OS9 Recombinant Monoclonal Antibody
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中文名稱：OS9重組抗體
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貨號：CSB-RA959292A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: HL-60 whole cell lysate, Hela whole cell lysate, Mouse kidney tissue
All lanes: OS9 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76, 70, 68, 74, 66, 64, 70, 61 kDa
Observed band size: 72-95 kDa -
IHC image of CSB-RA959292A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA959292A0HU OS9重組單克隆抗體是針對內(nèi)質(zhì)網(wǎng)相關(guān)降解通路關(guān)鍵調(diào)控因子OS9蛋白開發(fā)的高特異性科研工具。該抗體識別的OS9蛋白在細(xì)胞內(nèi)負(fù)責(zé)介導(dǎo)錯誤折疊蛋白的識別與降解調(diào)控，其異常表達(dá)與多種細(xì)胞應(yīng)激反應(yīng)及病理過程密切相關(guān)。經(jīng)多平臺驗(yàn)證顯示，該抗體在Western Blot實(shí)驗(yàn)中可清晰檢測目標(biāo)蛋白表達(dá)，在免疫組織化學(xué)應(yīng)用中能有效顯示組織切片中OS9的亞細(xì)胞定位特征，同時在ELISA體系中也表現(xiàn)出優(yōu)異的抗原結(jié)合能力。推薦使用稀釋度為WB 1:500-1:2000、IHC 1:50-1:200，實(shí)驗(yàn)數(shù)據(jù)顯示其在不同樣本類型中均保持穩(wěn)定的檢測靈敏度和低背景干擾。該重組單克隆抗體采用哺乳動物表達(dá)系統(tǒng)制備，具有高度均一的批次穩(wěn)定性，適用于研究蛋白質(zhì)質(zhì)量控制機(jī)制、內(nèi)質(zhì)網(wǎng)應(yīng)激相關(guān)信號通路以及腫瘤等疾病模型中異常蛋白代謝的分子機(jī)制，為探索細(xì)胞穩(wěn)態(tài)調(diào)控及疾病相關(guān)分子網(wǎng)絡(luò)提供可靠的研究工具。
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Uniprot No.：
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基因名：
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別名：Protein OS-9 (Amplified in osteosarcoma 9), OS9
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反應(yīng)種屬：Human, Mouse
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免疫原：A synthesized peptide derived from human OS9
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：26H3
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
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基因功能參考文獻(xiàn)：
- The endoplasmic reticulum-associated protein, OS-9, behaves as a lectin in targeting the immature calcium-sensing receptor. PMID: 28419469
- OS-9 up-regulates occludin and claudin-1 by activating the MAP kinase (MAPK) pathway, and thus protects the epithelial barrier function of Caco-2 monolayer under hypoxia condition. PMID: 25999789
- EDEM2 and OS-9 are required for ER-associated degradation of non-glycosylated sonic hedgehog PMID: 24910992
- Unique regions mediate the interaction between OS-9 and GRP94. PMID: 24899641
- It delivers mutant neuroserpin to ERAD by recognition of glycan side chains and provide the first in vivo proof of involvement of ERAD in degradation of mutant neuroserpin. PMID: 24795221
- long non-coding RNA ENST00000480739 suppresses tumour cell invasion by regulating OS-9 and HIF-1alpha in pancreatic ductal adenocarcinoma. PMID: 25314054
- Data indicate that the interaction of OS-9 and XTP3-B with CD147(CG) was inhibited by mutations to conserved residues in their lectin domains. PMID: 23097496
- OS-9 plays no direct functional role in HIF degradation since physical interaction of OS-9 with oxygen sensing HIF prolyl hydroxylases cannot occur in vivo due to their different subcellular localization PMID: 21559462
- It regulates endoplasmic reticulum-associated degradation. (review) PMID: 21404621
- The OS-9 specifically recognizes Manalpha1,6Manalpha1,6Man residues on the processed C-arm through the continuous double tryptophan (WW) motif. PMID: 21172656
- The sugar-binding ability of human OS-9 and its involvement in ER-associated degradation PMID: 19914915
- Identification of a peptide, resulting from a point mutation in gene OS-9, recognized by cytolytic T lymphocytes on a human melanoma. PMID: 12747754
- OS-9 regulates the secretory transport of TRPV4 and appears to protect TRPV4 subunits from the precocious ubiquitination and ER-associated degradation. PMID: 17932042
- OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD PMID: 18264092
- Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum inhibit secretion of misfolded protein conformers and enhancing their disposal PMID: 18417469
- OS9 is critically involved in the modulation of ER-to-Golgi transport of DC-STAMP in response to TLR triggering, suggesting a novel role for OS9 in myeloid differentiation and cell fusion. PMID: 18952287
- OS-9 recognises terminally misfolded proteins via polypeptide-based rather than glycan-based signals, but is only required for transferring those bearing N-glycans to the ubiquitination machinery. PMID: 19084021
- model for mannose trimming and the requirement for OS-9 lectin activity in glycoprotein ERAD in which N-glycans lacking the terminal mannose from the C branch are recognized by OS-9 and targeted for degradation PMID: 19346256
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亞細(xì)胞定位：Endoplasmic reticulum lumen.
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蛋白家族：OS-9 family
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組織特異性：Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma SJSA-1 and rhabdomyosarcoma Rh30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
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