Recombinant Drosophila melanogaster Epidermal growth factor receptor (Egfr), partial

中文名稱：

黑腹果蠅Egfr重組蛋白
貨號：

CSB-YP007479DLU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

黑腹果蠅Egfr重組蛋白
貨號：

CSB-EP007479DLU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

黑腹果蠅Egfr重組蛋白
貨號：

CSB-EP007479DLU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

黑腹果蠅Egfr重組蛋白
貨號：

CSB-BP007479DLU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

黑腹果蠅Egfr重組蛋白
貨號：

CSB-MP007479DLU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Egfr
Uniprot No.：

P04412
別名：

Egfr; c-erbB; DER; top; CG10079; Epidermal growth factor receptor; Egfr; EC 2.7.10.1; Drosophila relative of ERBB; Gurken receptor; Protein torpedo
種屬：

Drosophila melanogaster (Fruit fly)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Receptor tyrosine kinase, binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include spitz, gurken, vein and giant-lens. Transduces the signal through the ras-raf-MAPK pathway. Critical for the proliferation of imaginal tissues, and for the determination of both the antero-posterior and dorso-ventral polarities of the oocyte. In the embryo, plays a role in the establishment of ventral cell fates, maintenance of amnioserosa and ventral neuroectodermal cells, germ band retraction, cell fate specification in the central nervous system, and production and repair of the cuticle. During dorsal closure (DC) functions with the dpp- and ACK-signaling pathways to regulate expression of the myosin zip in the embryonic epidermis and amnioserosa (AS), and thus coordinate the progression of epidermal cell shape changes required for correct DC. In the embryonic epidermis, functions by negatively regulating dpp and consequently the dpp-dependent expression of the myosin zip. In the AS, negatively regulates the production/ and or secretion of a diffusible signal which, is produced by the ACK-signaling pathway, and acts in the AS and epidermal cells to promote zip expression. Also required in the AS to inhibit or delay apoptosis, and consequently slow the rate of DC. Therefore functions at multiple levels to negatively regulate morphogenesis during DC, suggesting that it acts as a general brake mechanism for adjusting the rate of dorsal closure to ensure that closure proceeds smoothly and without loss of epidermal integrity. During oogenesis, one of two tyrosine kinase chemoattractant receptors (Egfr and Pvr), that function in the border cells (BC) to detect guidance cues from the oocyte and transduce this information to the guidance pathway that regulate the collective migration of the BC cluster through the nurse cells to the oocyte.
基因功能參考文獻：
1. stress-dependent EGFR/MAPK promotes gut regeneration via a novel mechanism that operates independently of Insulin/Pi3K/TOR signaling. PMID: 28485389
2. Data show that EGFR controls the proper formation of brain neuroblasts by regulating the number, survival and proneural gene expression of neuroectodermal progenitor cells which suggest that EGFR signalling is crucially important for patterning and early neurogenesis of the brain. PMID: 27974623
3. The activity of Gro is antagonized by EGFR signaling, which inhibits Gro-dependent repression via p-ERK mediated phosphorylation. PMID: 27836963
4. we find that EGFR regulates the apical determinant Crb and the extracellular matrix regulator Serp, two factors previously known to control tube length. EGFR regulates the organisation of endosomes in which Crb and Serp proteins are loaded PMID: 28678789
5. Avermectin directly interacts with EGFR and leads to the activation of the EGFR/AKT/ERK pathway. PMID: 27249340
6. The dorsoventral patterning and EGFR signaling genes play essential roles in correct identity determination and differentiation of lateral glia in the Drosophila nervous system. PMID: 26318336
7. Our findings provide in vivo evidence for the role of adult neurons in the maintenance of glia and a novel role for EGFR signaling in the autophagic flux. PMID: 25909451
8. Gro inhibits rho expression in undifferentiated cells and represses the expression of both ato and rho in non-R8 precursors during initiation of photoreceptor differentiation in an E(spl)-dependent manner. PMID: 26417727
9. strategy for producing ordered square cell packing configurations in epithelia and reveal a molecular mechanism by which organized tissue structure is generated through spatiotemporally regulated responses to EGF receptor activation. PMID: 26506305
10. the findings indicate that Vps4 can promote EGFR activity through an endocytosis-independent mechanism. PMID: 25790850
11. Drosophila compound eye development is a good model for studying the Egfr signaling pathway. PMID: 25707069
12. Mechanistically, miR-998 operates by repressing dCbl, a negative regulator of EGFR signaling. Significantly, dCbl is a critical target of miR-998 since dCbl phenocopies the effects of miR-998 on dE2f1-dependent apoptosis in rbf mutants PMID: 25058496
13. FGFR signaling most likely affects ethanol-induced behavior through a different mechanism, possibly through acute action in adult neurons. PMID: 24498174
14. Epidermal growth factor receptor (Egfr) signaling in somatic stromal intermingled cells (ICs), activated by its ligand produced in germ cells, controls the size of the primoridal germ cell pool at the onset of gametogenesis. PMID: 23376160
15. Terminal differentiation is promoted in testes expressing a constitutively active EGF Receptor in Drosophila. PMID: 23940622
16. Step acts downstream of the EGFR and is required for the activation of mitogen-activated protein kinase (MAPK) and the induction of EGFR target genes PMID: 23549788
17. this study demonistrated that the EGFR signaling in the brain is necessary for olfactory learning in Drosophila larvae PMID: 23512935
18. Drosophila Hox and sex-determination genes control segment elimination through EGFR and extramacrochetae activity. PMID: 22912593
19. The EGFR pathway acts through bantam to control tissue growth. PMID: 22445297
20. Evidence suggests that Pum functions as a negative regulator of EGFR signaling by directly targeting components of the pathway in Drosophila. PMID: 22514614
21. Findings provide strong evidence that Msk signals non-autonomously through the Vein-Egfr signaling pathway for late tendon cell late differentiation and/or maintenance. PMID: 21925492
22. Egfr signaling is required for specification of alternative neuroblast identities. PMID: 21653613
23. EGFR/Ras/MAPK signaling mediates adult midgut epithelial homeostasis and regeneration in Drosophila PMID: 21167805
24. EGFR pathway coordinates stem cell proliferation and gut remodeling following infection. PMID: 21176204
25. EGFR activation is regulated by transient expression of Rhomboid (Rho), which is required for the maturation of the EGF ligand Spitz. PMID: 20724446
26. survival of a subset of midline glia cells depends upon direct suppression of the proapoptotic protein HID via the EGF receptor/RAS/MAPK pathway PMID: 11832242
27. EGFR signaling is necessary and sufficient to activate apterous (ap) expression, thereby segregating the wing disc into compartments PMID: 11880345
28. mediates cell communication in Drosophila oogenesis PMID: 12015287
29. calcineurin, upon activation by Ca(2+)-calmodulin, cooperates with other factors to negatively regulate Egf receptor signaling at the level of sprouty and the GTPase-activating protein Gap1 PMID: 12019233
30. role of EGFR-Ras signaling in leg patterning PMID: 12114628
31. EGFR signaling plays a role in morphogenesis by modulating cell adhesion PMID: 12163402
32. limb development in flies: the distal region is actually patterned by a distal-to-proximal gradient of RTK activity, established by a source of epidermal growth factor (EGF)-related ligands at the presumptive tip PMID: 12181568
33. Bristles induce bracts via the EGFR pathway on Drosophila legs. PMID: 12204262
34. Pointed and Tramtrack69 establish an EGFR-dependent transcriptional switch to regulate mitosis PMID: 12447387
35. Removing the function of EGFR results in aberrant proliferation and reduced size in the brain lateral to the foregut. PMID: 12646129
36. Data show that Kekkon-1 can interact with mammalian ErbB receptors and that the Kek1/epidermal growth factor receptor interaction inhibits growth factor binding, receptor autophosphorylation and Erk1/2 activation in response to EGF. PMID: 12900463
37. These results indicate that EGFR signaling is crucial for dve expression in the ventral ectoderm and is required in the middle midgut where it cooperates with Dpp signaling. PMID: 14592438
38. EGFR and wingless signaling pathways interact to specify the ocellar pattern in Drosophila PMID: 14656001
39. Suppression of Wg and Egfr activities is an early step in the development of the peripodial epithelium of the wing discs.uppression of Wg and Egfr activities is an early step in the development of the peripodial epithelium of the wing discs. PMID: 14660540
40. Activity of Vein ligand is controlled both positively and negatively, demonstrating the existence of additional levels at which Egfr signaling can be regulated. PMID: 15238521
41. EGFR has a role in recruiting ectodermal attachment cells during the organogenesis of Drosophila proprioceptors PMID: 15296720
42. Argos inhibits DER signalling without interacting directly with the receptor, but instead by sequestering the DER-activating ligand Spitz PMID: 15329724
43. EGF receptor signaling has a role in regulating pulses of cell delamination from the Drosophila ectoderm PMID: 15572130
44. Interactions between Notch, Egfr,and dpp protein signaling pathways regulate vein differentiation during pupal wing development. PMID: 15704120
45. A genome-wide analysis of transcript levels after perturbation of the Egfr pathway in the ovary was used to identify potential Egfr targets in oogenesis. PMID: 15704171
46. contribution of several polymorphisms in the Epidermal growth factor receptor (Egfr) gene to wing shape and size, that were previously mapped in populations of Drosophila melanogaster from North Carolina (NC) and California (CA). PMID: 16102176
47. aveugle is required between ras and raf for EGFR signaling in the eye PMID: 16600911
48. Activity of crossveinless-c, a gene coding for a RhoGAP and whose specific transcriptional activation in the tracheal cells is triggered by both the trachealess patterning gene and the EGF Receptor (EGFR) signaling pathway. PMID: 16818611
49. D-CblS and D-CblL may downregulate Egfr through distinct mechanisms PMID: 16844358
50. Dystroglycan links EGFR-induced repression of the anterior follicle cell fate and anterior-posterior polarity formation in the oocyte PMID: 16908845
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亞細胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Protein kinase superfamily, Tyr protein kinase family, EGF receptor subfamily
組織特異性：

Ubiquitously expressed in embryos. In larvae, uniform expression is seen in wing disks, genital disk, anlagen of testis and ovary, and brain cortex. In eye-antenna disk, highest expression is anterior to morphogenetic furrow, levels remain high in photore
數據庫鏈接：

KEGG: dme:Dmel_CG10079

STRING: 7227.FBpp0071571