STRING: 316385.ECDH10B_2316