Recombinant Bacillus subtilis Holliday junction ATP-dependent DNA helicase RuvA (ruvA)
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中文名稱：枯草芽孢桿菌ruvA重組蛋白
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貨號：CSB-EP515380BRJ-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：ruvA
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Uniprot No.：
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別名：ruvA; BSU27740; Holliday junction ATP-dependent DNA helicase RuvA; EC 3.6.4.12
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種屬：Bacillus subtilis (strain 168)
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蛋白長度：full length protein
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表達區域：1-201
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氨基酸序列MIEFVKGTID YVSPQYIVIE NGGIGYQIFT PNPFIYKERS QETIFTYHHI REDAFSLYGF STREEKALFT KLLNVTGIGP KGALAILGSG DPGAVIQAIE NEDEAFLVKF PGVGKKTARQ IILDLKGKLA DVVPEMIENL FNHEERLEKQ TAETALEEAL EALRVLGYAE KEIKKVLPHL KEEIGLTTDQ YVKKALQKLL K
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
靶點詳情
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功能：The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.
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基因功能參考文獻：
- The presence of RuvB.ATPgammaS greatly stimulates RecU-mediated HJ resolution, but the addition of ATP or RuvA abolishes this stimulatory effect. PMID: 24770420
- RuvAB targets RecU to recombination intermediates, and the RuvABC complex is required for repair of double-stranded DNA breaks arising from lesions in chromosomal DNA. PMID: 16020779
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蛋白家族：RuvA family
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數據庫鏈接：
KEGG: bsu:BSU27740
STRING: 224308.Bsubs1_010100015161
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