Recombinant Bacillus subtilis DNA integrity scanning protein DisA (disA)

中文名稱：

枯草芽孢桿菌disA重組蛋白
貨號：

CSB-YP336318BRJ
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

枯草芽孢桿菌disA重組蛋白
貨號：

CSB-EP336318BRJ
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

枯草芽孢桿菌disA重組蛋白
貨號：

CSB-EP336318BRJ-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

枯草芽孢桿菌disA重組蛋白
貨號：

CSB-BP336318BRJ
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

枯草芽孢桿菌disA重組蛋白
貨號：

CSB-MP336318BRJ
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

disA
Uniprot No.：

P37573
別名：

disA; comY; orf6; yacK; BSU00880DNA integrity scanning protein DisA; Cyclic di-AMP synthase; c-di-AMP synthase; Diadenylate cyclase; DAC; EC 2.7.7.85
種屬：

Bacillus subtilis (strain 168)
蛋白長度：

full length protein
表達區域：

1-360
氨基酸序列

MEKEKKGAKH ELDLSSILQF VAPGTPLRAG MENVLRANTG GLIVVGYNDK VKEVVDGGFH INTAFSPAHL YELAKMDGAI ILSDSGQKIL YANTQLMPDA TISSSETGMR HRTAERVAKQ TGCLVIAISE RRNVITLYQE NMKYTLKDIG FILTKANQAI QTLEKYKTIL DKTINALNAL EFEELVTFSD VLSVMHRYEM VLRIKNEINM YIKELGTEGH LIKLQVIELI TDMEEEAALF IKDYVKEKIK DPFVLLKELQ DMSSYDLLDD SIVYKLLGYP ASTNLDDYVL PRGYRLLNKI PRLPMPIVEN VVEAFGVLPR IIEASAEELD EVEGIGEVRA QKIKKGLKRL QEKHYLDRQL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Participates in a DNA-damage check-point that is active prior to asymmetric division when DNA is damaged. Forms globular foci that rapidly scan along the chromosomes during sporulation, searching for lesions. Its ability to scan through the chromosome rapidly is due to its non-specific DNA-binding. When a lesion is present, DisA pauses at the lesion site. This triggers a cellular response that culminates in a temporary block in sporulation initiation. It is required, at least partially, to inhibit the activity of the transcription factor spo0A, which controls, among others, early sporulation genes. In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis; it is toxic when present in excess.; One of 3 paralogous diadenylate cyclases (DAC) in this bacteria. Has diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP acts as a signaling molecule that couples DNA integrity with progression of sporulation. The rise in c-di-AMP level generated by DisA while scanning the chromosome operates as a positive signal that advances sporulation; upon encountering a lesion, the DisA focus arrests at the damaged site and halts c-di-AMP synthesis. Does not convert GTP to c-di-GMP.
基因功能參考文獻：
1. DisA moving along dsDNA indicates absence of DNA damage/replication stress via normal c-di-AMP levels, while interaction with Holliday junction DNA/halted forks leads to reduced c-di-AMP levels and an ensuing block in cell proliferation. RadA/Sms may be involved in modulating DisA activities. PMID: 28511132
2. c-di-AMP is a crucial signalling molecule involved in DNA repair with two diadenylate cyclases, DisA (YbbP) and CdaA (DacA), contributing to modulate different DNA damage responses during exponential growth in Bacillus subtilis cells. PMID: 25616256
亞細胞定位：

Cytoplasm.
蛋白家族：

DisA family
數據庫鏈接：

KEGG: bsu:BSU00880

STRING: 224308.Bsubs1_010100000455