MFN1 Recombinant Monoclonal Antibody
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中文名稱：MFN1 Recombinant Monoclonal Antibody
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貨號：CSB-RA062200A0HU
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規格：￥1320
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圖片：
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Flow cytometric analysis of Mitofusin 1 expression in C2C12 cells using Mitofusin 1 antibody. Green, isotype control; red, Mitofusin 1. -
Western blotting analysis using mitofusin 1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mitofusin 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：MFN1; Mitofusin 1; Transmembrane GTPase MFN1; Mitofusin-1; Fzo Homolog; FLJ20693; Mitochondrial Transmembrane GTPase FZO-2; Mitochondrial Transmembrane GTPaseFzo-1; Putative Transmembrane GTPase; EC 3.6.5.-; EC 3.6.5; Hfzo1; Hfzo2
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反應種屬：Human, Mouse, Rat
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免疫原：Recombinant Human MFN1 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：9H12
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 FC 1:200-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
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靶點詳情
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功能：Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity.
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基因功能參考文獻：
- The results lead to a revised understanding of Mfn 1 as single-spanning outer membrane proteins with an Nout-Cin orientation, providing functional insight into the IMS contribution to redox-regulated fusion events. PMID: 29212658
- The results may suggest that TP53BP1 and MFN1 frameshift mutations and their intratumoral heterogeneity (ITH) could contribute to cancer development by inhibiting the TSG activities. PMID: 30082159
- The results show that a metabolic shift from glycolysis in young to mitochondrial respiration in old normal human fibroblasts occurs during chronological lifespan, and MFN1 and OPA1 regulate this process. PMID: 28758339
- mitochondria elongation under hypoxic condition is regulated through SIRT1-mediated MFN1 deacetylation and accumulation. PMID: 28669827
- Regulation of Mfn1 by MGRN1 and the proteasome modulates mitochondrial fusion. PMID: 27713096
- SLC25A46 is a new component in mitochondrial dynamics that serves as a regulator for MFN1/2 oligomerization. PMID: 28057766
- MFN1-positive expression could be seen mainly in ganglion cells after 1 week of minus lens intervention, and with time extension, more and more positive cells appeared in the rod-cone cell and bipolar cell layer, and this phenomenon could not be found in the normal control eyes. PMID: 27609161
- crystal structures of engineered human MFN1 containing the GTPase domain and a helical domain during different stages of GTP hydrolysis; mechanistic model for MFN1-mediated mitochondrial tethering is proposed; results shed light on the molecular basis of mitochondrial fusion and mitofusin-related human neuromuscular disorders PMID: 28114303
- These results suggest that MFN tethers apposing membranes, likely through nucleotide-dependent dimerization. PMID: 27920125
- Improper transcriptional (in)activation of mitofusin-1 and dynamin-related protein 1 during early in vitro embryo development is associated with a decrease in mitochondrial membrane potential and with embryo fragmentation. PMID: 25033890
- miR-19b targets 3'UTR sequences of Mfn1 genes inhibit the expression of Mfn1 PMID: 24824927
- In a amyotrophic lateral sclerosis transgenic mouse model, Mfn1 is significantly increased in spinal cord. PMID: 23713734
- A novel role for the endoplasmic reticulum-associated Gp78 ubiquitin ligase and the Mfn1 mitochondrial fusion factor in mitophagy. PMID: 23427266
- Knock-out of mitofusin protein Mfn1 increased the frequency of mitochondrial fission with increased lifetime of unpaired events whereas deletion of both Mfn1 and Mfn2 resulted in an instable dynamics. PMID: 22649485
- These results collectively suggest a role for Mfn1 in regulating the activation of Bax on the outer mitochondrial membrane in a GTPase-dependent manner. PMID: 22484496
- mitochondrial dynamics, particularly those mediated by the mitofusins, play a role in endothelial cell function and viability. PMID: 21839087
- Patterned Purkinje cell degeneration is dependent on caspase activation, leading to the marked decrease of mitofusion 1 in the transgenic Harlequin cerebellum. PMID: 20974255
- Our data supports a model whereby the translocation of parkin to damaged mitochondria induces the degradation of mitofusin 1 leading to impaired mitochondrial fusion PMID: 21615408
- The impact of mutations in endogenous PINK1 and Parkin on the ubiquitination of mitochondrial fusion and fission factors and the mitochondrial network structure, was investigated. PMID: 21408142
- Gbeta2 also regulated the mobility of Mfn1 on the surface of the mitochondrial membrane and affected the mitochondrial fusion. PMID: 20981029
- Ubiquitination of several mitochondrial proteins, including mitofusin 1 and mitofusin 2 were reduced following the silencing of parkin or PINK1. PMID: 20871098
- Mitofusin degradation by mitochondria-associated Parkin inhibits the fusion of damaged mitochondria with healthy mitochondria to facilitate the selective elimination of the former by autophagy. PMID: 21173115
- MFN1 is required for both the virus-induced redistribution of IPS-1 and IFN production. PMID: 20661427
- the NIC-Akt-Mfn signaling cascade identifies a pathway regulating cell-survival, independent of canonical functions associated with NIC activity PMID: 20339081
- Results show that Fzo homologs mitofusin 1 and 2 are ubiquitous mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. PMID: 11950885
- Mfn1 mediates mitochondrial fusion in human cells PMID: 12475957
- OPA1 functionally requires mitofusin 1 to regulate mitochondrial fusion PMID: 15509649
- Mfn1, Mfn2, NRF-2 and COX IV mRNA were increased 24 h post-exercise in skeletal muscle PMID: 15961417
- Precise interactions between a few proteins are required for mitochondrial fusion and division. Among them Drp1, Mfn1, Mfn2 and Opal are considered the most important. PMID: 17718388
- Fis1 and Mfn1 activities influence mitochondrial signal generation thereby insulin exocytosis. PMID: 18832378
- IL-6 induces Bcl-2 expression to perform cytoprotective functions in response to oxygen toxicity, and that this effect is mediated by alterations in the interactions between Bak and Mfn1/Mfn2. Bcl-2 inhibited the interaction between Bak and Mfn1. PMID: 19168699
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亞細胞定位：Mitochondrion outer membrane; Multi-pass membrane protein.; [Isoform 2]: Cytoplasm.
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蛋白家族：TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, Mitofusin subfamily
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組織特異性：Detected in kidney and heart (at protein level). Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
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數據庫鏈接：
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