Recombinant Bacillus subtilis Chromosomal replication initiator protein DnaA (dnaA)

中文名稱：

枯草芽孢桿菌dnaA重組蛋白
貨號：

CSB-YP356577BRJ
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

枯草芽孢桿菌dnaA重組蛋白
貨號：

CSB-EP356577BRJ
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

枯草芽孢桿菌dnaA重組蛋白
貨號：

CSB-EP356577BRJ-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

枯草芽孢桿菌dnaA重組蛋白
貨號：

CSB-BP356577BRJ
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

枯草芽孢桿菌dnaA重組蛋白
貨號：

CSB-MP356577BRJ
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

dnaA
Uniprot No.：

P05648
別名：

dnaA; dnaH; BSU00010; Chromosomal replication initiator protein DnaA
種屬：

Bacillus subtilis (strain 168)
蛋白長度：

full length protein
表達區域：

1-446
氨基酸序列

MENILDLWNQ ALAQIEKKLS KPSFETWMKS TKAHSLQGDT LTITAPNEFA RDWLESRYLH LIADTIYELT GEELSIKFVI PQNQDVEDFM PKPQVKKAVK EDTSDFPQNM LNPKYTFDTF VIGSGNRFAH AASLAVAEAP AKAYNPLFIY GGVGLGKTHL MHAIGHYVID HNPSAKVVYL SSEKFTNEFI NSIRDNKAVD FRNRYRNVDV LLIDDIQFLA GKEQTQEEFF HTFNTLHEES KQIVISSDRP PKEIPTLEDR LRSRFEWGLI TDITPPDLET RIAILRKKAK AEGLDIPNEV MLYIANQIDS NIRELEGALI RVVAYSSLIN KDINADLAAE ALKDIIPSSK PKVITIKEIQ RVVGQQFNIK LEDFKAKKRT KSVAFPRQIA MYLSREMTDS SLPKIGEEFG GRDHTTVIHA HEKISKLLAD DEQLQQHVKE IKEQLK
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. DnaA can inhibit its own gene expression as well as that of other genes.
基因功能參考文獻：
1. DnaA shows extremely rapid binding turnover on the chromosome including oriC regions in two bacterial species, which is influenced by Soj and YabA proteins in B. subtilis, and is crucial for balanced initiation control, likely preventing fatal premature multimerization and strand opening of DnaA at oriC. PMID: 28166228
2. Our in vitro characterization of the inherent ability of DnaA to bind the genome at single nucleotide resolution provides a backdrop for interpreting data on in vivo binding and regulation of DnaA PMID: 26020636
3. SirA complex with the N-terminal domain of DnaA and forms heterodimeric complex. PMID: 25041308
4. These findings suggest that DnaA plays several additional roles, such as control of the level of effective initiator, ATP-DnaA, and/or stabilization of the domain structure of the genome around oriC for the proper initiation of chromosome replication. PMID: 17932079
亞細胞定位：

Cytoplasm.
蛋白家族：

DnaA family
數據庫鏈接：

KEGG: bsu:BSU00010

STRING: 224308.Bsubs1_010100000005