Recombinant Dog Leptin (LEP)

中文名稱：

熟悉的犬LEP重組蛋白
貨號：

CSB-YP012870DO
規(guī)格：
來源：

Yeast
其他：

中文名稱：

熟悉的犬LEP重組蛋白
貨號：

CSB-EP012870DO
規(guī)格：
來源：

E.coli
其他：

中文名稱：

熟悉的犬LEP重組蛋白
貨號：

CSB-EP012870DO-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

熟悉的犬LEP重組蛋白
貨號：

CSB-BP012870DO
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

熟悉的犬LEP重組蛋白
貨號：

CSB-MP012870DO
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

LEP
Uniprot No.：

O02720
別名：

LEP; OBLeptin; Obesity factor
種屬：

Canis familiaris (Dog) (Canis lupus familiaris)
蛋白長度：

Full Length of Mature Protein
表達(dá)區(qū)域：

22-167
氨基酸序列

VPIRKVQDD TKTLIKTIVA RINDISHTQS VSSKQRVAGL DFIPGLQPVL SLSRMDQTLA IYQQILNSLH SRNVVQISND LENLRDLLHL LASSKSCPLP RARGLETFES LGGVLEASLY STEVVALNRL QAALQDMLRR LDLSPGC
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity. In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides. In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption. Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways. May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression. Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells. Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38. In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses. Increases CD4(+)CD25(-) T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation.
基因功能參考文獻(xiàn)：
1. This is the first report regarding the pattern of leptin during whole pregnancy and lactation. The present work lays the groundwork for further studies on endocrine homeostatic mechanisms using the dog as a model for human medicine PMID: 28583599
2. Leptin and insulin-like growth factor 1 may support spermatogenesis in adult dogs and mediate Leydig cell function PMID: 28101891
3. This study evaluated the differences in leptin, adiponectin, and serotonin between lean and obese dogs. PMID: 24886049
4. These results indicate that both leptin and leptin receptor mRNA are present in the canine corpus luteum during the non-pregnant luteal phase and pregnancy. PMID: 23279462
5. fasting plasma leptin concentration was directly negatively associated with fasting insulin sensitivity and positively associated with beta-cell function PMID: 21474268
6. high-fat feeding induces resistance to the coronary vascular effects of leptin, and this represents an early protective adaptation against endothelial dysfunction PMID: 15894577
7. plasma leptin concentration analysis in dogs PMID: 16919691
8. Leptin dilates canine small mesenteric arteries and veins by a mechanism involving endothelial release of nitric oxide. PMID: 17600555
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Leptin family
數(shù)據(jù)庫鏈接：

KEGG: cfa:403616

STRING: 9615.ENSCAFP00000002433

UniGene: Cfa.3476