Recombinant Ashbya gossypii Peptide-N (4)- (N-acetyl-beta-glucosaminyl)asparagine amidase (PNG1)

中文名稱：

阿什比亞哥斯比亞PNG1重組蛋白
貨號：

CSB-YP745024DOT
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

阿什比亞哥斯比亞PNG1重組蛋白
貨號：

CSB-EP745024DOT
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

阿什比亞哥斯比亞PNG1重組蛋白
貨號：

CSB-EP745024DOT-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

阿什比亞哥斯比亞PNG1重組蛋白
貨號：

CSB-BP745024DOT
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

阿什比亞哥斯比亞PNG1重組蛋白
貨號：

CSB-MP745024DOT
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

PNG1
Uniprot No.：

Q75D29
別名：

PNG1; ABR193W; Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; PNGase; EC 3.5.1.52; Peptide:N-glycanase 1
種屬：

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
蛋白長度：

full length protein
表達區域：

1-350
氨基酸序列

MRVSEDTSTK EVFERVAADF LQLYKRCVLA QTREVRSSDR ERCEELVRRN ALARELCKLH QTLCFVYEND ALYGIVLDAL DLEGIYGRVE EGPGADDYQD RLVQELLRYF KDEFFTWCDK PLCARCGTAK KQAAVGHGKP TVEEARYRCT VVELFRCEDC GDVARFPRYN DPLKLLETRT GRCGEWCNLF MLILRSFGIE ARYTWNREDH VWCEVYSNAL KRWVHVDSCE KSFDEPHIYS VNWNKAMSYV IAFSNRSVKD VSRRYIVRNR LPRDQIDEDD LQFLTKYLTK LLRLQLPDEE RYLLHCRDEL EAIDLLGSKT APMEIPPAAG AAGRQSGSAD WKRQRGEDGR
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.
亞細胞定位：

Cytoplasm.
蛋白家族：

Transglutaminase-like superfamily, PNGase family
數據庫鏈接：

KEGG: ago:AGOS_ABR193W

STRING: 33169.AAS50966