Recombinant Drosophila melanogaster Circadian locomoter output cycles protein kaput (Clk), partial

中文名稱：

Recombinant Drosophila melanogaster Circadian locomoter output cycles protein kaput(Clk) ,partial
貨號：

CSB-YP005574DLU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Drosophila melanogaster Circadian locomoter output cycles protein kaput(Clk) ,partial
貨號：

CSB-EP005574DLU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Drosophila melanogaster Circadian locomoter output cycles protein kaput(Clk) ,partial
貨號：

CSB-EP005574DLU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Drosophila melanogaster Circadian locomoter output cycles protein kaput(Clk) ,partial
貨號：

CSB-BP005574DLU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Drosophila melanogaster Circadian locomoter output cycles protein kaput(Clk) ,partial
貨號：

CSB-MP005574DLU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Clk
Uniprot No.：

O61735
別名：

Clk; CLOCK; jrk; PAS1; CG7391Circadian locomoter output cycles protein kaput; dCLOCK; dPAS1
種屬：

Drosophila melanogaster (Fruit fly)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Circadian regulator that acts as a transcription factor and generates a rhythmic output with a period of about 24 hours. Oscillates in antiphase to the cycling observed for period (PER) and timeless (TIM). According to PubMed:9742131, reaches peak abundance within several hours of the dark-light transition at ZT0 (zeitgeber 0), whereas PubMed:9616122 describes bimodal oscillating expression with maximum at ZT5 and ZT23. Clock-cycle heterodimers activate cycling transcription of PER and TIM by binding to the E-box (5'-CACGTG-3') present in their promoters. Once induced, Period and Timeless block Clock's ability to transactivate their promoters.
基因功能參考文獻：
1. experiments define the genetic architecture required to initiate circadian clock function in Drosophila, reveal mechanisms governing circadian activator stability that are conserved in perhaps all eukaryotes, and suggest that Clk, cyc, and cry expression is sufficient to drive clock expression in naive cells PMID: 28973907
2. propose that the dCLK/CYC-controlled TTFL operates differently in subsets of pacemaker neurons, which may contribute to their specific functions PMID: 27489346
3. ClkAR mutants showed significantly faster age-related locomotor deficits. Accelerated locomotor decline of the ClkAR mutant required expression of the PDF receptor and correlated to an apparent loss of dopaminergic neurons in the brain PMID: 28072817
4. these results demonstrate a key role of Clk post-transcriptional control in stabilizing circadian transcription. PMID: 25952406
5. Our findings suggest a novel role for clock protein phosphorylation in governing the relative strengths of entraining modalities by adjusting the dynamics of circadian gene expression. PMID: 25121504
6. These results demonstrate that CLK phosphorylation influences the circadian period by regulating CLK activity and progression through the feedback loop. PMID: 24872414
7. Computational dissection of CLK/CYC context-specific binding sites reveals sequence motifs for putative partner factors, which are predictive for individual binding sites PMID: 24332542
8. usp8 loss of function (RNAi) or expression of a dominant-negative form of the protein (USP8-DN) enhances CLK/CYC transcriptional activity and alters fly locomotor activity rhythms PMID: 23154984
9. CLK has specific targets in different tissues, implying that important CLK partner proteins and/or mechanisms contribute to gene-specific and tissue-specific regulation PMID: 22085964
10. dPER(DeltaCBD) does not provoke the daily hyperphosphorylation of dCLOCK, indicating that direct interactions between dPER and dCLOCK are necessary for the dCLOCK phosphorylation PMID: 20980603
11. findings show that Clk and cyc act during starvation to modulate the conflict of whether flies sleep or search for food PMID: 20541409
12. Here we examined the consequences of loss of clock function on reproductive fitness in Drosophila melanogaster with mutated period (per(0)), timeless (tim(0)), cycle (cyc(0)), and Clock (Clk(Jrk)) genes PMID: 11854509
13. dCLK levels are critical in mediating the direct photostimulation of locomotor activity in Drosophila. PMID: 11931742
14. constitutive levels of nuclear CLK regulate rhythmic transcription in circadian oscillator cells and CLK contributes to other behavioral processes by regulating gene expression in non-oscillator cells PMID: 16603674
15. findings suggest that Clockwork Orange (CWO) acts preferentially in the late night to help terminate CLK-CYC-mediated transcription of direct target genes including cwo itself PMID: 17578907
16. Cwo is rhythmically expressed and directly regulated by CLK-CYC through canonical E-box sequences. PMID: 17578908
17. Results indicate that, in vivo, clockwork orange (CWO) modulates clock gene expression through both repressor and activator transcriptional functions. PMID: 18375860
18. These results define the temporal and spatial coordinates of factors that initiate Clk expression, imply that circadian photoreceptors are not activated until the end of embryogenesis. PMID: 19094242
19. DBT plays a novel noncatalytic role in recruiting additional kinases that phosphorylate CLK, thereby repressing transcription PMID: 19139270
20. the post-translational processing of the circadian CLOCK protein by Cytoplasmic interaction with CYCLE protein PMID: 19376119
21. Sequential phosphorylation and subcellular distribution regulate the activity of the CLK protein. PMID: 19564332
22. Results reveal clear heterogeneity in Clock gene expression in the brain and provide a necessary focus to isolate novel transcription factors that bind at the Clk locus to regulate expression in different oscillator neuron subgroups. PMID: 19755581
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亞細胞定位：

Nucleus.
組織特異性：

Widely expressed. Found in head, body, and appendage fractions.
數據庫鏈接：

KEGG: dme:Dmel_CG7391

STRING: 7227.FBpp0099478

UniGene: Dm.7596