ABCE1 Recombinant Monoclonal Antibody

說明書

中文名稱：

ABCE1 Recombinant Monoclonal Antibody
貨號：

CSB-RA443691A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of ABCE1 expression in C2C12 cells using ABCE1 antibody. Green, isotype control; red, ABCE1.

Immunocytochemical staining of HepG2 cells with ABCE1 antibody. Nuclei were stained blue with DAPI; ABCE1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using ABCE1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCE1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P61221
基因名：

ABCE1
別名：

ABCE1; ATP Binding Cassette Subfamily E Member 1; RLI; RNASEL1; RNASELI; RNS4I; OABP; RLI1; ATP-Binding Cassette, Sub-Family E (OABP), Member 1; ATP-Binding Cassette Sub-Family E Member 1; 2'-5'-Oligoadenylate-Binding Protein; Ribonuclease 4 Inhibitor; RNase L Inhibitor 1; HuHP68; Ribonuclease L (2',5'-Oligoisoadenylate Synthetase-Dependent) Inhibitor; RNase L Inhibitor; EC 3.6.5.-; ABC38
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human ABCE1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

16E10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway. Together with PELO and HBS1L, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes. Together with PELO and HBS1L, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway. Plays a role in the regulation of mRNA turnover. Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA. As part of the PINK1-regulated signaling, ubiquitinated by CNOT4 upon mitochondria damage; this modification generates polyubiquitin signals that recruit autophagy receptors to the mitochondrial outer membrane and initiate mitophagy. RNASEL-specific protein inhibitor which antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) to RNASEL. Negative regulator of the anti-viral effect of the interferon-regulated 2-5A/RNASEL pathway.; (Microbial infection) May act as a chaperone for post-translational events during HIV-1 capsid assembly.; (Microbial infection) Plays a role in the down-regulation of the 2-5A/RNASEL pathway during encephalomyocarditis virus (EMCV) and HIV-1 infections.
基因功能參考文獻：
1. Induction of ribosome rescue factors PELO and HBS1L is required to support protein synthesis when ABCE1 levels fall in platelets, including for hemoglobin production during blood cell development. PMID: 27681415
2. ABCE1 is involved in histone biosynthesis and DNA replication and therefore is essential for normal S phase progression. PMID: 26985706
3. The present study reported on the effects of ABCE1 overexpression on lung adenocarcinoma cells in vitro, demonstrating its enhancing effect on cell proliferation and invasiveness with simultaneous downregulation of p27 protein expression. PMID: 27314749
4. ABCE1 plays an essential role in the progression and metastasis of lung cancers and may represent a valuable therapeutic target for the management of lung tumor PMID: 26733164
5. miR-299-3p promotes the sensibility of lung cancer to doxorubicin through suppression of ABCE1, at least partly. Therefore, the disordered decreased of miR-299-3p and resulting ABCE1 up-expression may contribute to chemoresistance of lung cancer PMID: 26617714
6. Expression of the ABCE1-silencing gene, transfected by electrotransfer, could inhibit the proliferation, invasion, and migration of thyroid cancer cells. PMID: 26600528
7. Downregulation of ABCE1 via siRNA affects the sensitivity of lung cancer cells against chemotherapeutic agents. PMID: 25744244
8. ABCE1 is closely associated with cell proliferation, invasion and migration in esophageal cancer and silencing the ABCE1 gene by electroporation can significantly reduce the proliferation, invasion and migration capacity PMID: 25815591
9. ABCE1 is able to suppress RNA silencing in Nicotiana benthamiana plants, in mammalian HEK293 cells and in the worm Caenorhabditis elegans. PMID: 25659154
10. ABCE1 is closely connected with the pathogenesis and development of oral cancer, which acts through the cellular pathways of 2-5A/RNase L. PMID: 25337191
11. ABCE1 is closely connected with the pathogenesis and development of esophageal carcinoma, which act through the cellular pathways of 2-5A/RNase L. PMID: 24551278
12. Studies indicate a strong sequence conservation of ABC-type ATPase ABCE1 in eukaryotes and archaea. PMID: 23266104
13. ABCE1 protein regulates a broad range of biological functions including viral infection, tumor cell proliferation, and antiapoptosis. (Review) PMID: 23008114
14. Pulse chase experiments and immunoelectron microscopy shows that HIV-1 Gag associates transiently with ABCE1 during HIV-1 capsid assembly, including at the plasma membrane where assembly is completed. PMID: 17233757
15. HIV-1, HIV-2, SIV mac239, and SIVagm Gag proteins form ABCE1-containing assembly intermediates during immature capsid formation, indicating that Gag proteins of these diverse retroviruses bind to ABCE1 despite the limited homology between these Gags. PMID: 14747530
16. For binding of WT HIV-1 Gag to the cellular ATPase ABCE1, which facilitates HIV-1 capsid assembly, the basic residues in the nucleocapsid domain of Gag are required but the cysteine and histidine residues in the nucleocapsid domain are dispensable. PMID: 16275648
17. During assembly of an immature HIV-1 capsid, HIV-1 Gag progresses through a pathway of sequential assembly intermediates that contain ABCE1, a cellular ATPase that was found to facilitate capsid formation. PMID: 11780123
18. To form HIV-1 capsid assembly intermediates, HIV-1 Gag co-opts a complex found in infected and uninfected cells that contains the cellular ATPase ABCE1 and the RNA helicase DDX6, both of which facilitate HIV-1 capsid assembly. PMID: 22851315
19. Report high expression level of ABCE1 mRNA and protein in human lung adenocarcinoma tissues and metastatic lymph nodes, which was also correlated with clinical stages. PMID: 22267055
20. Pelota/Hbs1 induced dissociation of elongation complexes from ribosomes and release of peptidyl-tRNA, but only in the presence of ABCE1. PMID: 21448132
21. Results suggest that ABCE1 plays an important role in the pathogenesis of human small cell lung cancer cell. PMID: 20372810
22. NTP hydrolysis by ABCE1 is stimulated by posttermination complexes and is required for its ribosomal recycling activity. PMID: 20122402
23. were able to confirm the excess of rare genetic variation among HIV-1-positive African-American individuals (n=53; Tajima's D=-2.34). These results highlight the potential importance of ABCE1's role in infectious diseases such as HIV-1. PMID: 19657357
24. HIV-2 Gag associates with human HP68 in a cell-free system and that Gag proteins of HIV-2, simian immunodeficiency virus SIVmac239, and SIVagm associate with endogenous HP68 in primate cells, as is seen for HIV-1. PMID: 14747530
25. overexpression in a permissive cell line had no significant effect on varicella zoster virus replication PMID: 15107989
26. ABCE1 and its peptides could be target molecules in specific immunotherapy for HLA-A2(+) colon cancer patients. PMID: 15809757
27. The primary role of ABCE-1 in the effect of TULA appears to be the recruitment of TULA to the sites of HIV-1 assembly where TULA interferes with the late steps of the HIV-1 life cycle. PMID: 18006034
28. host protein essential for assembly of immature HIV-1 capsids PMID: 11780123
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亞細胞定位：

Cytoplasm. Mitochondrion. Note=Localized to clusters of virus formation at the plasma membrane.
蛋白家族：

ABC transporter superfamily, ABCE family
數據庫鏈接：

HGNC: 69

OMIM: 601213

KEGG: hsa:6059

STRING: 9606.ENSP00000296577

UniGene: Hs.12013

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