TUBB1 Recombinant Monoclonal Antibody
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中文名稱：TUBB1重組抗體
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貨號：CSB-RA867148MA1HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: TUBB1 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa -
IHC image of CSB-RA867148MA1HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA867148MA1HU diluted at 1:300 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. -
Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA867148MA1HU (red line) at 1:200. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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產品描述：CSB-RA867148MA1HU TUBB1重組單克隆抗體是針對β-微管蛋白家族成員TUBB1開發的高特異性檢測工具。該抗體靶向的TUBB1蛋白是細胞骨架的核心組成單元，在維持細胞形態、有絲分裂及細胞內物質運輸中發揮重要作用，其功能異常與巨核細胞成熟障礙及血小板生成異常密切相關。經多種技術平臺驗證，本抗體在Western Blot中可清晰識別內源性TUBB1蛋白，免疫組化實驗顯示其在細胞質中的特異性定位，流式細胞分析進一步證實其對細胞表面微管結構的識別能力。推薦的實驗優化濃度范圍為：免疫印跡1:1000-5000，組織切片染色1:20-200，流式檢測1:20-200，適用于細胞骨架動態研究、血小板分化機制探索以及血液系統疾病模型的構建。產品經過嚴格的質量控制，在不同實驗體系中均表現出優異的批間一致性和信號穩定性，為研究微管蛋白功能調控及相關病理機制提供可靠工具。
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Uniprot No.：
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基因名：
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別名：2810484G07Rik antibody; beta 1 tubulin antibody; Beta tubulin 1, class VI antibody; Beta-tubulin antibody; Class VI beta tubulin antibody; dJ543J19.4 antibody; M(beta)1 antibody; TBB1_HUMAN antibody; TUBB1 antibody; Tubulin beta 1 class VI antibody; Tubulin beta-1 chain antibody; Tubulin, beta 1 antibody; tubulin, beta1 antibody
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反應種屬：Human
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免疫原：Recombinant Human TUBB1 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Mouse IgG
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純化方式：Affinity-chromatography
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克隆號：20B1
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 IHC 1:20-1:200 FC 1:20-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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基因功能參考文獻：
- Neonatal platelets exhibit low levels of the Stx11-Munc18b complex (essential component of the SNARE machinery) and of beta1-tubulin. These developmental deficiencies are associated with defects in platelet adhesion, spreading and secretion. PMID: 29044293
- novel DCX mutation (p.D90G, NP_000546.2) appeared to be a major causative variant, whereas the novel mutation of TUBB1 (p.R62fsX, NP_110400.1) was found only in patients with more-severe intellectual disability in Familial pachygyria PMID: 26743950
- Analysis of the TUBB1 gene revealed three known missense variants in heterozygous state which in combination might explain the beta1-tubulin defect. PMID: 26540125
- TUBB1 R307H SNP is significantly associated with the degree of thrombocytopenia in congenital and acquired platelet disorders, and may affect platelets by altering microtubule behavior. PMID: 25529050
- Data indicate that ABCB1 protein, beta tubulin I and III (betaI, and betaIII tubulin) might contribute to the multidrug resistance (MDR) of MCF7/DOC and be potential therapeutic targets for overcoming MDR of breast cancer. PMID: 24894670
- TUBB1 mutation disrupting microtubule assembly impairs proplatelet formation and results in congenital macrothrombocytopenia. PMID: 24344610
- our findings define beta-tubulin VI as a hematologic isotype with significant genetic variation in humans that may affect the myelosuppresive action of microtubule-binding drugs PMID: 22805305
- homozygous status of P43 genetic polymorphism causes alterations in platelet ultrastructure PMID: 21384078
- Studies show that BFBTS bound and modified beta-tubulin at residue Cys12, forming beta-tubulin-SS-fluorobenzyl. PMID: 19996274
- SLPI localizes in part along the megakaaryocyte and platelet cytoskeleton by virtue of specific interactions with beta1 tubulin. PMID: 15315966
- the platelet Q43P beta1-tubulin substitution is frequent in the healthy population and may protect men against arterial thrombosis PMID: 15956286
- The TUBB1 Q43P polymorphism, by causing a lower reactivity in platelets carrying the variant form of b1-tubulin, protects against thrombotic disorders but increases the risk of intracerebral hemorrhage in men. PMID: 17488662
- biophysical analysis of carboxy-terminal tail conformation of human beta-tubulin isotypes PMID: 17993481
- Mutation of the beta1-tubulin gene associated with congenital macrothrombocytopenia affecting microtubule assembly. PMID: 18849486
- TUBB1 Q43P polymorphism does not protect against acute coronary syndrome and premature myocardial infarction. PMID: 19132255
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相關疾病：Macrothrombocytopenia, autosomal dominant, TUBB1-related (MAD-TUBB1)
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亞細胞定位：Cytoplasm, cytoskeleton.
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蛋白家族：Tubulin family
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組織特異性：Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins.
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數據庫鏈接：
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