VAMP8 Recombinant Monoclonal Antibody

說明書

中文名稱：

VAMP8 Recombinant Monoclonal Antibody
貨號：

CSB-RA846974A0HU
規(guī)格：

￥1320
圖片：
Flow cytometric analysis of Vesicle associated membrane protein 8 expression in HepG2 cells using Vesicle associated membrane protein 8 antibody. Green, isotype control; red, Vesicle associated membrane protein 8.

Immunocytochemical staining of HepG2 cells with vesicle associated membrane protein 8 antibody. Nuclei were stained blue with DAPI; Vesicle associated membrane protein 8 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using vesicle associated membrane protein 8 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with vesicle associated membrane protein 8 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

Uniprot No.：

Q9BV40
基因名：

VAMP8
別名：

VAMP8; Vesicle Associated Membrane Protein 8; VAMP-8; EDB; Vesicle-Associated Membrane Protein 8; Endobrevin; Vesicle-Associated Membrane Protein 8 (Endobrevin)
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human VAMP8 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1F8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism.
基因功能參考文獻：
1. our study suggested that VAMP8 gene variants might not contribute to glioma susceptibility and associated with glioma in the Chinese Han population. PMID: 26191281
2. Vesicle-associated membrane protein 8 as a novel oncogene by promoting cell proliferation and therapeutic resistance in glioma. Targeting VAMP8 may serve as a potential therapeutic regimen for the treatment of glioma. PMID: 25209430
3. Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 to promote autophagosome-lysosome fusion. PMID: 25648148
4. Cytotoxic granule exocytosis is a sequential, multivesicle fusion process requiring VAMP8-mediated recycling endosome fusion before cytotoxic granule fusion. PMID: 26124288
5. Inhibition of VAMP8, but not VAMP7, significantly reduces viral entry. PMID: 24550300
6. VAMP8 regulates mucin granule exocytosis in airway goblet cells, and reduction of its expression may provide a novel therapeutic target to ameliorate airway mucus obstruction in lung diseases PMID: 22144578
7. Assessment of KIF6 genotype is not useful in predicting low density lipoprotein cholesterol lowering response to pravastatin, and heart disease risk reduction in the elderly. PMID: 22192511
8. The VAMP8 rs1010 polymorphism was associated with CHD risk in Chinese Han population, the A allele might serve as a genetic risk factor of coronary heart disease. PMID: 20533276
9. Results suggest that the combinational SNARE proteins VAMP8 and Vti1b mediate the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential event for autophagic degradation. PMID: 20089838
10. Support a role for VAMP8/endobrevin in the heterogeneity of platelet reactivity, and suggest a role for microRNA-96 in the regulation of VAMP8 expression. PMID: 19943878
11. VAMP8 is not essential for constitutive exocytosis, although it can be involved in the exocytosis PMID: 19738360
12. VAMP-3 and VAMP-8 are present in human platelets and are required for granule secretion. PMID: 12130530
13. Our findings indicate that VAMP5 and VAMP8 are not involved in POAG in the Dutch population. PMID: 16110299
14. VAMP-8 is unlikely to be a v-SNARE for constitutive exocytosis in HSY cells. PMID: 16195891
15. Variants in 2 genes were associated with early-onset myocardial infarct: VAMP8, which is involved in platelet degranulation, and HNRPUL1, which encodes a ribonuclear protein. PMID: 16690874
16. Data show that mature human mast cells express a specific pattern of SNARE and that VAMP-7 and VAMP-8, but not VAMP-2, are required for rapid degranulation. PMID: 18253931
17. A putative association between a VAMP8 gene variant and platelet functions ex vivo, was tested. PMID: 19374688
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亞細胞定位：

Lysosome membrane; Single-pass type IV membrane protein. Early endosome membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein. Cell membrane; Single-pass type IV membrane protein. Zymogen granule membrane; Single-pass type IV membrane protein.
蛋白家族：

Synaptobrevin family
組織特異性：

Platelets.
數(shù)據(jù)庫鏈接：

HGNC: 12647

OMIM: 603177

KEGG: hsa:8673

STRING: 9606.ENSP00000263864

UniGene: Hs.714302

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