CHD1L Recombinant Monoclonal Antibody
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中文名稱：CHD1L Recombinant Monoclonal Antibody
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貨號：CSB-RA293509A0HU
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規格：￥1320
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圖片：
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Flow cytometric analysis of CHD1L expression in HT-1080 cells using CHD1L antibody. Green, isotype control; red, CHD1L. -
Immunocytochemical staining of HT-1080 cells with CHD1L antibody . Nuclei were stained blue with DAPI;CHD1L was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. -
Western blotting analysis using CHD1L antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CHD1L antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. CHD1L, chromodomain helicase DNA binding protein 1 like
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其他：
產品詳情
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Uniprot No.：
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基因名：CHD1L
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別名：CHD1L; Chromodomain Helicase DNA Binding Protein 1 Like; ALC1; Chromodomain-Helicase-DNA-Binding Protein 1-Like; Amplified In Liver Cancer Protein 1; Amplified In Liver Cancer 1; EC 3.6.4.-; EC 3.6.1; CHDL
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反應種屬：Human
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免疫原：Recombinant Human CHD1L protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：21B6
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, FC, ICC
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 FC 1:200-1:2000 ICC 1:100-1:1000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
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基因功能參考文獻：
- CHD1L overexpression was associated with poor prognosis and advanced clinicopathological features, CHD1L may be a valuable biomarker for prognostication of cancer patients. PMID: 30024537
- overexpression of CHD1L in embryonic cells upregulated the expression of ectoderm genes, especially PAX6 PMID: 28946814
- implies a previously unappreciated role for ALC1 in DNA replication, in which ALC1 may regulate replication-fork slowing at CPT-induced DNA-damage sites PMID: 29408941
- Upon DNA damage, binding of PARylated PARP1 by the macro domain induces a conformational change that relieves autoinhibitory interactions with the ATPase motor, which selectively activates ALC1 remodeling upon recruitment to sites of DNA damage. PMID: 29220652
- NAD(+)-metabolite and nucleic acid poly-ADP-ribose triggers ALC1 to drive chromatin relaxation. Modular allostery in this oncogene tightly controls its robust, DNA-damage-dependent activation. PMID: 29220653
- ALC1 is a unique base excision repair factor that functions in a chromatin context, most likely as a chromatin-remodeling enzyme. PMID: 29149203
- CHD1L exerts its anti-apoptotic role through the apoptotic pathway involving caspase-9-caspase-3 apoptotic pathway in MM cells. In addition, we determined that CHD1L expression is increased when MM cells were adhered to fibronectin (FN) or bone marrow stromal cells PMID: 27258734
- CHD1L is involved in the progression of glioma. PMID: 26162969
- Overexpression of CHD1L is positively associated with tumor metastasis of lung adenocarcinoma, and might serve as a novel prognostic biomarker and potential therapeutic target for patients. PMID: 26360781
- This study identified CHD1L as a potential anti-metastasis target for therapeutic intervention in breast cancer. PMID: 26599012
- Relative mRNA expression level of CHD1L was higher in breast cancer cell lines. PMID: 25153161
- These results indicated that CHD1L could serve as a prognostic marker for gastric cancer PMID: 24258459
- CHD1L is now considered to be a novel independent biomarker for progression, prognosis and survival in several solid tumors. [Review] PMID: 24359616
- Data indicate that chromodomain helicase/ATPase DNA-binding protein 1-like (CHD1L) might be an diagnostic and prognostic marker for bladder cancer (BC) patients. PMID: 23807680
- TRIM33 plays a role in PARP-dependent DNA damage response and regulates ALC1 activity by promoting its timely removal from sites of DNA damage. PMID: 23926104
- CHD1L is the target oncogene within the 1q21 amplicon and plays a pivotal role in colorectal carcinoma pathogenesis. PMID: 23746766
- positive expression of CHD1L protein is significantly correlated with the metastasis proceeding of ovarian carcinoma, and CHD1L protein expression, as examined by IHC, may act as a novel prognostic biomarker for patients with ovarian carcinoma. PMID: 23020525
- the model that PAR present on PARylated PARP1 acts as an allosteric effector of ALC1 nucleosome remodeling activity. PMID: 23132853
- data support a model in which poly(ADP-ribosyl)ation of DDB2 suppresses DDB2 ubiquitylation and outline a molecular mechanism for PARP1-mediated regulation of nucleotide excision repair through DDB2 stabilization and recruitment of the chromatin remodeler ALC1 PMID: 23045548
- Mutation in CHD1L is associated with congenital anomalies of the kidneys and urinary tract. PMID: 22146311
- CHD1L/TCTP/Cdc25C/Cdk1 molecular pathway causes the malignant transformation of hepatocytes with the phenotypes of accelerated mitotic progression and the production of aneuploidy PMID: 21953552
- 1q21.1 copy number variant (CNV) results in newly identified function such as decatenation (chromatid untangling) checkpoint (DCC) activation in the case of CHD1l/ALC1 in lymphoblast cell lines. PMID: 21824431
- Overexpression of CHD1L was significantly associated with tumour microsatellite formation, advanced tumour stage, overall survival time of patients who received transarterial chemoembolisation treatment and chemoresistance in hepatocellular carcinoma. PMID: 21068133
- CHD1L promotes hepatocellular carcinoma progression and metastasis in mice and is associated with these processes in human patients. PMID: 20335658
- ALC1 is the target oncogene within the chromosome 1q21 amplicon and plays a pivotal role in hepeatocellular carcinoma pathogenesis. PMID: 18023026
- results define ALC1 as a DNA damage-response protein whose role in this process is sustained by its association with known DNA repair factors and its rapid poly(ADP-ribose)-dependent recruitment to DNA damage sites PMID: 19661379
- Poly(ADP-ribosyl)ation directs recruitment and activation of the ATP-dependent chromatin remodeler ALC1 PMID: 19666485
- Alc1 is a chromatin remodeling enzyme activated by binding of its macrodomain to poly(ADP-ribosyl)ated Parp1. Alc1 is recruited to nucleosomes in vitro and to chromatin in cells when Parp1 catalyzes poly(ADP-ribose) synthesis at sites of DNA damage. PMID: 19666485
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亞細胞定位：Nucleus.
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蛋白家族：SNF2/RAD54 helicase family
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組織特異性：Frequently overexpressed in hepatomacellular carcinomas.
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數據庫鏈接：
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