Recombinant Arabidopsis thaliana Phytochrome A (PHYA), partial

中文名稱：

擬南芥PHYA重組蛋白
貨號：

CSB-YP320415DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

擬南芥PHYA重組蛋白
貨號：

CSB-EP320415DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

擬南芥PHYA重組蛋白
貨號：

CSB-EP320415DOA-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

擬南芥PHYA重組蛋白
貨號：

CSB-BP320415DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

擬南芥PHYA重組蛋白
貨號：

CSB-MP320415DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

PHYA
Uniprot No.：

P14712
別名：

PHYA; FHY2; FRE1; HY8; At1g09570; F14J9.23; Phytochrome A; Protein ELONGATED HYPOCOTYL 8; Protein FAR RED ELONGATED 1; Protein FAR RED ELONGATED HYPOCOTYL 2
種屬：

Arabidopsis thaliana (Mouse-ear cress)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenetic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses. Pfr controls the expression of a number of nuclear genes including those encoding the small subunit of ribulose-bisphosphate carboxylase, chlorophyll A/B binding protein, protochlorophyllide reductase, rRNA, etc. It also controls the expression of its own gene(s) in a negative feedback fashion. Involved in the flowering time regulation. Can phosphorylate FHY1 and, possibly, FHL, in red light conditions; this inactivates their co-shuttling to the nucleus. Regulates phototropic responses both in the nucleus (e.g. hypocotyl elongation and cotyledon opening under high-irradiance conditions and seed germination under very-low-fluence conditions) and in the cytoplasm (e.g. negative gravitropism in blue light and red-enhanced phototropism).
基因功能參考文獻(xiàn)：
1. phyA modulates the abundance and activity of key regulatory transcription factors in a tissue-autonomous fashion. PMID: 27027866
2. these results suggest that lysine 206 is the main lysine for rapid ubiquitination and protein degradation of Arabidopsis phytochrome A. PMID: 26314334
3. phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis PMID: 25009301
4. PHYA, but not PHYB, is essential for fine-tuning the chlorophyll biosynthetic pathway in response to partial shading. PMID: 24604733
5. nuclear FHY1 can either act independently of phyA or act in association with phyA to activate the expression of distinct target genes PMID: 25071219
6. phytochrome A function in Arabidopsis seed germination PMID: 23292879
7. Distinct structural modules, each of which confers different properties to phyA, are assembled on the phyA molecule. PMID: 22843485
8. We present here a hypothesis to explain how the identified amino acid substitution may lead to structural changes manifested as altered signaling and phenotype displayed by the phyA-5 mutant (which shows reduced binding affinity to FHY1/FHL). PMID: 22516823
9. phyA controlles cry2 stability PMID: 22739826
10. The truncated PHYA406 proteins inactivate COP1 in the nuclei in a light-independent fashion. PMID: 22498774
11. Nuclear phyA accelerates phototropism. In the fhy1 fhl mutant, in which phyA remains in the cytosol, phototropic bending is slower than in the wild type. PMID: 22374392
12. PHYA amino-terminal extension domain plays a role in regulating the nuclear import of PHYA. PMID: 21969386
13. hypomethylation at a specific CG site in exon 1 was consistently associated with the release of phyA' epiallele silencing. PMID: 22466452
14. A role for mesophyll-specific phyA in blue-light-dependent regulation of anthocyanin levels and novel roles for individual phy isoforms in the regulation of anthocyanin accumulation under red illumination, is reported. PMID: 21455024
15. Study unravels the mechanism underlying this shift of the phyA action peak from red to far-red light and show that it relies on specific molecular interactions rather than on intrinsic changes to phyA's spectral properties. PMID: 21884939
16. The dracula 1 (dra1) mutant, that showed no avoidance of shade for the PHYB::LUC response, was the result of a mutation in the PHYA gene. PMID: 21398429
17. Reversible activation and repression of phyA expression by darkness and light, respectively, is accompanied by reversible changes in H3 and H4 modifications of the phyA locus. PMID: 21317377
18. The phyA N-terminal fragment is imported into the nucleus in a light-dependent fashion. Deletion of the C-terminal domain of phyA compromises light-induced degradation of phyA in a compartmentalization-independent fashion. PMID: 21169346
19. phyA without a nuclear localization or export signal sequence is localized exclusively in the cytoplasm in darkness. Rapid nuclear entry was observed after exposure to both red and far-red light. PMID: 20739301
20. phyA regulates wound- and jasmonic acid-mediated JAZ1 degradation. PMID: 20435902
21. Data show that phyA degradation is significantly slower in the cytoplasm than in the nucleus. PMID: 20473552
22. Results suggest that the autophosphorylation of phyA plays an important role in the regulation of plant phytochrome signaling through the control of phyA protein stability. PMID: 20203237
23. Genetic variations in the PhyA locus of A. thaliana inbred strains and the effects on seedling growth under infrared light are reported. PMID: 15908601
24. The potential of manipulating light signal-transduction pathways to minimize the problems of lodging in basmati/aromatic rice and to enhance grain productivity is reported. PMID: 16136335
25. data indicate that phyA, the 26S proteasome, and the Constitutive Photomorphogenic/De-Etiolated/Fusca proteins are all involved in the light regulation of FHY1 protein abundance during Arabidopsis (Arabidopsis thaliana) seedling development PMID: 16244150
26. PHYC was the most strongly associated locus across 163 strains, suggesting that PHYC alleles are under diversifying selection in A. thaliana PMID: 16732287
27. This study showed that phyA has a quantitatively dominant role in Rc-induced expression of early response genes in A. thaliana seedlings. PMID: 17076805
28. The serine-rich region is involved in negative regulation of phyA signaling. PMID: 17160561
29. Results raise the possibility that phyA may contribute significantly to the regulation of growth and development in daylight-grown plants. PMID: 17346261
30. Data showed a transcriptionally suppressed epi-allele of phytochrome A gene (PHYA) methylated only in symmetric CG sites resident in exonic regions, resulting in elongated hypocotyls in seedlings grown under continuous far-red light. PMID: 17931351
31. required for the up-regulation of PEX11b in the light PMID: 18203870
32. The expression levels of polyamine biosynthesis-related genes were wtranscribed less in the wild type than in phyA seedlings under both light conditions. PMID: 18375607
33. Data show that phyA plays a dominant role in regulating the degradation of PIF1 following initial light exposure and PIF1 interacts with phyA through a novel active phyA binding motif. PMID: 18539749
34. FHY1 and FHL work as adaptor proteins facilitating nuclear transport of phyA. PMID: 18670649
35. Phytochrome A mediates the localization of phot1 in Arabidopsis. PMID: 18952772
36. The G-protein may be involved in the phyA signaling pathway to regulate far-red irradiation-preconditioned cell death of Arabidopsis hypocotyls. PMID: 19160542
37. Phytochrome A is required for the phosphorylation of FHY1. PMID: 19208901
38. A missense mutation in the PHYA histidine kinase-related domain alters the spectral sensitivity and the persistence of far-red light-induced high-irradiance responses. PMID: 19403732
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亞細(xì)胞定位：

Cytoplasm. Nucleus, nucleoplasm. Nucleus speckle.
蛋白家族：

Phytochrome family
數(shù)據(jù)庫鏈接：

KEGG: ath:AT1G09570

STRING: 3702.AT1G09570.1

UniGene: At.22828