Recombinant Dog Tumor necrosis factor (TNF)

中文名稱：

熟悉的犬TNF重組蛋白
貨號：

CSB-YP023955DO
規(guī)格：
來源：

Yeast
其他：

中文名稱：

熟悉的犬TNF重組蛋白
貨號：

CSB-EP023955DO
規(guī)格：
來源：

E.coli
其他：

中文名稱：

熟悉的犬TNF重組蛋白
貨號：

CSB-EP023955DO-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

熟悉的犬TNF重組蛋白
貨號：

CSB-BP023955DO
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

熟悉的犬TNF重組蛋白
貨號：

CSB-MP023955DO
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

TNF
Uniprot No.：

P51742
別名：

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; N-terminal fragment; NTF); Intracellular domain 1; ICD1); Intracellular domain 2; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor; soluble form]
種屬：

Canis familiaris (Dog) (Canis lupus familiaris)
蛋白長度：

Extracellular domain
表達區(qū)域：

57-233
氨基酸序列

GPQREELPNGLQLISPLAQTVKSSSRTPSDKPVAHVVANPEAEGQLQWLSRRANALLANG VELTDNQLIVPSDGLYLIYSQVLFKGQGCPSTHVLLTHTISRFAVSYQTKVNLLSAIKSP CQRETPEGTEAKPWYEPIYLGGVFQLEKGDRLSAEINLPNYLDFAESGQVYFGIIAL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
基因功能參考文獻：
1. two SNPs of the TNF gene are associated with obesity in Labrador dogs PMID: 26692319
2. Fatal outcome from pulmonary hemorrhages in dogs with leptospirosis was associated with low TNF-alpha, high IL-1beta, and high iNOS expression. PMID: 26824356
3. This diminished TNF-alpha, IL-6 and iNOS expression, and NO production was in parallel to the respective decreased NF-kappaB p65 protein nuclear translocation in the LPS-activated canine PBMCs pretreated with 25 microM and 50 microM propofol. PMID: 25312048
4. Tumour necrosis factor (TNF)-alpha expression was observed in vascular endothelial cells in both neoplastic and normal kidney. PMID: 22300704
5. The observed HMGB1 release kinetics showed a clearly time-dependent manner with a peak release 24h after TNF-alpha stimulation, while stimulation with IFN-gamma had only small effects on the HMGB1 release. PMID: 22154216
6. the TNF-alpha-induced activation of the ERK/GEF-H1/RhoA pathway in tubular cells is mediated through Src- and TACE-dependent EGFR activation. PMID: 21212278
7. The replication characteristics of H3N8 influenza virus and its effects on protein expression of canine alveolar macrophages are reported; only TNF-alpha levels were significantly increased 12 h after inoculation. PMID: 19605916
8. Inflammatory cytokines and NO may be involved in intraneural inflammatory changes arising from mechanical compression PMID: 16022989
9. mRNA and protein structures and identities of the present known mammalian TNFA. PMID: 17573384
10. findings suggest that susceptibility to Anal furunculosis in German Shepherd dogs is primarily associated with DLA-DRB1*00101 and any association with the Tumor Necrosis Factor-alpha locus is secondary and is because of linkage disequilibrium . PMID: 19254251
11. the ERK/GEF-H1/Rho/Rho kinase/phospho-MLC pathway is the mechanism mediating TNF-alpha-induced elevation of tubular epithelial permeability, which in turn might contribute to kidney injury PMID: 19261619
12. The usefulness of HSP27, HSP70, and TNF-alpha as biological markers for canine skin diseases was studied. PMID: 19578946
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亞細胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
數(shù)據(jù)庫鏈接：

KEGG: cfa:403922

STRING: 9615.ENSCAFP00000000739

UniGene: Cfa.54