Recombinant Botulinum neurotoxin type G (botG), partial

中文名稱：

Recombinant Botulinum neurotoxin type G(botG) ,partial
貨號：

CSB-YP733663CLQ
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Botulinum neurotoxin type G(botG) ,partial
貨號：

CSB-EP733663CLQ
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Botulinum neurotoxin type G(botG) ,partial
貨號：

CSB-EP733663CLQ-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Botulinum neurotoxin type G(botG) ,partial
貨號：

CSB-BP733663CLQ
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Botulinum neurotoxin type G(botG) ,partial
貨號：

CSB-MP733663CLQ
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

botG
Uniprot No.：

Q60393
別名：

botG; Botulinum neurotoxin type G; BoNT/G; Bontoxilysin-G) [Cleaved into: Botulinum neurotoxin G light chain; LC; EC 3.4.24.69); Botulinum neurotoxin G heavy chain; HC)]
種屬：

Clostridium botulinum
蛋白長度：

Partial
表達區域：

2-442
氨基酸序列

PVNIKSFNYNDPINNDDIIMMEPFNDPGPGTYYKAFRIIDRIWIVPERFTYGFQPDQFNASTGVFSKDVYEYYDPTYLKTDAEKDKFLKTMIKLFNRINSKPSGQRLLDMIVDAIPYLGNASTPPDKFAANVANVSINKKIIQPGAEDQIKGLMTNLIIFGPGPVLSDNFTDSMIMNGHSPISEGFGARMMIRFCPSCLNVFNNVQENKDTSIFSRRAYFADPALTLMHELIHVLHGLYGIKISNLPITPNTKEFFMQHSDPVQAEELYTFGGHDPSVISPSTDMNIYNKALQNFQDIANRLNIVSSAQGSGIDISLYKQIYKNKYDFVEDPNGKYSVDKDKFDKLYKALMFGFTETNLAGEYGIKTRYSYFSEYLPPIKTEKLLDNTIYTQNEGFNIASKNLKTEFNGQNKAVNKEAYEEISLEHLVIYRIAMCKPVMYK
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin G which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release. Binds to host peripheral neuronal presynaptic membranes via synaptotagmins 1 and 2 (SYT1 and SYT2). Toxin binds to the membrane proximal extra-cytoplasmic region of SYT1 and SYT2 that is transiently exposed outside of cells during exocytosis; exogenous gangliosides do not enhance binding and subsequent uptake of toxin into host cells. Toxin activity can be blocked by the appropriate synaptotagmin protein fragments in cell culture.; Has proteolytic activity. After translocation into the eukaryotic host cytosol acts as a zinc endopeptidase that cleaves synaptobrevins-1, -2 and -3 (also called VAMP1, VAMP2 and VAMP3). Hydrolyzes the '81-Ala-|-Ala-82' bond of VAMP2, and probably also the equivalent 'Ala-|-Ala' sites in VAMP1 and VAMP3. Has low activity on A.californica synaptobrevin and none on D.melanogaster synaptobrevin or cellubrevin, have a slightly different sequence.; Responsible for host cell targeting and translocation of light chain (LC) into host cytosol. Composed of 3 subdomains; the translocation domain (TD), and N-terminus and C-terminus of the receptor-binding domain (N-RBD, C-RBD). The RBD is responsible for the adherence of the toxin to the cell surface. It simultaneously recognizes 2 coreceptors; polysialated gangliosides and the receptor proteins SYT1 and SYT2 in close proximity on host synaptic vesicles. The N-terminus of the TD wraps an extended belt around the perimeter of the LC, protecting Zn(2+) in the active site; it may also prevent premature LC dissociation from the translocation channel and protect toxin prior to translocation. The TD inserts into synaptic vesicle membrane to allow translocation into the host cytosol. Has 2 coreceptors; complex gangliosides found primarily on neural tissue and host synaptotagmin-1 and -2 (SYT1 and SYT2) which bind to separate sites at the tip of the HC. HC alone binds to host receptors SYT1 and SYT2; C-RBD interacts with host SYT2. Has equal affinity for SYT1 and SYT2; gangliosides are not required for (or only very slightly improve) binding to a membrane-anchored receptor fragment. Has also been shown to only bind SYT1; the assay methods were different. Binds ganglioside GT1b. Significantly decreases uptake and toxicity of whole BoNT/B and BoNT/G.
亞細胞定位：

[Botulinum neurotoxin type G]: Secreted.; [Botulinum neurotoxin G light chain]: Secreted. Host cytoplasm, host cytosol.; [Botulinum neurotoxin G heavy chain]: Secreted. Host cell junction, host synapse, host presynaptic cell membrane. Host cytoplasmic vesicle, host secretory vesicle, host synaptic vesicle membrane; Multi-pass membrane protein.
蛋白家族：

Peptidase M27 family