Recombinant Arabidopsis thaliana Transcription factor HY5 (HY5)

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中文名稱：

擬南芥HY5重組蛋白
貨號(hào)：

CSB-EP519576DOA
說明書：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

HY5
Uniprot No.：

O24646
種屬：

Arabidopsis thaliana (Mouse-ear cress)
蛋白長度：

Full Length
來源：

E.coli
分子量：

25.9 kDa
表達(dá)區(qū)域：

1-168aa
氨基酸序列

MQEQATSSLAASSLPSSSERSSSSAPHLEIKEGIESDEEIRRVPEFGGEAVGKETSGRESGSATGQERTQATVGESQRKRGRTPAEKENKRLKRLLRNRVSAQQARERKKAYLSELENRVKDLENKNSELEERLSTLQNENQMLRHILKNTTGNKRGGGGGSNADASL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Transcription factor that promotes photomorphogenesis in light. Acts downstream of the light receptor network and directly affects transcription of light-induced genes. Specifically involved in the blue light specific pathway, suggesting that it participates in transmission of cryptochromes (CRY1 and CRY2) signals to downstream responses. In darkness, its degradation prevents the activation of light-induced genes (Probable). Acts coordinately with SPL7 to regulate the microRNA miR408 and its target genes in response to changes in light and copper conditions. Regulates the abscisic acid (ABA) signaling pathway. Also involved in root gravitropism. Involved in the repression of hypocotyl cell elongation to promote photomorphogenesis. Recruits the histone deacetylase HDA15 to the promoters of a subset of cell wall organization and auxin signaling-related genes. HDA15 represses their transcription by decreasing the levels of histone H4 acetylation in a light-dependent manner.
基因功能參考文獻(xiàn)：
1. Results suggest that light increases the endoplasmic reticulum stress sensitivity of plants and ELONGATED HYPOCOTYL 5, a positive regulator of light signaling, negatively regulates unfolded protein response gene expression in plant cells. PMID: 28167764
2. Repression of MYBL2 by Both microRNA858a and HY5 Leads to the Activation of Anthocyanin Biosynthetic Pathway in Arabidopsis PMID: 27450422
3. Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis thaliana by Promoting Nuclear Proteasome-Mediated HY5 Degradation PMID: 28412546
4. BBX21 binds to the T/G-box in the HY5 promoter and directly activates HY5 expression in the light. PMID: 27325768
5. Arabidopsis ELONGATED HYPOCOTYL5 (HY5) is a shoot-to-root mobile signal that mediates light promotion of root growth and nitrate uptake. Shoot-derived HY5 auto-activates root HY5 and also promotes root nitrate uptake by activating NRT2.1, a gene encoding a high-affinity nitrate transporter. PMID: 26877080
6. This study showed that HY5 directly binds to the MYBD promoter and that although MYBD and MYBH are homologs, they act in opposite ways during plant photomorphogenesis. PMID: 26576746
7. the physical connection between brassinosteroid and light signaling pathways in regulating cotyledon opening in Arabidopsis is mediated by a direct interaction between BZR1 and HY5 PMID: 26363272
8. Compromised activity of HY5 may have been mainly responsible for the partial reversal of the det1 phenotype in ted3 det1. PMID: 25248106
9. Ultraviolet-B causes unilateral accumulation of HY5, which then, in the end, leads to bending toward the light. PMID: 25096978
10. Data indicate that the bZIP transcription factor ELONGATED HYPOCOTYL5 (HY5) is enriched at target promoters in response to UV-B in a UV RESISTANCE LOCUS8 (UVR8) photoreceptor-dependent manner. PMID: 25351492
11. by directly targeting a common promoter cis-element (G-box), HY5 and PIFs form a dynamic activation-suppression transcriptional module responsive to light and temperature cues. PMID: 24922306
12. our investigation demonstrates that the COP1-HY5 complex is a novel integrator that plays an essential role in ethylene-promoted hypocotyl growth in the light PMID: 24348273
13. A possible interplay between PFT1 and another transcription factor, HY5, may regulate induction of APS reductase expression by light. PMID: 24583010
14. HY5 transmits phyA signals through an FHY1/FHL-independent pathway but it may also modulate FHY1/FHL signal through its interaction with HFR1 and LAF1. PMID: 23503597
15. Bifurcate regulation of anthocyanin biosynthesis by HY5 via transcriptional activation of PAP1. PMID: 23583450
16. The HY5-AtERF11 regulon is a key factor modulating abscisic acid-regulated ethylene biosynthesis. PMID: 21645149
17. HSP90 proteins respond to the tetrapyrrole-mediated plastid signal to control expression of photosynthesis-associated nuclear genes PMID: 22201048
18. HY5 and BBX24 act antagonistically in UV-B response. PMID: 22410790
19. HY5 binds to over 9000 genes, detectably affecting the expression of over 1100 genes, either positively or negatively. HY5 indirectly regulate many other genes through sub-networks mediated by other regulators. PMID: 21265889
20. HY5 represses the expression of PKS4 and auxin-related genes. PMID: 21848684
21. The genetic interaction between HY5 and FRY1 indicates that HY5 and FRY1 may act in overlapping pathways that mediate light signaling and lateral root development. PMID: 21301222
22. HY5 directly binds to C/G-box and G-box in the HTL promoter and regulates the expression of HTL. PMID: 20864454
23. Data show that HY5 plays a role in negative feedback regulation of phyA signaling by attenuating FHY3/FAR1-activated FHY1/FHL expression, providing a mechanism for fine-tuning phyA signaling homeostasis. PMID: 21097709
24. Data show that phosphorylation-mimicking serine substitutions strongly interfere with the DNA binding of two prototypical Arabidopsis bZIPs, namely AtZIP63 and HY5. PMID: 20047775
25. Det1 esp1 spa1 double mutant showed higher HY5 protein levels than either single mutant or the wild type. PMID: 20041285
26. HY5 is required for the transcriptional activation of the PFG1/MYB12 and PFG3/MYB111 genes under UV-B and visible light. PMID: 19895401
27. the first structural and biophysical characterization of HY5 is reported here. PMID: 17001643
28. HY5 is a high hierarchical regulator of the transcriptional cascades for photomorphogenesis. PMID: 17337630
29. necessary for high nitrate reductase expression in far-red light PMID: 17929051
30. Gibberellins signaling regulates protein stability of HY5, and the activity of PIF3. PMID: 18053005
31. Data show that at part of the remodeling of light signaling networks involves converting HY5, a positive regulator of PhANGs, into a negative regulator of PhANGs. PMID: 18065688
32. Arabidopsis HY5 functions as a novel DNA-binding tag (DBtag) for proteins. We also demonstrate that the DBtagged proteins could be immobilized and purified on a newly designed agarose/DNA microplate PMID: 18082144
33. The isolation and characterization of light-regulated zinc finger protein 1 (LZF1), a HY5-regulated factor, is reported. PMID: 18182030
34. action spectrum for the induction of HY5 by the UVR8 pathway showed a main peak at 280 nm with a smaller peak at 300 nm PMID: 19558421
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亞細(xì)胞定位：

Nucleus.
蛋白家族：

BZIP family
組織特異性：

Expressed in root, hypocotyl, cotyledon, leaf, stem and floral organs.
數(shù)據(jù)庫鏈接：

KEGG: ath:AT5G11260

STRING: 3702.AT5G11260.1

UniGene: At.20257