Recombinant Bacillus subtilis 2-succinylbenzoate--CoA ligase (menE)

中文名稱：

枯草芽孢桿菌menE重組蛋白
貨號(hào)：

CSB-YP328438BRJ
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

枯草芽孢桿菌menE重組蛋白
貨號(hào)：

CSB-EP328438BRJ
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

枯草芽孢桿菌menE重組蛋白
貨號(hào)：

CSB-EP328438BRJ-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

枯草芽孢桿菌menE重組蛋白
貨號(hào)：

CSB-BP328438BRJ
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

枯草芽孢桿菌menE重組蛋白
貨號(hào)：

CSB-MP328438BRJ
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

menE
Uniprot No.：

P23971
別名：

menE; BSU30790; 2-succinylbenzoate--CoA ligase; EC 6.2.1.26; o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase
種屬：

Bacillus subtilis (strain 168)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-486
氨基酸序列

MLTEQPNWLM QRAQLTPERI ALIYEDQTVT FAELFAASKR MAEQLAAHSV RKGDTAAILL QNRAEMVYAV HACFLLGVKA VLLNTKLSTH ERLFQLEDSG SGFLLTDSSF EKKEYEHIVQ TIDVDELMKE AAEEIEIEAY MQMDATATLM YTSGTTGKPK GVQQTFGNHY FSAVSSALNL GITEQDRWLI ALPLFHISGL SALFKSVIYG MTVVLHQRFS VSDVLHSINR HEVTMISAVQ TMLASLLEET NRCPESIRCI LLGGGPAPLP LLEECREKGF PVFQSYGMTE TCSQIVTLSP EFSMEKLGSA GKPLFSCEIK IERDGQVCEP YEHGEIMVKG PNVMKSYFNR ESANEASFQN GWLKTGDLGY LDNEGFLYVL DRRSDLIISG GENIYPAEVE SVLLSHPAVA EAGVSGAEDK KWGKVPHAYL VLHKPVSAGE LTDYCKERLA KYKIPAKFFV LDRLPRNASN KLLRNQLKDA RKGELL
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl-CoA (OSB-CoA).
基因功能參考文獻(xiàn)：
1. Data suggest that o-succinylbenzoyl-CoA synthetase exhibits a thioesterification active site specifically conserved among orthologues and a substrate-binding mode distinct from those of many other acyl/aryl-CoA synthetases. PMID: 28559280
2. Findings suggest better understand the domain alternation catalytic mechanism of MenE (o-succinylbenzoyl-CoA (OSB-CoA) synthetase) as well as many other adenylating enzymes. PMID: 27933791
3. Structural Basis for the ATP-dependent Configuration of Adenylation Active Site in Bacillus subtilis o-Succinylbenzoyl-CoA Synthetase. PMID: 26276389
蛋白家族：

ATP-dependent AMP-binding enzyme family, MenE subfamily
數(shù)據(jù)庫鏈接：

KEGG: bsu:BSU30790

STRING: 224308.Bsubs1_010100016751