UQCRC2 Recombinant Monoclonal Antibody

說明書

中文名稱：

UQCRC2重組抗體
貨號：

CSB-RA229127A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in:HepG2 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate
All lanes: UQCRC2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa

IHC image of CSB-RA229127A0HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA229127A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA229127A0HU UQCRC2重組單克隆抗體是針對線粒體復合體III核心亞基2（UQCRC2）研發的高特異性檢測工具。該抗體識別的靶蛋白作為線粒體呼吸鏈復合體III的重要組成部分，參與細胞能量代謝中的電子傳遞與氧化磷酸化過程，在維持線粒體功能穩態及調控細胞凋亡中發揮關鍵作用。經嚴格驗證，本產品在Western Blot實驗中可清晰檢測到特異性目標條帶，且IHC實驗能精準定位線粒體內源表達信號，推薦使用稀釋度為WB 1:500-1:5000、IHC 1:50-1:200。其卓越的批次間穩定性和低交叉反應特性，可滿足細胞裂解物、組織勻漿等多種樣本類型的檢測需求，尤其適用于探究線粒體動態調控、能量代謝異常機制、氧化應激響應等基礎研究領域。科研人員可利用該抗體開展蛋白質表達分析、亞細胞定位研究及疾病相關分子通路探索，為神經退行性疾病、代謝綜合征等病理機制的體外研究提供可靠工具。
Uniprot No.：

P22695
基因名：

UQCRC2
別名：

Cytochrome b-c1 complex subunit 2, mitochondrial (Complex III subunit 2) (Core protein II) (Ubiquinol-cytochrome-c reductase complex core protein 2), UQCRC2
反應種屬：

Human
免疫原：

A synthesized peptide derived from human UQCRC2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

2F6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties. May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable).
基因功能參考文獻：
1. These results provided novel insights into the potential role of UQCRC2 in the tumorigenesis and progression of colorectal cancer PMID: 30115536
2. Down-regulation of UQCRC2 partly reversed the inhibition of invasion/migration ability and chemoresistance in CDH18 overexpressed glioma cell lines. PMID: 30078018
3. These data indicate that a homozygous missense mutation in UQCRC2 causes moderately impaired CIII function and severely decreased amounts of CIII and supercomplex, which would be the primary molecular pathogenesis in the patients. PMID: 23281071
相關疾病：

Mitochondrial complex III deficiency, nuclear 5 (MC3DN5)
亞細胞定位：

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
蛋白家族：

Peptidase M16 family, UQCRC2/QCR2 subfamily
數據庫鏈接：

HGNC: 12586

OMIM: 191329

KEGG: hsa:7385

STRING: 9606.ENSP00000268379

UniGene: Hs.528803

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