Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 1 [UDP-forming] (CESA1) , partial

^{In
Stock}

中文名稱：

Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 1 [UDP-forming] (CESA1) , partial
品名簡稱：

Recombinant Mouse-ear cress CESA1 protein, partial
貨號：

CSB-EP525056DOA
說明書：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

CESA1
Uniprot No.：

O48946
別名：

AtCesA1;Protein RADIALLY SWOLLEN 1;AtRSW1
種屬：

Arabidopsis thaliana (Mouse-ear cress)
蛋白長度：

Partial
來源：

E.coli
分子量：

61.1 kDa
表達區(qū)域：

321-856aa
氨基酸序列

DQFPKWYPINRETYLDRLAIRYDRDGEPSQLVPVDVFVSTVDPLKEPPLVTANTVLSILSVDYPVDKVACYVSDDGSAMLTFESLSETAEFAKKWVPFCKKFNIEPRAPEFYFAQKIDYLKDKIQPSFVKERRAMKREYEEFKVRINALVAKAQKIPEEGWTMQDGTPWPGNNTRDHPGMIQVFLGHSGGLDTDGNELPRLIYVSREKRPGFQHHKKAGAMNALIRVSAVLTNGAYLLNVDCDHYFNNSKAIKEAMCFMMDPAIGKKCCYVQFPQRFDGIDLHDRYANRNIVFFDINMKGLDGIQGPVYVGTGCCFNRQALYGYDPVLTEEDLEPNIIVKSCCGSRKKGKSSKKYNYEKRRGINRSDSNAPLFNMEDIDEGFEGYDDERSILMSQRSVEKRFGQSPVFIAATFMEQGGIPPTTNPATLLKEAIHVISCGYEDKTEWGKEIGWIYGSVTEDILTGFKMHARGWISIYCNPPRPAFKGSAPINLSDRLNQVLRWALGSIEILLSRHCPIWYGYHGRLRLLERIAYINT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation. Involved in the primary cell wall formation. Required during embryogenesis for cell elongation, orientation of cell expansion and complex cell wall formations, such as interdigitated pattern of epidermal pavement cells, stomatal guard cells and trichomes. Plays a role in lateral roots formation, but seems not necessary for the development of tip-growing cells such as root hairs. The presence of each protein CESA1 and CESA6 is critical for cell expansion after germination.
基因功能參考文獻：
1. AtCesA6-null mutants showed a reduced cell elongation of young seedlings with little impact on cell division, which consequently affected cell wall integrity and biomass yield of mature plants. In comparison, rsw1 seedlings exhibited a strong defect in both cell elongation and division at restrictive temperature, whereas the IRX3 mutant showed normal seedling growth. PMID: 29514326
2. Our data support a model in which distinct spatiotemporal features of active CSCs (cellulose synthase (CESA) complex))during PCW and SCW synthesis contribute to the formation of cellulose with distinct structure and organization in PCWs and SCWs of Arabidopsis thaliana PMID: 27647923
3. microscopy showed that two altered versions of Atcesa1 could rescue cell wall phenotypes observed in the mutant background line. Overall, the data show that the FxVTxK motif is functionally important in two phylogenetically distant plant CESAs. PMID: 26646446
4. A low resolution structure of the catalytic domain of CESA1 determined by small-angle scattering techniques provides the first experimental evidence for the self-assembly of CESA1 into a stable trimer. PMID: 26556795
5. The stoichiometry of the cellulose synthase catalytic subunits (CESAs) in the primary cell wall CSC was determined and CESA1, CESA3, and CESA6 are present in a 1:1:1 molecular ratio. PMID: 25352273
6. computational findings on transmembrane helices (TMHs) 5 and 6 in three CESAs of Arabidopsis thaliana (AtCESA1, 3, and 6), the Atcesa3(ixr1-2) mutant, and a novel missense mutation in AtCESA1, are reported. PMID: 25262226
7. Relatively high sequence conservation between plant CESAs allowed mapping of known mutations and two previously undescribed mutations that perturb cellulose synthesis in Arabidopsis thaliana to their analogous positions in the modeled structure. PMID: 23592721
8. CESA1(A903V) and CESA3(T942I) have modified microfibril structure in terms of crystallinity and suggest that in plants, as in bacteria, crystallization biophysically limits polymerization. PMID: 22375033
9. Data suggest that phosphorylation of CESA1 affects polar interactions with microtubules that may regulate the length or quantity of a subset of cellulose microfibrils and that this, in turn, alters microfibril structure in the primary cell wall. PMID: 20855602
10. The effects of CESA1 and CESA3 mutations and chimeric proteins on the temperature sensitivity of A. thaliana is reported. PMID: 16891551
11. CESA 1, 3, and 6 form a complex which is important for cellulose synthesis. PMID: 18495638
顯示更多

收起更多
亞細胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

Glycosyltransferase 2 family, Plant cellulose synthase subfamily
組織特異性：

Expressed in germinating seeds, seedlings, roots, stems, shoots leaves and flowers, but not in mature flowers.
數(shù)據(jù)庫鏈接：

KEGG: ath:AT4G32410

STRING: 3702.AT4G32410.1

UniGene: At.21246